scholarly journals Overexpression of LAMC1 predicts poor prognosis and enhances tumor cell invasion and migration in hepatocellular carcinoma

2017 ◽  
Vol 8 (15) ◽  
pp. 2992-3000 ◽  
Author(s):  
Yaojun Zhang ◽  
Shaoyan Xi ◽  
Jinbin Chen ◽  
Dongsheng Zhou ◽  
Hengjun Gao ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Tumor Cell ◽  
Cell Invasion ◽  
Poor Prognosis ◽  
Tumor Cell Invasion ◽  
Invasion And Migration ◽  
And Migration

scholarly journals MENA promotes esophageal squamous cell carcinoma cell invasion and migration via MMP-2, MMP-9 and AKT activation

2020 ◽  
Author(s):  
Yueheng Li ◽  
Na Gao ◽  
Jing Li ◽  
Zhengfan Gao ◽  
Zhenzhen Yang ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Invasion ◽  
Tumor Cell Invasion ◽  
Wound Healing Assay ◽  
Transwell Assay ◽  
Invasion And Migration ◽  
Akt Activation ◽  
Qrt Pcr ◽  
And Migration ◽  
Migration Capacity

Abstract Background: Esophageal squamous cell carcinoma (ESCC) is a fatal disease with poor prognosis. The predominant reason for ESCC-related death is metastasis caused by tumor cell invasion. Human MENA protein is a member of Ena/Vasp family, which plays a critical role during tumor cell invasion. However, the biological effect of MENA in ESCC cell lines remains unclear Methods: In this study, fluorescent quantitative real-time PCR (qRT-PCR) were conducted to detect the mRNA expression of MENA in tumor and para-cancer tissue, CCK-8 assay and clone formation assay were conducted to evaluate cell proliferation activity, Transwell assay and wound-healing assay were conducted to detect the changes of cell invasion and migration capacity, siRNA and MENA expression vector were constructed to explore biological function of MENA in ESCC cell lines. Western blot analysis were conducted to detect the expressions of MENA , molecular markers of epithelial-mesenchymal transition (EMT), Akt, p-Akt, MMP-2 and MMP-9 respectively in ESCC cell line. Results: The qRT-PCR experiment results showed that MENA expression in ESCC tissue of 35 patients was relatively higher than that in tissue adjacent to cancer. CCK-8 assay suggested that tumor cell proliferation capacity was suppressed followed by the knockdown of MENA expression in Mena high ESCC cell TE13 and was potentiated by the overexpression of MENA in Mena low ESCC cell TE1. Transwell assay and wound healing assay demonstrated that interfering in MENA could inhibit TE13 cells invasion and migration capacity by affecting the expressions of Matrix metalloproteinase-2(MMP-2) and Matrix metalloproteinase-9 (MMP-9), in contrast, overexpression of MENA in Mena low ESCC cell TE1 could promote invasion and migration by up-regulated expression of MMP-2 and MMP-9. Western blot analysis indicated that interfering of MENA expression could affect EMT-related molecular markers (E-cadherin, N-cadherin, Snail, Slug), Akt and p-Akt Conclusions: Our study reveal that MENA could promote the ESCC cell invasion and migration by upregulate MMP-2, MMP-9 expression and Akt activation. Meanwhile, interfering of MENA expression could affect EMT in ESCC cells. This indicated that MENA may be a potential molecular therapeutic target for ESCC metastasis

scholarly journals E2F1 promotes tumor cell invasion and migration through regulating CD147 in prostate cancer

2016 ◽  
Vol 48 (4) ◽  
pp. 1650-1658 ◽  
Author(s):  
YU-XIANG LIANG ◽  
JIAN-MING LU ◽  
RU-JUN MO ◽  
HUI-CHAN HE ◽  
JIAN XIE ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Tumor Cell ◽  
Cell Invasion ◽  
Tumor Cell Invasion ◽  
Invasion And Migration ◽  
And Migration

scholarly journals A Modified and Convenient Method for Assessing Tumor Cell Invasion and Migration and Its Application to Screening for Inhibitors.

1997 ◽  
Vol 20 (4) ◽  
pp. 345-348 ◽  
Author(s):  
Ken-ichi SAITO ◽  
Tohru OKU ◽  
Naomi ATA ◽  
Hirotsugu MIYASHIRO ◽  
Masao HATTORI ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Invasion ◽  
Convenient Method ◽  
Tumor Cell Invasion ◽  
Invasion And Migration ◽  
And Migration

scholarly journals Suppression of PMA-induced tumor cell invasion and migration by ginsenoside Rg1 via the inhibition of NF-κB-dependent MMP-9 expression

2014 ◽  
Vol 32 (5) ◽  
pp. 1779-1786 ◽  
Author(s):  
LI LI ◽  
YIWEN WANG ◽  
BENQUAN QI ◽  
DONGDONG YUAN ◽  
SHUYING DONG ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Invasion ◽  
Tumor Cell Invasion ◽  
Ginsenoside Rg1 ◽  
Invasion And Migration ◽  
And Migration

scholarly journals MicroRNA-484 promotes cell migration and invasion in non-small cell lung cancer

2020 ◽  
Author(s):  
Xiaoning Yang ◽  
Junfeng Ma ◽  
Fanghua Gong ◽  
Yu Liu
Keyword(s):  
Lung Cancer ◽  
Tumor Cell ◽  
Cell Invasion ◽  
Epithelial Mesenchymal Transition ◽  
Small Cell ◽  
Tumor Cell Invasion ◽  
Small Cell Lung ◽  
Mesenchymal Transition ◽  
Invasion And Migration ◽  
And Migration

Abstract Background Lung cancer is one of the most common causes of cancer-related deaths in the world, and non-small cell lung cancer (NSCLC) accounts for 85% of it. Studies have reported that microRNA-484 (miR-484) plays an important regulatory role in carcinogenesis and cancer development. Methods 25 clinical NSCLC samples were collected for microRNA array. The funvtion of miR-484 was investigated through Transwell and Mitgration assays. The expression levers of epithelial-mesenchymal transition (EMT) related factors were assessed by Western blot. Results miR-484 was up-regulated in tissues from NSCLC patients relative to tumor-adjacent normal tissues. Knocking-down miR-484 expression in A549 cells significantly suppressed tumor cell invasion and migration, suppressed epithelial-mesenchymal transition (EMT) process by increasing the expression of E-cadherin, and decreasing the expression of N-cadherin, vimentin, and MMP2. Upregulation of miR-484 expression in BEAS-2B normal lung epithelial cells significantly promoted tumor cell invasion and migration, decreased E-cadherin expression levels, and increased N-cadherin, vimentin, and MMP2 expression levels Conclusion miR-484 can promote tumor cell invasion and migration in NSCLC and may be a new target for NSCLC treatment.

scholarly journals CD155/PVR plays a key role in cell motility during tumor cell invasion and migration

BMC Cancer ◽  
2004 ◽  
Vol 4 (1) ◽  
Author(s):  
Kevin E Sloan ◽  
Brenda K Eustace ◽  
Jean K Stewart ◽  
Carol Zehetmeier ◽  
Claudia Torella ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Motility ◽  
Cell Invasion ◽  
Tumor Cell Invasion ◽  
Invasion And Migration ◽  
And Migration
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