scholarly journals Ets Family Protein, Erg Expression in Developing and Adult Mouse Tissues by a Highly Specific Monoclonal Antibody

2010 ◽  
pp. 197-208 ◽  
Author(s):  
Ahmed A. Mohamed ◽  
Shyh-Han Tan ◽  
Natallia Mikhalkevich ◽  
Sathibalan Ponniah ◽  
Valeri Vasioukhin ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Adult Mouse ◽  
Specific Monoclonal Antibody ◽  
Mouse Tissues ◽  
Family Protein ◽  
Ets Family

scholarly journals Monoclonal antibodies to laminin reveal the heterogeneity of basement membranes in the developing and adult mouse tissues.

1984 ◽  
Vol 98 (3) ◽  
pp. 971-979 ◽  
Author(s):  
Y J Wan ◽  
T C Wu ◽  
A E Chung ◽  
I Damjanov
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Adult Mouse ◽  
Basement Membranes ◽  
Newborn Mouse ◽  
Preimplantation Stage ◽  
Mouse Tissues ◽  
Reichert's Membrane ◽  
Anatomic Locations ◽  
Immunohistochemical Studies

Two monoclonal antibodies raised against laminin isolated from a mouse parietal yolk sac cell line were used for immunohistochemical studies of basement membranes of the mouse embryo and various fetal and adult tissues. No immunoreactivity with either of the two monoclonal antibodies could be detected in the preimplantation-stage embryos, although it has been shown that these embryos contain extracellular laminin reactive with the conventional polyclonal antilaminin antibodies. Reichert's membrane in early postimplantation stages of development reacted with the monoclonal antibody LAM-I but not with the antibody LAM-II. However, from day 8 of pregnancy onward the Reichert's membrane reacted with both antibodies. Basement membranes of the embryo proper were unreactive with both monoclonal antibodies until day 12 of pregnancy. By day 14 some basement membranes of the fetal tissues became reactive with one or both monoclonal antibodies, whereas others remained still unreactive. In the 17-d fetus and the newborn mouse most of the basement membranes reacted with both monoclonal antibodies, whereas others still reacted with only one. Similar heterogeneity in the immunoreactivity of basement membranes of various tissues was noted in the adult mouse as well. These results indicate that the immunoreactivity of laminin in the extracellular matrix changes during development and that the basement membranes in various anatomic locations display heterogeneity even in the adult mouse.

Therapeutic Potential of SARS-CoV-2-Specific Monoclonal Antibody CR3022

2020 ◽  
Author(s):  
Caroline Atyeo ◽  
Matthew D. Slein ◽  
Stephanie Fischinger ◽  
John Burke ◽  
Alexandra Schӓfer ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Therapeutic Potential ◽  
Specific Monoclonal Antibody

SIRPα-specific monoclonal antibody enables antibody-dependent phagocytosis of neuroblastoma cells

2021 ◽  
Author(s):  
Meriem Bahri ◽  
Sareetha Kailayangiri ◽  
Sarah Vermeulen ◽  
Natacha Galopin ◽  
Claudia Rossig ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Neuroblastoma Cells ◽  
Specific Monoclonal Antibody

scholarly journals A new reliable and highly specific monoclonal antibody to detect the C‐terminal region of Silencer of Cytokine Signaling 1 (SOCS1)

2021 ◽  
Author(s):  
Stephanie E. Weissinger ◽  
Malena Zahn ◽  
Ralf Marienfeld ◽  
Claudia Tessmer ◽  
Gerhard Moldenhauer ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Terminal Region ◽  
Cytokine Signaling ◽  
Specific Monoclonal Antibody

scholarly journals Development of a Specific Monoclonal Antibody to Detect Male Cells Expressing the RPS4Y1 Protein

2021 ◽  
Vol 22 (4) ◽  
pp. 2001
Author(s):  
Silvia Spena ◽  
Chiara Cordiglieri ◽  
Isabella Garagiola ◽  
Flora Peyvandi
Keyword(s):  
Monoclonal Antibody ◽  
Prenatal Diagnosis ◽  
Maternal Blood ◽  
Limiting Factors ◽  
Inherited Disease ◽  
Specific Monoclonal Antibody ◽  
Native Form ◽  
Fetal Cells ◽  
Non Invasive ◽  
Reliable Isolation

Hemophilia is an X-linked recessive bleeding disorder. In pregnant women carrier of hemophilia, the fetal sex can be determined by non-invasive analysis of fetal DNA circulating in the maternal blood. However, in case of a male fetus, conventional invasive procedures are required for the diagnosis of hemophilia. Fetal cells, circulating in the maternal bloodstream, are an ideal target for a safe non-invasive prenatal diagnosis. Nevertheless, the small number of cells and the lack of specific fetal markers have been the most limiting factors for their isolation. We aimed to develop monoclonal antibodies (mAbs) against the ribosomal protein RPS4Y1 expressed in male cells. By Western blotting, immunoprecipitation and immunofluorescence analyses performed on cell lysates from male human hepatoma (HepG2) and female human embryonic kidney (HEK293) we developed and characterized a specific monoclonal antibody against the native form of the male RPS4Y1 protein that can distinguish male from female cells. The availability of the RPS4Y1-targeting monoclonal antibody should facilitate the development of novel methods for the reliable isolation of male fetal cells from the maternal blood and their future use for non-invasive prenatal diagnosis of X-linked inherited disease such as hemophilia.

scholarly journals Use of Ly6G-specific monoclonal antibody to deplete neutrophils in mice

2007 ◽  
Vol 83 (1) ◽  
pp. 64-70 ◽  
Author(s):  
Jean M. Daley ◽  
Alan A. Thomay ◽  
Michael D. Connolly ◽  
Jonathan S. Reichner ◽  
Jorge E. Albina
Keyword(s):  
Monoclonal Antibody ◽  
Specific Monoclonal Antibody
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