scholarly journals Lower activation of caspase-1 by Staphylococcus epidermidis isolated from prosthetic joint infections compared to commensals

2018 ◽  
Vol 3 (1) ◽  
pp. 10-14 ◽  
Author(s):  
Emeli Månsson ◽  
Berolla Sahdo ◽  
Åsa Nilsdotter-Augustinsson ◽  
Eva Särndahl ◽  
Bo Söderquist
Keyword(s):  
Staphylococcus Epidermidis ◽  
Normal Skin ◽  
Human Neutrophils ◽  
Caspase 1 ◽  
Joint Infections ◽  
Prosthetic Joint ◽  
Prosthetic Joint Infections ◽  
Skin Flora ◽  
Number Of Individuals ◽  
Active Caspase

Abstract. Nosocomial sequence types of Staphylococcus epidermidis dominate in prosthetic joint infections. We examined caspase-1 activation in human neutrophils after incubation with Staphylococcus epidermidis isolated from prosthetic joint infections and normal skin flora. Active caspase-1 was lower after incubation with isolates from prosthetic joint infections than after incubation with commensal isolates. Both host and isolate dependent differences in active caspase-1 were noted. Our results indicate that there might be a host-dependent incapacity to elicit a strong caspase-1 response towards certain strains of S. epidermidis. Further experiments with a larger number of individuals are warranted.

Staphylococcus epidermidis from prosthetic joint infections induces lower IL-1β release from human neutrophils than isolates from normal flora

Apmis ◽  
2018 ◽  
Vol 126 (8) ◽  
pp. 678-684
Author(s):  
Emeli Månsson ◽  
Bo Söderquist ◽  
Åsa Nilsdotter-Augustinsson ◽  
Eva Särndahl ◽  
Isak Demirel
Keyword(s):  
Staphylococcus Epidermidis ◽  
Human Neutrophils ◽  
Normal Flora ◽  
Joint Infections ◽  
Prosthetic Joint ◽  
Prosthetic Joint Infections

scholarly journals Genomic Analysis of Cutibacterium acnes Strains Isolated from Prosthetic Joint Infections

2021 ◽  
Vol 9 (7) ◽  
pp. 1500
Author(s):  
Llanos Salar-Vidal ◽  
Yvonne Achermann ◽  
John-Jairo Aguilera-Correa ◽  
Anja Poehlein ◽  
Jaime Esteban ◽  
...  
Keyword(s):  
Normal Skin ◽  
Genomic Analysis ◽  
Healthy Skin ◽  
Common Cause ◽  
Good Outcome ◽  
Joint Infections ◽  
Prosthetic Joint ◽  
Prosthetic Joint Infections ◽  
Cutibacterium Acnes ◽  
Clinical Conditions

Cutibacterium acnes is a common cause of prosthetic joint infections (PJIs). The C. acnes population can be divided into six main phylotypes (IA1, IA2, IB, IC, II and III) that are associated with different clinical conditions and normal skin. A single-locus sequence typing (SLST) scheme can distinguish ten main SLST types: A-E (all IA1), F (IA2), G (IC), H (IB), K (II), L (III). We genome-sequenced and compared 16 strains of C. acnes isolated from healthy skin (n = 4) and PJIs (n = 12), including six PJI cases with a good outcome (four shoulder PJIs, one hip PJI, one knee PJI) and six with infection relapse (three shoulder PJIs, three hip PJIs). The sequenced strains belonged to four different phylotypes (IA1, IA2, IB and II) and seven different SLST types. All five type IB strains (all SLST type H1) were PJI isolates (three hip PJIs, two shoulder PJIs), and four of these caused infection relapse (three hip PJIs, one shoulder PJI). Isolates from PJI cases with a good outcome belonged to three different phylotypes (IA, IB, II). Interestingly, four strains (three strains from PJI cases with good outcome and one strain from healthy skin) contained a linear plasmid; these strains belonged to different SLST types (A1, C1, F4, H1) and were isolated in three different hospitals. This study suggests that type IB strains have the potential to cause infection relapse, in particular regarding hip PJIs. Moreover, our study revealed that strains belonging to the same SLST type can differ in their accessory genome in different geographic locations, indicative of microevolution.

scholarly journals Comparative genomics of Staphylococcus epidermidis from prosthetic-joint infections and nares highlights genetic traits associated with antimicrobial resistance, not virulence

2021 ◽  
Author(s):  
Emeli Månsson ◽  
Thor Bech Johannesen ◽  
Åsa Nilsdotter-Augustinsson ◽  
Bo Söderquist ◽  
Marc Stegger
Keyword(s):  
Population Structure ◽  
Antimicrobial Resistance ◽  
Staphylococcus Epidermidis ◽  
Type Species ◽  
Genetic Traits ◽  
Content Type ◽  
Link Type ◽  
Joint Infections ◽  
Prosthetic Joint ◽  
Prosthetic Joint Infections

There is increased awareness of the worldwide spread of specific epidemic multidrug-resistant (MDR) lineages of the human commensal Staphylococcus epidermidis . Here, using bioinformatic analyses accounting for population structure, we determined genomic traits (genes, SNPs and k-mers) that distinguish S. epidermidis causing prosthetic-joint infections (PJIs) from commensal isolates from nares, by analysing whole-genome sequencing data from S. epidermidis from PJIs prospectively collected over 10 years in Sweden, and contemporary S. epidermidis from the nares of patients scheduled for arthroplasty surgery. Previously suggested virulence determinants and the presence of genes and mutations linked to antimicrobial resistance (AMR) were also investigated. Publicly available S. epidermidis sequences were used for international extrapolation and validation of findings. Our data show that S. epidermidis causing PJIs differed from nasal isolates not by virulence but by traits associated with resistance to compounds used in prevention of PJIs: β-lactams, aminoglycosides and chlorhexidine. Almost a quarter of the PJI isolates did not belong to any of the previously described major nosocomial lineages, but the AMR-related traits were also over-represented in these isolates, as well as in international S. epidermidis isolates originating from PJIs. Genes previously associated with virulence in S. epidermidis were over-represented in individual lineages, but failed to reach statistical significance when adjusted for population structure. Our findings suggest that the current strategies for prevention of PJIs select for nosocomial MDR S. epidermidis lineages that have arisen from horizontal gene transfer of AMR-related traits into multiple genetic backgrounds.

scholarly journals Partial atlE Sequencing of Staphylococcus epidermidis Strains from Prosthetic Joint Infections

2009 ◽  
Vol 47 (7) ◽  
pp. 2321-2324 ◽  
Author(s):  
V. Sivadon ◽  
M. Rottman ◽  
J.-C. Quincampoix ◽  
E. Prunier ◽  
M. Le Moal ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Joint Infections ◽  
Prosthetic Joint ◽  
Prosthetic Joint Infections

scholarly journals Bacterial Extracellular DNA Production Is Associated with Outcome of Prosthetic Joint Infections

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Beata Zatorska ◽  
Carla Renata Arciola ◽  
Nicolas Haffner ◽  
Luigi Segagni Lusignani ◽  
Elisabeth Presterl ◽  
...  
Keyword(s):  
Clinical Outcome ◽  
Staphylococcus Epidermidis ◽  
Joint Infection ◽  
Surrogate Marker ◽  
Predictive Marker ◽  
Extracellular Dna ◽  
Joint Infections ◽  
Prosthetic Joint ◽  
Prosthetic Joint Infections ◽  
Dna Production

In a retrospective study the association of the production of extracellular DNA (eDNA) in biofilms of clinical staphylococcal isolates from 60 patients with prosthetic joint infection (PJI) and the clinical outcome were investigated. Data from a previous study on eDNA production determined in 24-hour biofilms of staphylococcal isolates (Staphylococcus aureusn=30,Staphylococcus epidermidisn=30) was correlated with the patients’ clinical outcome after 3 and 12 months. Statistical analysis was performed using either the Spearman’s rank correlations test or the t-test. eDNA production ofS. epidermidisin 24-hour biofilms correlated with the patients’ outcome ‘not cured‘ after 12 months. ForS. aureusno such correlation was detected. Thus, eDNA may be a virulence factor ofS. epidermidis.Quantification of eDNA production as a surrogate marker for biofilm formation might be a potential predictive marker for the management of PJI.

scholarly journals Genotypic and phenotypic changes of Staphylococcus epidermidis during relapse episodes in prosthetic joint infections

2019 ◽  
Vol 51 (2) ◽  
pp. 601-612
Author(s):  
Silvestre Ortega-Peña ◽  
Rafael Franco-Cendejas ◽  
Alejandra Aquino-Andrade ◽  
Gabriel Betanzos-Cabrera ◽  
Ashutosh Sharma ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Phenotypic Changes ◽  
Joint Infections ◽  
Prosthetic Joint ◽  
Prosthetic Joint Infections

scholarly journals Staphylococcus saccharolyticus Associated with Prosthetic Joint Infections: Clinical Features and Genomic Characteristics

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 397
Author(s):  
Bo Söderquist ◽  
Mastaneh Afshar ◽  
Anja Poehlein ◽  
Holger Brüggemann
Keyword(s):  
Aseptic Loosening ◽  
Normal Skin ◽  
Low Grade ◽  
Pathogenic Potential ◽  
Long Term Outcome ◽  
Joint Infections ◽  
Prosthetic Joint ◽  
Prosthetic Joint Infections ◽  
Cutibacterium Acnes

The anaerobic coagulase-negative staphylococcal species Staphylococcus saccharolyticus is a member of the normal skin microbiota. However, S. saccharolyticus is rarely found in clinical specimens and its pathogenic potential is unclear. The clinical data of prosthetic hip (n = 5) and shoulder (n = 2) joint implant-associated infections where S. saccharolyticus was detected in periprosthetic tissue specimens are described. The prosthetic hip joint infection cases presented as “aseptic” loosening and may represent chronic, insidious, low-grade prosthetic joint infections (PJIs), eventually resulting in loosening of prosthetic components. All cases were subjected to one-stage revision surgery and the long-term outcome was good. The shoulder joint infections had an acute onset. Polymicrobial growth, in all cases with Cutibacterium acnes, was found in 4/7 patients. All but one case were treated with long-term administration of beta-lactam antibiotics. Whole-genome sequencing (WGS) of the isolates was performed and potential virulence traits were identified. WGS could distinguish two phylogenetic clades (clades 1 and 2), which likely represent distinct subspecies of S. saccharolyticus. Little strain individuality was observed among strains from the same clade. Strains of clade 2 were exclusively associated with hip PJIs, whereas clade 1 strains originated from shoulder PJIs. It is possible that strains of the two clades colonize different skin habitats. In conclusion, S. saccharolyticus has the potential to cause PJIs that were previously regarded as aseptic loosening of prosthetic joint devices.

scholarly journals Staphylococcus epidermidis isolates from nares and prosthetic joint infections are mupirocin susceptible

2018 ◽  
Vol 3 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Lavin Salih ◽  
Staffan Tevell ◽  
Emeli Månsson ◽  
Åsa Nilsdotter-Augustinsson ◽  
Bengt Hellmark ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Antimicrobial Agents ◽  
Multi Drug Resistance ◽  
Disc Diffusion ◽  
Diffusion Test ◽  
Joint Replacements ◽  
Joint Infections ◽  
Prosthetic Joint ◽  
Prosthetic Joint Infections

Abstract. The objective of the present study was to investigate the antibiotic susceptibility including mupirocin among Staphylococcus. epidermidis isolated from prosthetic joint infections (PJIs) (n=183) and nasal isolates (n=75) from patients intended to undergo prosthetic joint replacements. Susceptibility to mupirocin (used for eradication of nasal carriership of Staphylococcus aureus) was investigated by gradient test, and susceptibility to various other antimicrobial agents was investigated by disc diffusion test. All isolates, except three from PJIs and one from the nares, were fully susceptible to mupirocin. Multi-drug resistance (≥3 antibiotic classes) was found in 154/183 (84.2%) of the PJI isolates but only in 2/75 (2.7%) of the nares isolates, indicating that S. epidermidis causing PJIs do not originate from the nares.

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