scholarly journals AKR1C3 Overexpression Mediates Methotrexate Resistance in Choriocarcinoma Cells

2014 ◽  
Vol 11 (11) ◽  
pp. 1089-1097 ◽  
Author(s):  
Jing Zhao ◽  
Yang Xiang ◽  
Changji Xiao ◽  
Peng Guo ◽  
Dan Wang ◽  
...  
Keyword(s):  
Methotrexate Resistance ◽  
Choriocarcinoma Cells

scholarly journals Decreased folylpolyglutamate synthetase activity as a mechanism of methotrexate resistance in CCRF-CEM human leukemia sublines.

1991 ◽  
Vol 266 (10) ◽  
pp. 6181-6187 ◽  
Author(s):  
D E McCloskey ◽  
J J McGuire ◽  
C A Russell ◽  
B G Rowan ◽  
J R Bertino ◽  
...  
Keyword(s):  
Synthetase Activity ◽  
Human Leukemia ◽  
Folylpolyglutamate Synthetase ◽  
Methotrexate Resistance

scholarly journals Choriocarcinoma cells increase the number of differentiating human cytotrophoblasts through an in vitro interaction

1991 ◽  
Vol 266 (13) ◽  
pp. 8517-8522
Author(s):  
A. Hochberg ◽  
C. Sibley ◽  
M. Pixley ◽  
Y. Sadovsky ◽  
B. Strauss ◽  
...  
Keyword(s):  
Choriocarcinoma Cells ◽  
In Vitro Interaction

scholarly journals The biosynthesis, processing, and secretion of laminin by human choriocarcinoma cells.

1985 ◽  
Vol 260 (27) ◽  
pp. 14732-14742 ◽  
Author(s):  
B P Peters ◽  
R J Hartle ◽  
R F Krzesicki ◽  
T G Kroll ◽  
F Perini ◽  
...  
Keyword(s):  
Choriocarcinoma Cells ◽  
Human Choriocarcinoma Cells

scholarly journals Silencing of the Gene for the α-Subunit of Human Chorionic Gonadotropin by the Embryonic Transcription Factor Oct-3/4

1997 ◽  
Vol 11 (11) ◽  
pp. 1651-1658 ◽  
Author(s):  
Limin Liu ◽  
Douglas Leaman ◽  
Michel Villalta ◽  
R. Michael Roberts
Keyword(s):  
Transcription Factor ◽  
Binding Site ◽  
Gene Promoter ◽  
Site Directed Mutagenesis ◽  
Mobility Shift ◽  
Α Subunit ◽  
Choriocarcinoma Cells ◽  
Human Choriocarcinoma Cells ◽  
Electrophoretic Mobility Shift Assays ◽  
Jar Cells

Abstract CG is required for maintenance of the corpus luteum during pregnancy in higher primates. As CG is a heterodimeric molecule, some form of coordinated control must be maintained over the transcription of its two subunit genes. We recently found that expression of human CG β-subunit (hCGβ) in JAr human choriocarcinoma cells was almost completely silenced by the embryonic transcription factor Oct-3/4, which bound to a unique ACAATAATCA octameric sequence in the hCGβ gene promoter. Here we report that Oct-3/4 is also a potent inhibitor of hCG α-subunit (hCGα) expression in JAr cells. Oct-3/4 reduced human GH reporter expression from the −170 hCGα promoter in either the presence or absence of cAMP by about 70% in transient cotransfection assays, but had no effect on expression from either the −148 hCGα or the −99 hCGα promoter. Unexpectedly, no Oct-3/4-binding site was identified within the −170 to −148 region of the hCGα promoter, although one was found around position −115 by both methylation interference footprinting and electrophoretic mobility shift assays. Site-directed mutagenesis of this binding site destroyed the affinity of the promoter for Oct-3/4, but did not affect repression of the promoter. Therefore, inhibition of hCGα gene transcription by Oct-3/4 appears not to involve direct binding of this factor to the site responsible for silencing. When stably transfected into JAr cells, Oct-3/4 reduced the amounts of both endogenous hCGα mRNA and protein by 70–80%. Oct-3/4 is therefore capable of silencing both hCGα and hCGβ gene expression. We suggest that as the trophoblast begins to form, reduction of Oct-3/4 expression permits the coordinated onset of transcription from the hCGα and hCGβ genes.

scholarly journals Expression of the Germ Cell Alkaline Phosphatase Gene in Human Choriocarcinoma Cells

1989 ◽  
Vol 264 (21) ◽  
pp. 12611-12619
Author(s):  
S Watanabe ◽  
T Watanabe ◽  
W B Li ◽  
B W Soong ◽  
J Y Chou
Keyword(s):  
Alkaline Phosphatase ◽  
Germ Cell ◽  
Choriocarcinoma Cells ◽  
Human Choriocarcinoma Cells

Uptake and Degradation of Plasma Lipoproteins by Human Choriocarcinoma Cells in Culture*

Endocrinology ◽  
1979 ◽  
Vol 104 (1) ◽  
pp. 8-16 ◽  
Author(s):  
E. R. SIMPSON ◽  
D. W. BILHEIMER ◽  
P. C. MACDONALD ◽  
J. C. PORTER
Keyword(s):  
Plasma Lipoproteins ◽  
Cells In Culture ◽  
Choriocarcinoma Cells ◽  
Human Choriocarcinoma Cells
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