scholarly journals miR-143 is implicated in growth plate injury by targeting IHH in precartilaginous stem cells

2021 ◽  
Vol 18 (9) ◽  
pp. 1999-2007
Author(s):  
Fu-Yong Zhang ◽  
Yun-Fang Zhen ◽  
Zhi-Xiong Guo ◽  
Jin Dai ◽  
Lun-Qing Zhu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Plate ◽  
Precartilaginous Stem Cells ◽  
Growth Plate Injury

scholarly journals The effect of stem cells in repairing growth plate injury: a systematic review and meta-analysis

2021 ◽  
Author(s):  
Xiao-long Xu ◽  
Sheng-jun Lu ◽  
Hong Pei ◽  
Shun-guang Chen ◽  
Quan-ming Liao
Keyword(s):  
Systematic Review ◽  
Stem Cells ◽  
Stem Cell ◽  
Growth Plate ◽  
Meta Analysis ◽  
Cochrane Library ◽  
Cell Group ◽  
Control Group ◽  
Angular Deformity ◽  
Growth Plate Injury

Abstract Background: Multiple studies have focused on stem cell-based therapies for growth plate injury.However, the results are not consistent.Objectives: This systematic review and meta-analysis were performed to evaluate the effects of stem cells on growth plate healing.Methods: A detailed search of relevant studies was conducted in three databases including Pub med, Cochrane library, and Embase databases, using the following keywords: “growth plate” or “physis” AND “stem cell” from inception to November 10, 2021. The standard mean difference (SMD) and 95% confidence interval (CI) for each individual study were extracted from the original studies based on relevant data and pooled to obtain integrated estimates using random effects modeling.Results: A total of 6 studies were identified. The results demonstrated that the angular deformity in the stem cell group was significantly lower than that in the control group at 4, 8,12 and 16weeks. The length discrepancy represented the degree of shortening deformity. In the stem cell group, the shortening deformity was milder than that of the control group at 16weeks. Meanwhile, at 16 weeks after surgery, the higher histologic scores in the stem cell group indicated that stem cell can significantly improve the repair quality of growth plate.Conclusions: This systematic review and meta-analysis confirmed that stem cell improved the rehabilitation of growth plate injury. However, larger-scale studies are needed to further support these findings.

Isolation, Culture and Identification of Bovine Skeletal Stem Cells

2021 ◽  
Vol 11 (12) ◽  
pp. 2337-2345
Author(s):  
Junhui Lai ◽  
Qin Yang ◽  
Ruining Liang ◽  
Weijun Guan ◽  
Xiuxia Li
Keyword(s):  
Stem Cells ◽  
Cell Surface ◽  
Bone Formation ◽  
Growth Plate ◽  
Bone Development ◽  
Long Bone ◽  
Biological Characterization ◽  
Self Renewal ◽  
And Cartilage

The growth plate is essential in long bone formation and contains a wealth of skeletal stem cells (SSCs). Though the origin and the mechanism for SSCs generation remain uncertain, recent studies demonstrate the transition from cartilage to bone that in the lineage for bone development. SSCs possesses the ability to differentiate into bone and cartilage in vitro. In this research, we aimed to isolate and culture the skeletal stem cells from bovine cattle and then studied its biological characterization. The results showed that these bovine SSCs are positive for PDPN+CD73+CD164+CD90+CD44+ cell surface bio-markers, they are capable of self-renewal and differentiation. Our dates proved that SSCs exists in bovine’s long bone.

scholarly journals Local Changes to the Distal Femoral Growth Plate Following Injury in Mice

2017 ◽  
Vol 139 (7) ◽  
Author(s):  
Lauren M. Mangano Drenkard ◽  
Meghan E. Kupratis ◽  
Katie Li ◽  
Louis C. Gerstenfeld ◽  
Elise F. Morgan
Keyword(s):  
Growth Plate ◽  
Skeletal Development ◽  
Global Changes ◽  
Mineral Density ◽  
Injury Site ◽  
Hypertrophic Zone ◽  
Proliferative Zone ◽  
Spatial Changes ◽  
Zonal Organization ◽  
Growth Plate Injury

Injury to the growth plate is associated with growth disturbances, most notably premature cessation of growth. The goal of this study was to identify spatial changes in the structure and composition of the growth plate in response to injury to provide a foundation for developing therapies that minimize the consequences for skeletal development. We used contrast-enhanced microcomputed tomography (CECT) and histological analyses of a murine model of growth plate injury to quantify changes in the cartilaginous and osseous tissue of the growth plate. To distinguish between local and global changes, the growth plate was divided into regions of interest near to and far from the injury site. We noted increased thickness and CECT attenuation (a measure correlated with glycosaminoglycan (GAG) content) near the injury, and increased tissue mineral density (TMD) of bone bridges within the injury site, compared to outside the injury site and contralateral growth plates. Furthermore, we noted disruption of the normal zonal organization of the physis. The height of the hypertrophic zone was increased at the injury site, and the relative height of the proliferative zone was decreased across the entire injured growth plate. These results indicate that growth plate injury leads to localized disruption of cellular activity and of endochondral ossification. These local changes in tissue structure and composition may contribute to the observed retardation in femur growth. In particular, the changes in proliferative and hypertrophic zone heights seen following injury may impact growth and could be targeted when developing therapies for growth plate injury.

Effect of Astragaloside IV On Precartilaginous Stem Cells to Promote Bone Development in Rats

2021 ◽  
Author(s):  
Zhizhou Jiang ◽  
Hang Yin ◽  
Lei Zhao ◽  
Jianyong Jiang ◽  
Jinbo Ni ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cultured Cells ◽  
Bone Development ◽  
Biological Characteristics ◽  
Control Group ◽  
Astragaloside Iv ◽  
Tibial Length ◽  
Antibody Staining ◽  
Precartilaginous Stem Cells ◽  
Experimental Group

Abstract Objective To explore the effect of astragaloside IV in promoting bone development by promoting the proliferation of precartilaginous stem cells. Methods To co-cultured the cells from the resting chondrocyte of growth plate and LaCroix of 24-hours old rats,and identified by FGFR-3 staining. Choosing astragaloside IV induce precartilaginous stem cells cultured in vitro, using Collagen type Ⅱ monoclonal antibody staining and MTT to test cell biological characteristics. Four 4 weeks old SD rats were selected and divided into an experimental group and control group, 24 rats in each group. The rats in the experimental group were injected with astragalus injection in a dose of 8.0g / kg once a day. The rats in the other group were injected with the same amount of normal saline. The 3rd and 5th week after feeding, 12 rats were killed, and the tibial length was measured by vernier caliper.Rusults The FGFR-3 staining was positive, which proved that the cultured cells were precartilaginous stem cells. Collagen typeⅡmonoclonal antibody staining is positive and the OD value detected by MTT test was higher, after astragaloside IV induced the precartilaginous stem cells. After astragaloside IV injection, the tibial length of experimental group measured by vernier caliper was significantly higher than that of the control group.Conclusion astragaloside IV can promote the proliferation and biological characteristics of precartilaginous stem cells, and then promote bone development.

scholarly journals Adipose-derived stromal vascular fraction prevent bone bridge formation on growth plate injury in rat (in vivo studies) an experimental research

2020 ◽  
Vol 60 ◽  
pp. 211-217
Author(s):  
Panji Sananta ◽  
Rahaditya I Gede Made Oka ◽  
Prof Respati Suryanto Dradjat ◽  
Heri Suroto ◽  
Edi Mustamsir ◽  
...  
Keyword(s):  
Growth Plate ◽  
Experimental Research ◽  
Stromal Vascular Fraction ◽  
In Vivo Studies ◽  
Bone Bridge ◽  
Bridge Formation ◽  
Growth Plate Injury

scholarly journals FREQUENCY OF WRIST GROWTH PLATE INJURY IN YOUNG GYMNASTS AT A TRAINING CENTER

2016 ◽  
Vol 24 (4) ◽  
pp. 204-207 ◽  
Author(s):  
María Roxana Viamont Guerra ◽  
Jose Renato Depari Estelles ◽  
Yussef Ali Abdouni ◽  
Diego Figueira Falcochio ◽  
Joao Roberto Polydoro Rosa ◽  
...  
Keyword(s):  
Growth Plate ◽  
Training Center ◽  
Growth Plate Injury

Growth plate injury of the hand and wrist in renal osteodystrophy

1990 ◽  
Vol 19 (7) ◽  
Author(s):  
Mark Arvin ◽  
SusanJ. White ◽  
EthanM. Braunstein
Keyword(s):  
Growth Plate ◽  
Renal Osteodystrophy ◽  
Growth Plate Injury

scholarly journals CD146+ skeletal stem cells from growth plate exhibit specific chondrogenic differentiation capacity in vitro

2017 ◽  
Vol 16 (6) ◽  
pp. 8019-8028 ◽  
Author(s):  
Ying-Xing Wu ◽  
Xing-Zhi Jing ◽  
Yue Sun ◽  
Ya-Ping Ye ◽  
Jia-Chao Guo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Plate ◽  
Chondrogenic Differentiation ◽  
Differentiation Capacity

2034 Genistein in amelioration of radiation-induced epiphyseal growth plate injury in growing rats

2009 ◽  
Vol 7 (2) ◽  
pp. 161
Author(s):  
E. Topkan ◽  
A.A. Yavuz ◽  
R. Erdem ◽  
D. Bacanli ◽  
C. Onal ◽  
...  
Keyword(s):  
Growth Plate ◽  
Epiphyseal Growth Plate ◽  
Growing Rats ◽  
Radiation Induced ◽  
Growth Plate Injury
Sign in / Sign up

Export Citation Format

Share Document