scholarly journals Supercritical Carbon Dioxide-decellularized Porcine Acellular Dermal Matrix combined with Autologous Adipose-derived Stem Cells: Its Role in Accelerated Diabetic Wound Healing

2020 ◽  
Vol 17 (3) ◽  
pp. 354-367 ◽  
Author(s):  
Ping-Ruey Chou ◽  
Yun-Nan Lin ◽  
Sheng-Hua Wu ◽  
Sin-Daw Lin ◽  
Periasamy Srinivasan ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Stem Cells ◽  
Wound Healing ◽  
Acellular Dermal Matrix ◽  
Adipose Derived Stem Cells ◽  
Diabetic Wound ◽  
Dermal Matrix ◽  
Porcine Acellular Dermal Matrix ◽  
Diabetic Wound Healing ◽  
Acellular Dermal

Adipose-Derived Stem Cells Seeded on Acellular Dermal Matrix Grafts Enhance Wound Healing in a Murine Model of a Full-Thickness Defect

2012 ◽  
Vol 69 (6) ◽  
pp. 656-662 ◽  
Author(s):  
Sheng-Ping Huang ◽  
Chia-Chun Hsu ◽  
Shun-Cheng Chang ◽  
Chih-Hsin Wang ◽  
Shou-Cheng Deng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Murine Model ◽  
Acellular Dermal Matrix ◽  
Adipose Derived Stem Cells ◽  
Full Thickness ◽  
Dermal Matrix ◽  
Acellular Dermal ◽  
Enhance Wound Healing

Extracellular vesicles derived from adipose-derived stem cells accelerate diabetic wound healing by suppressing the expression of matrix metalloproteinase-9

2021 ◽  
Vol 22 ◽  
Author(s):  
Jiang-wen Wang ◽  
Yuan-zheng Zhu ◽  
Xuan Hu ◽  
Jia-ying Nie ◽  
Zhao-hui Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mouse Model ◽  
Adipose Derived Stem Cells ◽  
Collagen Deposition ◽  
Diabetic Wound ◽  
Diabetic Wound Healing ◽  
Diabetic Wounds ◽  
Metalloproteinase 9

Background: The healing of diabetic wounds is poor due to a collagen deposition disorder. Matrix metalloproteinase-9 (MMP-9) is closely related to collagen deposition in the process of tissue repair. Many studies have demonstrated that extracellular vesicles derived from adipose-derived stem cells (ADSC-EVs) promote diabetic wound healing by enhancing collagen deposition. Objective: In this study, we explored if ADSC-EVs could downregulate the expression of MMP-9 in diabetic wounds and promote wound healing by improving collagen deposition. The potential effects of ADSC-EVs on MMP-9 and diabetic wound healing were tested both in vitro and in vivo. Methods: We first evaluated the effect of ADSC-EVs on the proliferation and MMP-9 secretion of HaCaT cells treated with advanced glycation end product-bovine serum albumin (AGE-BSA), using CCK-8 western blot and MMP-9 enzyme-linked immunosorbent assay(ELISA). Next, the effect of ADSC-EVs on the healing, re-epithelialisation, collagen deposition, and MMP-9 concentration in diabetic wound fluids was evaluated in an immunodeficient mouse model via MMP-9 ELISA and haematoxylin and eosin, Masson’s trichrome, and immunofluorescence staining for MMP-9. Results: In vitro, ADSC-EVs promoted the proliferation and MMP-9 secretion of HaCaT cells.In vivo, ADSC-EVs accelerated diabetic wound healing by improving re-epithelialisation and collagen deposition and by inhibiting the expression of MMP-9. Conclusion: ADSC-EVs possessed the healing of diabetic wounds in a mouse model by inhibiting downregulating MMP-9 and improving collagen deposition.Thus ,ADSC-EVs are a promising candidate for the treatment of diabetic wounds .

Adipose-derived stem cells: Effectiveness and advances in delivery in diabetic wound healing

2018 ◽  
Vol 107 ◽  
pp. 625-633 ◽  
Author(s):  
Mohamed Gadelkarim ◽  
Abdelrahman Ibrahim Abushouk ◽  
Esraa Ghanem ◽  
Ali Mohamed Hamaad ◽  
Anas M. Saad ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Adipose Derived Stem Cells ◽  
Diabetic Wound ◽  
Diabetic Wound Healing

Accelerated diabetic wound healing in a murine model with the application of multipotent human adipose derived stem cells

2006 ◽  
Vol 203 (3) ◽  
pp. S43 ◽  
Author(s):  
Anna M. Parker ◽  
George Rodeheaver ◽  
Lisa Salopek ◽  
Hulan Shang ◽  
Moshe Khurgel ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Murine Model ◽  
Adipose Derived Stem Cells ◽  
Diabetic Wound ◽  
Diabetic Wound Healing
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