scholarly journals Role of Sirolimus in renal tubular apoptosis in response to unilateral ureteral obstruction

2018 ◽  
Vol 15 (13) ◽  
pp. 1433-1442 ◽  
Author(s):  
Mei Yang ◽  
Yang-yang Zhuang ◽  
Wei-wei Wang ◽  
Hai-ping Zhu ◽  
Yan-jie Zhang ◽  
...  
Keyword(s):  
Ureteral Obstruction ◽  
Unilateral Ureteral Obstruction ◽  
Renal Tubular

scholarly journals Role of nitric oxide in renal tubular apoptosis of unilateral ureteral obstruction

2001 ◽  
Vol 59 (4) ◽  
pp. 1290-1303 ◽  
Author(s):  
Akira Miyajima ◽  
Jie Chen ◽  
Dix P. Poppas ◽  
E. Darracott Vaughan ◽  
Diane Felsen
Keyword(s):  
Nitric Oxide ◽  
Ureteral Obstruction ◽  
Unilateral Ureteral Obstruction ◽  
Renal Tubular

scholarly journals The Role of Apelin on the Alleviative Effect of Angiotensin Receptor Blocker in Unilateral Ureteral Obstruction-Induced Renal Fibrosis

Nephron Extra ◽  
2012 ◽  
Vol 2 (1) ◽  
pp. 39-47 ◽  
Author(s):  
Masashi Nishida ◽  
Yasuko Okumura ◽  
Tatsujiro Oka ◽  
Kentaro Toiyama ◽  
Seiichiro Ozawa ◽  
...  
Keyword(s):  
Ureteral Obstruction ◽  
Renal Fibrosis ◽  
Angiotensin Receptor Blocker ◽  
Unilateral Ureteral Obstruction ◽  
Receptor Blocker ◽  
Angiotensin Receptor

scholarly journals Dapagliflozin Alleviates Renal Fibrosis by Inhibiting RIP1-RIP3-MLKL-Mediated Necroinflammation in Unilateral Ureteral Obstruction

2022 ◽  
Vol 12 ◽  
Author(s):  
Mei Ying Xuan ◽  
Shang Guo Piao ◽  
Jun Ding ◽  
Qi Yan Nan ◽  
Mei Hua Piao ◽  
...  
Keyword(s):  
Cell Death ◽  
Ureteral Obstruction ◽  
Renal Fibrosis ◽  
Apoptotic Cell ◽  
Subsequent Development ◽  
Unilateral Ureteral Obstruction ◽  
Apoptotic Cell Death ◽  
Inflammasome Activation ◽  
Renal Tubular

Dapagliflozin, a sodium-glucose cotransporter-2 inhibitor, offers renoprotection in diabetes. However, potential for use in nondiabetic kidney disease remains unknown. Herein, we assessed whether dapagliflozin alleviates renal fibrosis by interfering with necroinflammation in a rat model of unilateral ureteral obstruction (UUO) and in vitro. After induction of UUO, rats were administered dapagliflozin daily for seven consecutive days. UUO induced significant renal tubular necrosis and overexpression of RIP1-RIP3-MLKL axis proteins; these coincided with NLRP3 inflammasome activation, and subsequent development of renal fibrosis. Oxidative stress caused by UUO is tightly associated with endoplasmic reticulum stress and mitochondrial dysfunction, leading to apoptotic cell death through Wnt3α/β-catenin/GSK-3β signaling; all of which were abolished by both dapagliflozin and specific RIP inhibitors (necrostatin-1 and GSK872). In H2O2-treated HK-2 cells, dapagliflozin and RIP inhibitors suppressed overexpression of RIP1-RIP3-MLKL proteins and pyroptosis-related cytokines, decreased intracellular reactive oxygen species production and apoptotic cell death, whereas cell viability was improved. Moreover, activated Wnt3α/β-catenin/GSK-3β signaling was inhibited by dapagliflozin and Wnt/β-catenin inhibitor ICG-001. Our findings suggest that dapagliflozin ameliorates renal fibrosis by inhibiting RIP1-RIP3-MLKL-mediated necroinflammation via Wnt3α/β-catenin/GSK-3β signaling in UUO.

scholarly journals Potential Role of the Resident Mesenchymal Stem-Like Cells in Renal Fibrogenesis after Ureteral Obstruction

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Yong-Hua Peng ◽  
Jie Xiao ◽  
Chen Yan ◽  
Lan Luo ◽  
Tao-Sheng Li
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ureteral Obstruction ◽  
Potential Role ◽  
Histological Analysis ◽  
Left Kidney ◽  
Inflammatory Cells ◽  
Unilateral Ureteral Obstruction ◽  
Renal Fibrogenesis

The mechanisms of renal fibrogenesis after ureteral obstruction remain unclear. We tried to primarily expand mesenchymal stem cells from renal tissues and then investigated their role in fibrogenesis after ureteral obstruction. Unilateral ureteral obstruction was induced by ligating the left ureteral duct of adult C57BL/6 mice. We collected the kidneys for experiments at 2, 7, and 14 days after operation. Histological analysis showed obviously fibrotic changes in the left kidney at 7 days and further increased at 14 days after ureteral obstruction. To expand mesenchymal stem cells, we minced the renal tissues into small explants (about 1 mm3) and cultured onto 10 cm dishes. Interestingly, the outgrowth of cells was observed significantly earlier from the explants of the obstructed left kidney than that of the unobstructed right kidney. These expanded cells showed the potency of adipogenic, osteogenic, and chondrogenic differentiations and positively expressed with CD44 and partly expressed with CD90, CD105, and CD106, but negatively expressed with CD34, CD45, and FSP1, suggesting the phenotype of mesenchymal stem-like cells (MSLCs). The mouse fibrosis RT2 profiler PCR array showed that many genes were changed over 2-fold in the MSLCs expanded from both kidneys at 2, 7, and 14 days after operation. Interestingly, profibrotic genes were prevalently enhanced in the left kidney with ureteral obstruction. Histological analysis also showed obviously infiltration of inflammatory cells in the left kidney at 14 days after operation. Our data indicate the potential role of resident MSLCs in renal fibrogenesis after ureteral obstruction, but further experiments are required to understand the relevant mechanisms.

scholarly journals Correction: Role of the high-affinity leukotriene B4 receptor signaling in fibrosis after unilateral ureteral obstruction in mice

PLoS ONE ◽  
2019 ◽  
Vol 14 (4) ◽  
pp. e0215625
Author(s):  
Mariko Kamata ◽  
Hideki Amano ◽  
Yoshiya Ito ◽  
Tomoe Fujita ◽  
Fumisato Otaka ◽  
...  
Keyword(s):  
Ureteral Obstruction ◽  
Leukotriene B4 ◽  
Unilateral Ureteral Obstruction ◽  
Receptor Signaling ◽  
High Affinity ◽  
Leukotriene B4 Receptor

Uptake of Pi in brush border vesicles after release of unilateral ureteral obstruction

1982 ◽  
Vol 243 (1) ◽  
pp. F29-F35
Author(s):  
S. Weinreb ◽  
K. A. Hruska ◽  
S. Klahr ◽  
M. R. Hammerman
Keyword(s):  
Brush Border ◽  
Ureteral Obstruction ◽  
Membrane Vesicles ◽  
Fractional Excretion ◽  
Unilateral Ureteral Obstruction ◽  
Renal Tubular Cells ◽  
Pi Transport ◽  
Renal Tubular ◽  
And Control

After release of complete unilateral ureteral obstruction, a decreased fractional excretion of phosphate (Pi) is observed in the postobstructed kidney compared with the nonobstructed (control) kidney. To determine whether this decrease in the urinary excretion of Pi is due to changes in Na+-dependent Pi transport across the renal brush border membranes of postobstructed and control kidneys, membrane vesicles were prepared from the brush borders of kidneys from dogs that had undergone complete unilateral ureteral obstruction. Alkaline phosphatase activity was decreased in membrane vesicles isolated from postobstructed kidneys. No differences were observed in Na+-dependent Pi transport or in Na+ uptake in membrane vesicles isolated from postobstructed as compared with control kidneys. The in vivo administration of parathyroid hormone decreased Na+-dependent Pi transport in membrane vesicles isolated from postobstructed and control kidneys despite the absence of a phosphaturic response. Our findings suggest that no intrinsic change in the transport characteristics of Pi across the luminal membrane of renal tubular cells occurs with unilateral ureteral obstruction. The findings are consistent with the suggestion that the low fractional excretion of Pi in the postobstructed kidney results from very low filtered loads of Pi on the postobstructed side.

scholarly journals Long-term renal effects of unilateral ureteral obstruction and the role of endothelin

2000 ◽  
Vol 58 (1) ◽  
pp. 242-250 ◽  
Author(s):  
Fayez T. Hammad ◽  
Antony M. Wheatley ◽  
Gerard Davis
Keyword(s):  
Ureteral Obstruction ◽  
Unilateral Ureteral Obstruction ◽  
Renal Effects
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