scholarly journals Identification of Cellular Membrane Proteins Interacting with Hepatitis B Surface Antigen using Yeast Split-Ubiquitin System

2005 ◽  
pp. 114-117 ◽  
Author(s):  
Qi Chun Toh ◽  
Tuan Lin Tan ◽  
Wei Qiang Teo ◽  
Chin Yee Ho ◽  
Subhajeet Parida ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Cellular Membrane ◽  
Ubiquitin System ◽  
Split Ubiquitin

Multiple topogenic sequences determine the transmembrane orientation of the hepatitis B surface antigen

1987 ◽  
Vol 7 (10) ◽  
pp. 3591-3601
Author(s):  
B E Eble ◽  
D R MacRae ◽  
V R Lingappa ◽  
D Ganem
Keyword(s):  
Membrane Proteins ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Signal Sequence ◽  
Hepatitis B Surface Antigen ◽  
Membrane Topology ◽  
Membrane Bilayer ◽  
Complex Membrane ◽  
Internal Sequence ◽  
Complex Protein

To investigate the mechanism by which complex membrane proteins achieve their correct transmembrane orientation, we examined in detail the hepatitis B surface antigen for sequences which determine its membrane topology. The results demonstrated the presence of at least two kinds of topogenic elements: an N-terminal uncleaved signal sequence and an internal element containing both signal and stop-transfer function. Fusion of reporter groups to either end of the protein suggested that both termini are translocated across the membrane bilayer. We propose that this topology is generated by the conjoint action of both elements and involves a specifically oriented membrane insertion event mediated by the internal sequence. The functional properties of each element can be instructively compared with those of simpler membrane proteins and may provide insight into the generation of other complex protein topologies.

scholarly journals Multiple topogenic sequences determine the transmembrane orientation of the hepatitis B surface antigen.

1987 ◽  
Vol 7 (10) ◽  
pp. 3591-3601 ◽  
Author(s):  
B E Eble ◽  
D R MacRae ◽  
V R Lingappa ◽  
D Ganem
Keyword(s):  
Membrane Proteins ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Signal Sequence ◽  
Hepatitis B Surface Antigen ◽  
Membrane Topology ◽  
Membrane Bilayer ◽  
Complex Membrane ◽  
Internal Sequence ◽  
Complex Protein

To investigate the mechanism by which complex membrane proteins achieve their correct transmembrane orientation, we examined in detail the hepatitis B surface antigen for sequences which determine its membrane topology. The results demonstrated the presence of at least two kinds of topogenic elements: an N-terminal uncleaved signal sequence and an internal element containing both signal and stop-transfer function. Fusion of reporter groups to either end of the protein suggested that both termini are translocated across the membrane bilayer. We propose that this topology is generated by the conjoint action of both elements and involves a specifically oriented membrane insertion event mediated by the internal sequence. The functional properties of each element can be instructively compared with those of simpler membrane proteins and may provide insight into the generation of other complex protein topologies.

IL10 Promotorpolymorphismen beeinflussen die Hepatitis B Surface Antigen und die Hepatitis A spezifische Immunantwort

2015 ◽  
Vol 41 (08) ◽  
Author(s):  
E Reuss ◽  
N Evers ◽  
N Dietrich ◽  
J Vollmar ◽  
PM Schneider ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis A ◽  
Hepatitis B Surface Antigen

Effects of Vancomycin on Platelets, Plasma Proteins and Hepatitis B Surface Antigen

1975 ◽  
Vol 34 (01) ◽  
pp. 083-093 ◽  
Author(s):  
Barry S Coller ◽  
W. B Lundberg ◽  
Harvey R Gralnick
Keyword(s):  
Hepatitis B ◽  
Factor Viii ◽  
Surface Antigen ◽  
Plasma Proteins ◽  
Hepatitis B Surface Antigen ◽  
Infusion Site ◽  
Vancomycin Therapy ◽  
Intravenous Injections ◽  
Low Concentrations ◽  
High Doses

SummaryThe antibiotic vancomycin shares many similarities with ristocetin, an agent noted for its effects on platelets and plasma fibrinogen. Vancomycin did not aggregate platelets as ristocetin, but platelets were incorporated into precipitates induced by vancomycin. Fibrinogen and factor VIII were precipitated from plasma at low concentrations of vancomycin. The precipitated fibrinogen remained clottable. Hepatitis B surface antigen was selectively precipitated from serum and could be recovered from the precipitate. Rabbits receiving bolus intravenous injections of high doses of vancomycin developed hypofibrinogenemia and thrombocytopenia within minutes and often went on to die. Studies with 125I-vancomycin revealed little stable binding of the antibiotic to platelets or fibrinogen. A relationship is suggested between the potent protein precipitating effects and phlebitis at the infusion site commonly associated with vancomycin therapy.

Evaluation Of The Lateral Flow Immunoassays And Electrochemiluminescent Technique For Detection Of Hepatitis B Surface Antigen

2019 ◽  
pp. 1
Author(s):  
عيظة حميد ◽  
رامى ابن مرضاح ◽  
ريم باوزير ◽  
أحمد بايعشوت ◽  
محمد العكبرى
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Lateral Flow

Hepatitis B Surface Antigen Seroprevalence Among Pre- and Post-Vaccine Cohorts in Cambodia, 2017

2018 ◽  
Author(s):  
Ork Vichit ◽  
Joseph Woodring ◽  
Md. Shafiqul Hossai ◽  
Annemarie Wasley ◽  
Shintaro Nagashima ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen
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