scholarly journals Post-Translational Modifications of PCNA in Control of DNA Synthesis and DNA Damage Tolerance-the Implications in Carcinogenesis

2021 ◽  
Vol 17 (14) ◽  
pp. 4047-4059
Author(s):  
Siyi Zhang ◽  
Tingting Zhou ◽  
Zhuo Wang ◽  
Fei Yi ◽  
Chunlu Li ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Synthesis ◽  
Damage Tolerance ◽  
Dna Damage Tolerance ◽  
Post Translational Modifications

scholarly journals Replication intermediate architecture reveals the chronology of DNA damage tolerance pathways at UV-stalled replication forks in human cells

2020 ◽  
Author(s):  
Yann Benureau ◽  
Caroline Pouvelle ◽  
Eliana Moreira Tavares ◽  
Pauline Dupaigne ◽  
Emmanuelle Despras ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Synthesis ◽  
Replication Fork ◽  
Damage Tolerance ◽  
Dna Lesions ◽  
Human Cells ◽  
Compensatory Mechanism ◽  
Dna Damage Tolerance ◽  
Replication Intermediate

AbstractDNA lesions in S phase threaten genome stability. The DNA damage tolerance (DDT) pathways overcome these obstacles and allow completion of DNA synthesis by the use of specialised translesion (TLS) DNA polymerases or through recombination-related processes. However, how these mechanisms coordinate with each other and with bulk replication remain elusive. To address these issues, we monitored the variation of replication intermediate architecture in response to ultraviolet irradiation using transmission electron microscopy. We show that the TLS polymerase η, able to accurately bypass the major UV lesion and mutated in the skin cancer-prone xeroderma pigmentosum variant (XPV) syndrome, acts at the replication fork to resolve uncoupling and prevent post-replicative gap accumulation. Repriming occurs as a compensatory mechanism when this on-the-fly mechanism cannot operate, and is therefore predominant in XPV cells. Interestingly, our data support a recombination-independent function of RAD51 at the replication fork to sustain repriming. Finally, we provide evidence for the post-replicative commitment of recombination in gap repair and for pioneering observations of in vivo recombination intermediates. Altogether, we propose a chronology of UV damage tolerance in human cells that highlights the key role of polη in shaping this response and ensuring the continuity of DNA synthesis.

scholarly journals PDIP38/PolDIP2 controls the DNA damage tolerance pathways by increasing the relative usage of translesion DNA synthesis over template switching

PLoS ONE ◽  
2019 ◽  
Vol 14 (3) ◽  
pp. e0213383 ◽  
Author(s):  
Masataka Tsuda ◽  
Saki Ogawa ◽  
Masato Ooka ◽  
Kaori Kobayashi ◽  
Kouji Hirota ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Synthesis ◽  
Damage Tolerance ◽  
Template Switching ◽  
Dna Damage Tolerance ◽  
Translesion Dna Synthesis ◽  
Translesion Dna

scholarly journals Identification of novel DNA-damage tolerance genes reveals regulation of translesion DNA synthesis by nucleophosmin

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Omer Ziv ◽  
Amit Zeisel ◽  
Nataly Mirlas-Neisberg ◽  
Umakanta Swain ◽  
Reinat Nevo ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Synthesis ◽  
Damage Tolerance ◽  
Dna Damage Tolerance ◽  
Translesion Dna Synthesis ◽  
Translesion Dna

scholarly journals Novel conserved motifs in Rev1 C-terminus are required for mutagenic DNA damage tolerance

DNA Repair ◽  
2008 ◽  
Vol 7 (9) ◽  
pp. 1455-1470 ◽  
Author(s):  
Sanjay D'Souza ◽  
Lauren S. Waters ◽  
Graham C. Walker
Keyword(s):  
Dna Damage ◽  
Damage Tolerance ◽  
Dna Damage Tolerance ◽  
Conserved Motifs ◽  
C Terminus

scholarly journals DNA-damage tolerance mediated by PCNA•Ub fusions in human cells is dependent on Rev1 but not Polη

2013 ◽  
Vol 41 (15) ◽  
pp. 7356-7369 ◽  
Author(s):  
Zhoushuai Qin ◽  
Mengxue Lu ◽  
Xin Xu ◽  
Michelle Hanna ◽  
Naoko Shiomi ◽  
...  
Keyword(s):  
Dna Damage ◽  
Damage Tolerance ◽  
Human Cells ◽  
Dna Damage Tolerance
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