scholarly journals MicroRNA-322 Regulates Self-renewal of Mouse Spermatogonial Stem Cells through Rassf8

2019 ◽  
Vol 15 (4) ◽  
pp. 857-869 ◽  
Author(s):  
Yinjuan Wang ◽  
Xiaoyong Li ◽  
Xiaowen Gong ◽  
Yongqiang Zhao ◽  
Ji Wu
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
Self Renewal

Both Gfrα1a and Gfrα1b Are Involved in the Self-Renewal and Maintenance of Spermatogonial Stem Cells in Medaka

2018 ◽  
Vol 27 (23) ◽  
pp. 1658-1670
Author(s):  
Yang Zhao ◽  
Zhuo Yang ◽  
Yuan Wang ◽  
Yubing Luo ◽  
Fan Da ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
The Self ◽  
Self Renewal

PD08-03 PROFILING OF HUMAN ADULT SPERMATOGONIAL STEM CELLS REVEALS TRANSCRIPTION AND SIGNALING PROGRAMS FOR SELF RENEWAL AND DIFFERENTIATION

2017 ◽  
Vol 197 (4S) ◽  
Author(s):  
Jingtao Guo ◽  
Edward J. Grow ◽  
Chongil Yi ◽  
Douglas T. Carrell ◽  
James M. Hotaling ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
Self Renewal ◽  
Human Adult

scholarly journals Expression and functional analyses of ephrin type-A receptor 2 in mouse spermatogonial stem cells†

2019 ◽  
Vol 102 (1) ◽  
pp. 220-232 ◽  
Author(s):  
Hiroko Morimoto ◽  
Mito Kanatsu-Shinohara ◽  
Kyle E Orwig ◽  
Takashi Shinohara
Keyword(s):  
Stem Cells ◽  
Cell Sorting ◽  
Type A ◽  
Spermatogonial Stem Cells ◽  
Fibroblast Growth Factor 2 ◽  
Self Renewal ◽  
Spermatogonial Transplantation ◽  
Short Hairpin ◽  
Functional Analyses ◽  
Magnetic Activated Cell Sorting

Abstract Spermatogonial stem cells (SSCs) undergo continuous self-renewal division in response to self-renewal factors. The present study identified ephrin type-A receptor 2 (EPHA2) on mouse SSCs and showed that supplementation of glial cell-derived neurotrophic factor (GDNF) and fibroblast growth factor 2 (FGF2), which are both SSC self-renewal factors, induced EPHA2 expression in cultured SSCs. Spermatogonial transplantation combined with magnetic-activated cell sorting or fluorescence-activated cell sorting also revealed that EPHA2 was expressed in SSCs. Additionally, ret proto-oncogene (RET) phosphorylation levels decreased following the knockdown (KD) of Epha2 expression via short hairpin ribonucleic acid (RNA). Although the present immunoprecipitation experiments did not reveal an association between RET with EPHA2, RET interacted with FGFR2. The Epha2 KD decreased the proliferation of cultured SSCs and inhibited the binding of cultured SSCs to laminin-coated plates. The Epha2 KD also significantly reduced the colonization of testis cells by spermatogonial transplantation. EPHA2 was also expressed in human GDNF family receptor alpha 1-positive spermatogonia. The present results indicate that SSCs express EPHA2 and suggest that it is a critical modifier of self-renewal signals in SSCs.

Construction and analysis of a protein–protein interaction network related to self-renewal of mouse spermatogonial stem cells

2015 ◽  
Vol 11 (3) ◽  
pp. 835-843 ◽  
Author(s):  
Wenhai Xie ◽  
Jin Sun ◽  
Ji Wu
Keyword(s):  
Stem Cells ◽  
Protein Interaction ◽  
Protein Interaction Network ◽  
Interaction Network ◽  
Spermatogonial Stem Cells ◽  
Self Renewal ◽  
Protein Protein Interaction ◽  
Reproductive Life ◽  
Protein Protein Interaction Network

Spermatogonial stem cells (SSCs) are responsible for sustained spermatogenesis throughout the reproductive life of the male.

scholarly journals Bioinformatics Analysis of Transcriptomic Data Reveals Refined Functional Networks for the Self-Renewal of Mouse Spermatogonial Stem Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Min Wang ◽  
Wene Zhao ◽  
Fuqiang Wang ◽  
Xiufeng Ling ◽  
Daozhen Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Signaling Pathways ◽  
Bioinformatics Analysis ◽  
Cell Types ◽  
Spermatogonial Stem Cells ◽  
Expression Index ◽  
Extrinsic Factors ◽  
Self Renewal ◽  
Proliferation And Differentiation ◽  
Disease Associations

Spermatogonial stem cells (SSCs) are exquisitely regulated to reach a balance between proliferation and differentiation in the niche of seminiferous epithelium. Several extrinsic factors such as GDNF are reported to switch the transition, activating various intrinsic signaling pathways. Transcriptomics analysis could provide a comprehensive landscape of gene expression and regulation. Here, we reanalyzed a previously published transcriptome of two cell types (standing for self-renewing and differentiating SSCs correspondingly). First, we proposed a new parameter, the expression index, to sort the genes considering both absolute and relative expression levels. Using a dynamic statistical model, we identified a list of 1119 candidate genes for SSC self-renewal with the best enrichment of canonical markers. Finally, based on interaction relations, we further optimized the list and constructed a refined network containing integrated information of interactions, expression alternations, biological functions, and disease associations. Further annotation of the 521 refined genes involved in the network revealed an enrichment of well-studied signaling pathways. We believe that the refined network could help us better understand the regulation of SSCs’ fates, as well as find novel regulators or targets for SSC self-renewal or preservation of male fertility.

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