scholarly journals Inhibition of Autophagy with Chloroquine Enhanced Sinoporphyrin Sodium Mediated Photodynamic Therapy-induced Apoptosis in Human Colorectal Cancer Cells

2019 ◽  
Vol 15 (1) ◽  
pp. 12-23 ◽  
Author(s):  
Bing Zhu ◽  
Shanxin Li ◽  
Lei Yu ◽  
Wei Hu ◽  
Dandan Sheng ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Human Colorectal Cancer ◽  
Colorectal Cancer Cells ◽  
Induced Apoptosis ◽  
Human Colorectal Cancer Cells

scholarly journals miR-214 sensitizes human colorectal cancer cells to doxorubicin by p53 targeting

2020 ◽  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Human Colorectal Cancer ◽  
P53 Expression ◽  
Colorectal Cancer Cells ◽  
Scratch Wound ◽  
And Migration ◽  
Induced Apoptosis ◽  
Ht 29 ◽  
Human Colorectal Cancer Cells

Objectives: This study aimed to investigate the potential function of miR-214 in the apoptosis induction by targeting p53 in human colorectal cancer cells (CRC) in combination with doxorubicin (DOX). Methods: miR-214 mimics were transfected to HT-29 CRC cells. Following that, the transfected cells were treated with DOX. Cell viability, apoptosis, and migration were evaluated by MTT, flow cytometry, and scratch-wound motility assays, respectively. Furthermore, the expression level of miR-214 and p53 was evaluated by qRT-PCR. Results: miR-214 transfection significantly inhibited the cell proliferation rate (P<0.05), induced apoptosis (P<0.05), and harnessed migration (P<0.05) in the HT-29 cells after 48 h. Furthermore, more effectiveness was observed in combination with DOX. Additionally, miR-214 transfection led to a reduction in p53 expression offering that it might be a potential target for miR-214. Conclusion: In conclusion, miR-214 sensitizes HT-29 cells to doxorubicin by targeting p53 indicating the significant role of this miRNA in colorectal cancer chemotherapy.

Effects of CLIC4 on Fucoxanthinol-Induced Apoptosis in Human Colorectal Cancer Cells

2020 ◽  
pp. 1-10
Author(s):  
Reo Yokoyama ◽  
Hiroyuki Kojima ◽  
Rie Takai ◽  
Tohru Ohta ◽  
Hayato Maeda ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Human Colorectal Cancer ◽  
Colorectal Cancer Cells ◽  
Induced Apoptosis ◽  
Human Colorectal Cancer Cells

scholarly journals Naja ashei venom induces mitochondria-mediated apoptosis in human colorectal cancer cells

2019 ◽  
Author(s):  
Natalia Rozman Antolikova ◽  
Martin Kello ◽  
Martina Zigova ◽  
Viera Tischlerova ◽  
Vladimir Petrilla ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Flow Cytometric Analysis ◽  
Annexin V ◽  
Parp Cleavage ◽  
Human Colorectal Cancer ◽  
Colorectal Cancer Cells ◽  
Apoptotic Effect ◽  
Induced Apoptosis ◽  
Human Colorectal Cancer Cells

In the present study, we investigated the antiproliferative activity of Naja ashei full venom (NAV) on human colorectal cancer cells. The NAV-induced antiproliferative effect was associated with cell cycle arrest in S phase and increased number of cells with sub G0/G1 DNA content, which is considered a marker of apoptosis. Apoptosis has also been confirmed with annexin V/PI staining. Furthermore, flow cytometric analysis revealed loss of mitochondrial membrane potential with concomitant increase in cytochrome c and Smac/DIABLO protein content. These effects were associated with the activation of caspase-9 and caspase-3, as well as with PARP cleavage. Moreover, phosphorylation of antiapoptotic Bcl-2 protein in NAV-treated HCT116 was observed. In conclusion, our study for the first time documented antiproliferative/pro-apoptotic effect of NAV in colorectal cancer cells. Our results strongly suggest the involvement of mitochondria in NAV induced apoptosis of cancer cells. Future studies are needed to further examine the potential of NAV in the treatment of colon cancer.

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