scholarly journals Simultaneously targeting DNA damage repair pathway and mTORC1/2 results in small cell lung cancer growth arrest via ER stress-induced apoptosis

2018 ◽  
Vol 14 (10) ◽  
pp. 1221-1231 ◽  
Author(s):  
Bin Fang ◽  
Aarthi Kannan ◽  
Tao Guo ◽  
Ling Gao
Keyword(s):  
Lung Cancer ◽  
Dna Damage ◽  
Small Cell Lung Cancer ◽  
Growth Arrest ◽  
Small Cell ◽  
Cancer Growth ◽  
Repair Pathway ◽  
Small Cell Lung ◽  
Damage Repair ◽  
Induced Apoptosis

MMP10 involved in Radiosensitivity in Non-Small Cell Lung Cancer via DNA damage repair pathway

2020 ◽  
Author(s):  
Xiao Lei ◽  
Yuanyuan Chen ◽  
Na Ma ◽  
Pei Zhang ◽  
Yanan Han ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Dna Damage ◽  
Small Cell Lung Cancer ◽  
Clonogenic Assay ◽  
Dna Damage Repair ◽  
Small Cell ◽  
Repair Pathway ◽  
Small Cell Lung ◽  
Damage Repair ◽  
Apoptosis Assay

Abstract Background Non-small cell lung cancer (NSCLC) is an aggressive subtype of lung cancer with high mortality and morbidity. Our purpose in this study is to investigate the effect of MMP10 in NSCLC during radiotherapy and its mechanism. Materials and methods We analyzed MMP10 expression and overall survival rate of patients via UALCAN analysis. Western blot analysis was used to test the protein expression. We applied clonogenic assay and apoptosis assay to detect radioresistance in vitro. The level of DNA damage and the DNA damage repair were disclosed via comet assay and γH2AX foci assay. Results Our results showed that high MMP10 expression in Lung Adenocarcinoma specimens is associated with poor outcomes of patients. Knockdown MMP10 induced radiosensitization in A549 cells via clonogenic assay and apoptosis assay. Furthermore, we found MMP10 siRNA enhanced DNA damage of NSCLC cells and MMP10 involved in DNA damage repair after IR. Conclusion Our data highlighted that MMP10 plays a crucial role in NSCLC after radiation via DNA damage repair pathway, and the regulating effect of MMP10 on radiosensitivity in NSCLC might confer to therapeutic implications to NSCLC radiotherapy.

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