scholarly journals Generation of Mesenchymal Stem Cells from Human Embryonic Stem Cells in a Complete Serum-free Condition

2018 ◽  
Vol 14 (13) ◽  
pp. 1901-1909 ◽  
Author(s):  
Enqin Li ◽  
Zhenwu Zhang ◽  
Bin Jiang ◽  
Li Yan ◽  
Jung Woo Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Serum Free ◽  
Free Condition ◽  
Serum Free Condition

Cryopreservation of Human Embryonic Stem Cells Derived-Cardiomyocytes Induced by BMP2 in Serum-Free Condition

2011 ◽  
Vol 18 (3) ◽  
pp. 252-260 ◽  
Author(s):  
Yoon Young Kim ◽  
Seung-Yup Ku ◽  
Hung-Ching Liu ◽  
Hyun-Jai Cho ◽  
Sun Kyung Oh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Serum Free ◽  
Free Condition ◽  
Serum Free Condition

Derivation of embryonic stem cells from Kunming mice IVF blastocyst in feeder- and serum-free condition

2015 ◽  
Vol 51 (6) ◽  
pp. 541-545 ◽  
Author(s):  
Xiaokun Liu ◽  
Qiang Wei ◽  
Junhong Zhang ◽  
Wanli Yang ◽  
Xiaoe Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Serum Free ◽  
Free Condition ◽  
Serum Free Condition

scholarly journals Differentiation of Human Embryonic Stem Cells into Engrafting Myogenic Precursor Cells

2019 ◽  
Vol 1 (1) ◽  
pp. 01-05
Author(s):  
Stalin Reddy Challa ◽  
Swathi Goli
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Precursor Cells ◽  
Functional Improvement ◽  
Serum Free ◽  
Myogenic Stem Cells ◽  
Serum Free Conditions ◽  
Myogenic Precursor

Degenerative muscle diseases affect muscle tissue integrity and function. Human embryonic stem cells (hESC) are an attractive source of cells to use in regenerative therapies due to their unlimited capacity to divide and ability to specialize into a wide variety of cell types. A practical way to derive therapeutic myogenic stem cells from hESC is lacking. In this study, we demonstrate the development of two serum-free conditions to direct the differentiation of hESC towards a myogenic precursor state. Using TGFß and PI3Kinase inhibitors in combination with bFGF we showed that one week of differentiation is sufficient for hESC to specialize into PAX3+/PAX7+ myogenic precursor cells. These cells also possess the capacity to further differentiate in vitro into more specialized myogenic cells that express MYOD, Myogenin, Desmin and MYHC, and showed engraftment in vivo upon transplantation in immunodeficient mice. Ex vivo myomechanical studies of dystrophic mouse hindlimb muscle showed functional improvement one month post-transplantation. In summary, this study describes a promising system to derive engrafting muscle precursor cells solely using chemical substances in serum-free conditions and without genetic manipulation.

Expansion of human embryonic stem cells in defined serum‐free medium devoid of animal‐derived products

2005 ◽  
Vol 91 (6) ◽  
pp. 688-698 ◽  
Author(s):  
Yan Li ◽  
Sandra Powell ◽  
Elisa Brunette ◽  
Jane Lebkowski ◽  
Ramkumar Mandalam
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free
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