scholarly journals Low-intensity pulsed ultrasound promotes Schwann cell viability and proliferation via the GSK-3β/β-catenin signaling pathway

2018 ◽  
Vol 14 (5) ◽  
pp. 497-507 ◽  
Author(s):  
Cong Ren ◽  
Xiaohui Chen ◽  
Ning Du ◽  
Shuo Geng ◽  
Yingying Hu ◽  
...  
Keyword(s):  
Schwann Cell ◽  
Cell Viability ◽  
Signaling Pathway ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Gsk 3Β

scholarly journals Effects of Low-Intensity Pulsed Ultrasound on the Proliferation of Human Umbilical Cord Mesenchymal Stem Cells and Their Chondrogenic Differentiation via Bmp2 /Smad Signaling Pathway

2020 ◽  
Author(s):  
Xuhong JIN ◽  
Hejie Wang ◽  
Jiaju Che ◽  
Xiafu Chen ◽  
Dengao Huang ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Chondrogenic Differentiation ◽  
Blue Staining ◽  
Control Group ◽  
Human Umbilical Cord ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Proliferation Activity ◽  
Smad Signaling Pathway

Abstract BackgroundAlthough Low-intensity pulsed ultrasound (LIPUS) regiment has been applied to the treatment of cartilage injury clinically, the effect of LIPUS stress loading on the proliferation and differentiation of hUCMSCs is still controversial. Yet the specific mechanical mechanism of LIPUS stimulates cartilage differentiation of hUCMSCs has not been clarified.ObjectiveTo investigate the effects of low intensity pulsed ultrasound (LIPUS) on proliferation and chondrogenic differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs) via BMP2 /smad signaling pathway in vitro.MethodThe hUCMSCs at P4 were randomly divided into control group(no LIPUS), L30 group (30mW/cm2), L50 group (50mW/cm2), L80 group (80mW/cm2), each LIPUS group respectively loaded 5, 10, 20 min/d, the cell proliferation activity was detected by CCK-8 assay after continuous irradiation for 5 days. For chondrogenic differentiation, hUCMSCs were divided 4 groups as follows: control group, Noggin group, LIPUS group, Noggin+LIPUS group, LIPUS irradiation was given 50w/cm2, 5min/d. Cartilage formation after 3 weeks was evaluated by Alician blue staining for GAG and COL II immunohistochemical staining. Furthermore, RT-PCR analysis of Sox-9, COL II Aggrecan, BMP2, Smad1, Smad5 and Smad9. Result： (1) The proliferation activity of L50 group(50w/cm2 , 5min/d) was noticeably higher compared with other groups, Alcian blue staining of GAG , COL II fluorescent staining in the LIPUS group were much stronger, quantitative RT-PCR assays showed that the expression of COL II, GAG, Sox-9, smad1, smad5 and smad9 in LIPUS group was significantly increased. Conclusion：The suitable LIPUS irradiation can promote cell proliferation, 50mW/cm2 intensity at 5min/d is most significant. And it can promote chondrogenesis of hUCMSCs via upregulating MP2 /smads signaling pathway.

Effect of Low-Intensity Pulsed Ultrasound (LIPUS) on Endochondral Ossification via the Wnt Signaling Pathway

2017 ◽  
Vol 31 (7) ◽  
pp. S4-S5 ◽  
Author(s):  
Ken Kumagai ◽  
Yasuteru Yamaguchi ◽  
Kazuma Miyatake ◽  
Hironori Tanabe ◽  
Sosuke Imai ◽  
...  
Keyword(s):  
Wnt Signaling ◽  
Signaling Pathway ◽  
Endochondral Ossification ◽  
Wnt Signaling Pathway ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

scholarly journals Low-Intensity Pulsed Ultrasound Effect on MIO-M1 Cell Viability: Setup Validation and Standing Waves Analysis

2020 ◽  
Vol 11 (1) ◽  
pp. 271
Author(s):  
Irais Poblete-Naredo ◽  
Mario Ibrahin Gutierrez ◽  
Diana Estela Mendoza-Sánchez ◽  
Arturo Ortega ◽  
Arnulfo Albores ◽  
...  
Keyword(s):  
Cell Viability ◽  
Treatment Duration ◽  
Standing Waves ◽  
Cell Models ◽  
Pulsed Ultrasound ◽  
Culture Dish ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Cell Mortality ◽  
Ultrasound Effect

Low-intensity pulsed ultrasound (LIPUS) has been proposed for novel therapies still under study, where similar parameters and protocols have been used for producing opposite effects that range from increasing cell viability to provoking cell death. Those divergent outcomes make the generalization of expected effects difficult for cell models not yet studied. This paper presents the effect of LIPUS on the viability of the MIO-M1 cell line for two well-established setups and different protocols; the acoustic intensities, duty factors, and treatment duration were varied. Measurements and models for acoustic and thermal analysis are included for proposing a solution to improve the reproducibility of this kind of experiments. Results indicate that MIO-M1 viability is less affected for the cells treated through a dish that is partially immersed in water; in these conditions, the cells neither show detrimental nor proliferative effects at intensities lower than 0.4 W/cm2 at 20% duty factor. However, cell viability was reduced when LIPUS was followed by cell subculturing. Treating the cells through a gel, with the culture dish placed on the transducer, increases cell mortality by the production of standing waves and mixed vibration-acoustical effects. Using the water-based setup with a 1° dish inclination reduces the effects of standing waves.

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