scholarly journals Insights into the Nanog gene: A propeller for stemness in primitive stem cells

2016 ◽  
Vol 12 (11) ◽  
pp. 1372-1381 ◽  
Author(s):  
Wei Zhang ◽  
Yi Sui ◽  
Jun Ni ◽  
Tao Yang
Keyword(s):  
Stem Cells ◽  
Nanog Gene

scholarly journals Epigenetic regulation of Nanog gene in embryonic stem and trophoblast stem cells

2007 ◽  
Vol 12 (3) ◽  
pp. 387-396 ◽  
Author(s):  
Naoko Hattori ◽  
Yuko Imao ◽  
Koichiro Nishino ◽  
Naka Hattori ◽  
Jun Ohgane ◽  
...  
Keyword(s):  
Stem Cells ◽  
Epigenetic Regulation ◽  
Embryonic Stem ◽  
Trophoblast Stem Cells ◽  
Trophoblast Stem ◽  
Nanog Gene

scholarly journals Repression of Nanog Gene Transcription by Tcf3 Limits Embryonic Stem Cell Self-Renewal

2006 ◽  
Vol 26 (20) ◽  
pp. 7479-7491 ◽  
Author(s):  
Laura Pereira ◽  
Fei Yi ◽  
Bradley J. Merrill
Keyword(s):  
Stem Cells ◽  
Transcription Factors ◽  
Molecular Mechanisms ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Regulatory Region ◽  
Embryonic Stem ◽  
Dual Function ◽  
Self Renewal ◽  
Nanog Gene

ABSTRACT The dual function of stem cells requires them not only to form new stem cells through self-renewal but also to form lineage-committed cells through differentiation. Embryonic stem cells (ESC), which are derived from the blastocyst inner cell mass, retain properties of self-renewal and the potential for lineage commitment. To balance self-renewal and differentiation, ESC must carefully control the levels of several transcription factors, including Nanog, Sox2, and Oct4. While molecular mechanisms promoting transcription of these genes have been described, mechanisms preventing excessive levels in self-renewing ESC remain unknown. By examining the function of the TCF family of transcription factors in ESC, we have found that Tcf3 is necessary to limit the steady-state levels of Nanog mRNA, protein, and promoter activity in self-renewing ESC. Chromatin immunoprecipitation and promoter reporter assays showed that Tcf3 bound to a promoter regulatory region of the Nanog gene and repressed its transcriptional activity in ESC through a Groucho interaction domain-dependent process. The absence of Tcf3 caused delayed differentiation of ESC in vitro as elevated Nanog levels persisted through 5 days of embryoid body formation. These new data support a model wherein Tcf3-mediated control of Nanog levels allows stem cells to balance the creation of lineage-committed and undifferentiated cells.

scholarly journals Relation between Immune cell response and stemness genes expression in breast cancer; A new approach in NANOG gene and Let7-a expression in breast cancer cell lines

2020 ◽  
Vol 6 (2) ◽  
pp. e21-e21
Author(s):  
Zeynab Aliyari-Serej ◽  
Ayyub Ebrahimi ◽  
Tohid Kazemi ◽  
Souzan Najafi ◽  
Elmira Roshani ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Immune Cells ◽  
Immune Cell ◽  
Preclinical Study ◽  
Breast Cancer Cell Lines ◽  
Developmental Behavior ◽  
Genes Expression ◽  
Nanog Gene ◽  
Immune Cell Response

Introduction: Failure and recurrence in breast cancer treatment cause a great obstacle in cancer therapy and identification of cell population named cancer stem cells (CSCs) in the tumor can be led us to define it as target in novel therapeutic strategy. Objectives: The aim of this study is the finding of correlation between stemness and metastatic characteristic, also knowing CSCs as a potential target of therapy because of its developmental behavior and similarities with normal stem cells. Materials and Methods: Here, we focus on the expression of NANOG in breast CSCs, a key molecule in the physiological process of stem cells and the Let-7a that is involved in the differentiation of the cells. Results: In this work, we found that NANOG was highly expressed in SKBR3 and down-regulation of let-7a, as a differentiation miRNA, was found in MDA-MB-468 cells. Conclusion: It will be critical for the developing of effective anti-tumor drugs, utilizing mentioned concepts. Inhibition of NANOG in combination with Let-7a up-regulation can help to decrease the stemness and increase the differentiation of CSCs. The decrease of stemness and increase of differentiation initiate the apoptotic process. So, modification in the mechanism of apoptosis beside anti-cancer drugs provide a good preclinical study goal. However, in order to these drugs become clinical, the problems of their side effects and toxicity must be solved. Differentiation of CSCs provides an optimal condition to activity of immune cells which never let them escape from immune cells by alteration of immunogenicity.

scholarly journals Regulation of the Nanog gene by both positive and negativecis-regulatory elements in embryonal carcinoma cells and embryonic stem cells

2009 ◽  
Vol 76 (2) ◽  
pp. 173-182 ◽  
Author(s):  
Brian Boer ◽  
Jesse L. Cox ◽  
David Claassen ◽  
Sunil Kumar Mallanna ◽  
Michelle Desler ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonal Carcinoma ◽  
Embryonic Stem ◽  
Regulatory Elements ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Nanog Gene

scholarly journals NANOG Plays a Hierarchical Role in the Transcription Network Regulating the Pluripotency and Plasticity of Adipose Tissue-Derived Stem Cells (ASCs)

2017 ◽  
Author(s):  
Maria Pitrone ◽  
Giuseppe Pizzolanti ◽  
Laura Tomasello ◽  
Antonina Coppola ◽  
Lorenzo Morini ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Markers ◽  
Transcription Network ◽  
Cell Markers ◽  
Nanog Gene ◽  
Subcutaneous Adipose

The stromal vascular cell fraction (SVF) of visceral and subcutaneous adipose tissue (VAT and SAT) has increasingly come into focus in stem cell research, since these compartments represent a rich source of multipotent adipose-derived stem cells (ASCs). ASCs exhibit a self- renewal potential and differentiation capacity. Our aim was to study the different expression of embryonic stem cell markers NANOG, SOX2 and OCT3/4 and to evaluate if there exists a hierarchal role in this network in ASCs derived from both SAT and VAT. ASCs were isolated from SAT and VAT biopsies of 72 consenting patients (23 men, 47 women; age 45 ± 10; BMI between 25 and 30 range) undergoing elective open-abdominal surgery. Sphere-forming capability was evaluated by plating cells in low adhesion plastic. Stem cell markers CD90 and CD105 were analyzed by flow cytometry and stem cell transcription factors NANOG, SOX2 and OCT3/4 were detected by immunoblotting and Real-Time PCR. NANOG, SOX2 and OCT3/4 interplay was explored by gene silencing. ASCs from VAT and SAT confirmed their mesenchymal stem cell (MSC) phenotype expressing the specific MSC markers CD90 and CD105 and NANOG, SOX2 and OCT3/4. NANOG silencing induced a significant OCT 3/4 (70% ± 0.05) and SOX2 (75% ± 0.03) down-regulation whereas SOX2 silencing did not affect NANOG gene expression. Adipose tissue is an important source of MSC, and siRNA experiments endorse a hierarchical role of NANOG in the complex transcription network that regulates pluripotency and plasticity.

scholarly journals NANOG Plays a Hierarchical Role in the Transcription Network Regulating the Pluripotency and Plasticity of Adipose Tissue-Derived Stem Cells (ASCs)

2017 ◽  
Author(s):  
Maria Pitrone ◽  
Giuseppe Pizzolanti ◽  
Laura Tomasello ◽  
Antonina Coppola ◽  
Lorenzo Morini ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Markers ◽  
Transcription Network ◽  
Cell Markers ◽  
Nanog Gene ◽  
Subcutaneous Adipose

The stromal vascular cell fraction (SVF) of visceral and subcutaneous adipose tissue (VAT and SAT) has increasingly come into focus in stem cell research, since these compartments represent a rich source of multipotent adipose-derived stem cells (ASCs). ASCs exhibit a self- renewal potential and differentiation capacity. Our aim was to study the different expression of embryonic stem cell markers NANOG, SOX2 and OCT3/4 and to evaluate if there exists a hierarchal role in this network in ASCs derived from both SAT and VAT. ASCs were isolated from SAT and VAT biopsies of 72 consenting patients (23 men, 47 women; age 45 ± 10; BMI between 25 and 30 range) undergoing elective open-abdominal surgery. Sphere-forming capability was evaluated by plating cells in low adhesion plastic. Stem cell markers CD90 and CD105 were analyzed by flow cytometry and stem cell transcription factors NANOG, SOX2 and OCT3/4 were detected by immunoblotting and Real-Time PCR. NANOG, SOX2 and OCT3/4 interplay was explored by gene silencing. ASCs from VAT and SAT confirmed their mesenchymal stem cell (MSC) phenotype expressing the specific MSC markers CD90, CD105, NANOG, SOX2 and OCT3/4. NANOG silencing induced a significant OCT 3/4 (70% ± 0.05) and SOX2 (75% ± 0.03) down-regulation whereas SOX2 silencing did not affect NANOG gene expression. Adipose tissue is an important source of MSC, and siRNA experiments endorse a hierarchical role of NANOG in the complex transcription network that regulates pluripotency and plasticity.

Proliferation and multi-differentiation potentials of human mesenchymal stem cells on thermoresponsive PDMS surfaces grafted with PNIPAAm

2010 ◽  
Vol 30 (6) ◽  
pp. 455-455 ◽  
Author(s):  
Dongyan Shi ◽  
Dan Ma ◽  
Feiqing Dong ◽  
Chen Zong ◽  
Liyue Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells
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