scholarly journals Upregulation of the Non-Coding RNA OTUB1-isoform 2 Contributes to Gastric Cancer Cell Proliferation and Invasion and Predicts Poor Gastric Cancer Prognosis

2016 ◽  
Vol 12 (5) ◽  
pp. 545-557 ◽  
Author(s):  
Yi-qin Wang ◽  
Qiong-yan Zhang ◽  
Wei-wei Weng ◽  
Yong Wu ◽  
Yu-si Yang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Proliferation ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Cancer Prognosis ◽  
Cancer Cell Proliferation ◽  
Non Coding Rna ◽  
Gastric Cancer Cell Proliferation ◽  
Proliferation And Invasion

Long Non-Coding RNA Small Nucleolar RNA Host Gene 15 (SNHG15) Regulates Gastric Cancer Cell Proliferation and Invasion Through Targeting MiR-92a

2020 ◽  
Vol 10 (4) ◽  
pp. 518-524
Author(s):  
Feng Deng ◽  
Xiaoping Zhou ◽  
Yang Liu ◽  
Haiding Wang ◽  
Yinzi Li
Keyword(s):  
Gastric Cancer ◽  
Cell Proliferation ◽  
Real Time ◽  
Cancer Cell ◽  
Real Time Pcr ◽  
Gastric Cancer Cell ◽  
Cancer Cell Proliferation ◽  
Pathological Stage ◽  
Gastric Cancer Cell Proliferation ◽  
Proliferation And Invasion

Gastric cancer pathogenesis remains to be elucidated. LncRNAs involve in tumorigenesis. LncRNA SNHG15 is abnormally expressed in several tumors, but its role in gastric cancer is unclear. The tumor tissue and adjacent tissues of gastric cancer patients were collected for measuring LncRNA SNHG15 and miR-92a expressions by Real time PCR. Gastric cancer cell line SGC-7901 was divided into NC group, si-SNHG15 group, and si-UCA1+ miR-92a inhibitor group followed by analysis of cell proliferation by MTT assay, Capase-3 activity as well as Bax and Bcl-2 level by Real-time PCR. Gastric cancer tissues presented significantly increased LncRNA SNHG15 and decreased miR-92a expression than adjacent tissues (P < 0 05). LncRNA SNHG15 and miR-92a expression was associated with the tumor differentiation and pathological stage (P < 0 05) with a negative correlation between them. SNHG15 siRNA transfection significantly inhibited SNHG15 expression and tumor cell proliferation and invasion, increased Caspase 3 activity and Bax level, and decreased Bcl2 (P < 0 05). miR-92a was a targeted miRNA for LncRNA SNHG15. Addition of miR-92a inhibitor can significantly reverse the above changes (P < 0 05). LncRNA SNHG15 expression is increased and miR-92a expression is decreased in gastric cancer and correlated with the tumor pathological stage. LncRNA SNHG15 regulates human gastric cancer cell proliferation, apoptosis and invasion via targeting miR-92a.

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