scholarly journals Effect of vanadium(IV) on the growth of Artemisia tilesii “hairy” root culture

2019 ◽  
Vol 25 ◽  
pp. 277-280
Author(s):  
N. A. Matvieieva ◽  
O. A. Gavryliuk ◽  
V. P. Duplij
Keyword(s):  
Hairy Root ◽  
In Vitro Model ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Transgenic Roots ◽  
Vitro Model ◽  
Mass Increase ◽  
Plant Shoots

Aim. Determination of the effect of toxic metals on plants is of considerable interest, since contamination of the environment with toxicants is a threat to the safety of agriculture and human health. Vanadium increased content in soil leads to the suppression of plant growth. The aim of the work was to determine the peculiarities of the influence of V(IV) (neutral solution [V4+ cit]) on Artemisia tilesii Ledeb plants in the in vitro model system and to compare this effect on the growth of “hairy” root culture and plants. Methods. Plant shoots and transgenic roots were cultivated on a solidified Murashige and Skoog medium with V(IV) at 1, 5, 10, 50 and 100 mg/L. Mass increase was measured after 4 weeks. Results. It was found that V(IV) at 50 mg/L concentration completely inhibited root formation and led to shoot death. Transgenic roots survived even at 100 mg/L of vanadium. Conclusions. The results of the work demonstrated that A. tilesii “hairy” roots were characterized by greater resistance to V(IV) than control plants. In addition, differences in the sensitivity of different root lines to this compound were detected. This fact probably could be explained by the transformation, the transfer of agrobacterial genes, and changes in the secondary metabolism in transgenic roots. Keywords: vanadium(IV), Artemisia tilesii Ledeb., “hairy” root culture.

Hairy Root Culture for In Vitro Production of Secondary Metabolites: A Promising Biotechnological Approach

2018 ◽  
pp. 235-250
Author(s):  
Ravi Shankar Singh ◽  
Tirthartha Chattopadhyay ◽  
Dharamsheela Thakur ◽  
Nitish Kumar ◽  
Tribhuwan Kumar ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture ◽  
In Vitro Production ◽  
Biotechnological Approach ◽  
Vitro Production

52 An in vitro model for determination of the rate of resistance development in HIV

1993 ◽  
Vol 20 ◽  
pp. 68 ◽  
Author(s):  
L. Vrang ◽  
C. Rydergȧrd ◽  
C. Ȧhgren ◽  
J. Wahlberg ◽  
M. Uhlén ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Resistance Development ◽  
Vitro Model

scholarly journals Comparative investigation of sesquiterpene components of essential oils originating from intact plants and hairy root chamomile cultures

2021 ◽  
Vol 6 (2) ◽  
pp. 028-049
Author(s):  
Éva Szőke ◽  
Éva Lemberkovics
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Hairy Root ◽  
Folk Medicine ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Comparative Investigation ◽  
Biologically Active

The importance of chamomile (Chamomilla recutita) inflorescence is widely known in classical and folk medicine, with the largest group of its effective constituents forming the essential oil (chamazulene, a-bisabolol, α-farnesene, trans-β-farnesene, spathulenol, cis/trans-en-in-dicycloethers). Among cultivated species, the Hungarian BK-2 contains more chamazulene in its essential oil than the German Degumil type, which is mainly cultivated for its a-bisabolol. Both components have important antiinflammatory activities. Wild populations can be easily distinguished from cultivated ones by their high amount of bisaboloides, particularly the flower of Hungarian Szabadkígyós wild type, which contained on average 48 % of the biologically active (-)-a-bisabolol. The population of Szabadkígyós has good salt tolerance which is important owing to global warming, because the proportion of saline areas is increasing worldwide. To keep the genome of Szabadkígyós having high (-)-a-bisabolol content, Szőke and research team used biotechnological methods. Sterile plantlets, were infected by Agrobacterium rhizogenes strains #A-4, #15834, #R-1601. The hairy root clones possessing the best growing and biosynthetical potential were multiplied for phytochemical investigations. Pharmacologically important compounds of their essential oils were followed in great detail. The amount of in vitro cultured terpenoids and polyin compounds was compared with that of in vivo plants. GC-MS studies showed that sterile chamomile cultures generated the most important terpenoid and polyin compounds characteristics of the mother plant. Berkheyaradulene, geranyl-isovalerat and cedrol as new components were identified in these sterile cultures. The main component of hairy root cultures (D/400, D/1, D/100 and Sz/400) was tr-b-farnesene and in addition one new compound: a-selinene was identified. Hairy root culture originated from chamomile collected in Szabadkígyós was intensive increased the essential oil content and pharmacological active compounds: (-) -α-bisabolol and β-eudesmol was also synthetized in large quantity. Furthermore, in vitro organized cultures were made from this population to obtain propagation material containing numerous active substances.

Determination of Functional ABCG2 Activity and Assessment of Drug–ABCG2 Interactions in Dairy Animals Using a Novel MDCKII In Vitro Model

2013 ◽  
Vol 102 (2) ◽  
pp. 772-784 ◽  
Author(s):  
Louise Wassermann ◽  
Sandra Halwachs ◽  
Stefan Lindner ◽  
Kerstin U. Honscha ◽  
Walther Honscha
Keyword(s):  
In Vitro Model ◽  
Dairy Animals ◽  
Vitro Model

scholarly journals Biotechnological Approaches for Bioremediation: In Vitro Hairy Root Culture

2017 ◽  
pp. 597-619 ◽  
Author(s):  
Sonia Malik ◽  
Sara Adrián L. Andrade ◽  
Mohammad Hossein Mirjalili ◽  
Randolph R. J. Arroo ◽  
Mercedes Bonfill ◽  
...  
Keyword(s):  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture

Clearance of Levofloxacin by an in Vitro Model of Continuous Venovenous Hemodialysis (CVVHD)

2007 ◽  
Vol 30 (10) ◽  
pp. 889-895 ◽  
Author(s):  
S. Siewert ◽  
B. Drewelow ◽  
S.C. Mueller
Keyword(s):  
In Vitro Model ◽  
Urea Clearance ◽  
Flow Rates ◽  
Dialysate Flow ◽  
Dialysate Flow Rate ◽  
Continuous Venovenous Hemodialysis ◽  
Vitro Model ◽  
Positive Correlations

Information about the elimination and the adequate dosing of levofloxacin during renal replacement therapy is scarce. The aim of this study was to characterize in vitro the elimination of levofloxacin during continuous venovenous hemodialysis (CVVHD) and to investigate whether the CVVHD clearances of creatinine and urea are correlated with the levofloxacin clearance in order to facilitate dosage adjustments. An in vitro model of CVVHD was established using five dialyzer membranes at varying dialysate flow rates applied in the clinical setting (8, 16, 25, 33 and 41 ml/min). Plasma and dialysate samples were drawn for determination of levofloxacin, creatinine and urea concentrations to evaluate clearances by CVVHD. During CVVHD, the clearance of levofloxacin varied between 9.02 and 33.30 ml/min, depending on the chosen setup. Positive correlations (p<0.001) were received for: dialysate flow rate (QD) and creatinine/urea clearances (R>0.93); QD and levofloxacin clearance (R 0.59–0.71); levofloxacin and creatinine clearance (R 0.69–0.75); and levofloxacin and urea clearance (R 0.56–0.75) as well. When dosing critically ill patients, therefore, extracorporeal as well as total clearance of levofloxacin should be considered.

Comparison of Two Different In Vivo Models and an In Vitro Model for Caloric Determination of Four Novel Fiber Ingredients

2013 ◽  
Vol 61 (50) ◽  
pp. 12374-12379 ◽  
Author(s):  
Sarah Cervantes-Pahm ◽  
Brenda K. Knapp ◽  
Beob G. Kim ◽  
Yanhong Liu ◽  
Carl M. Parsons ◽  
...  
Keyword(s):  
In Vitro Model ◽  
In Vivo Models ◽  
Vitro Model

Polyprenols in hairy roots of Coluria geoides

2000 ◽  
Vol 28 (6) ◽  
pp. 790-791 ◽  
Author(s):  
K. Skorupińska-Tudek ◽  
V. S. Hung ◽  
O. Olszowska ◽  
M. Furmanowa ◽  
T. Chojnacki ◽  
...  
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Lipid Content ◽  
Mammalian Cells ◽  
Root Culture ◽  
Dry Weight ◽  
Hairy Root Culture ◽  
Living Organisms ◽  
Long Chain

Long-chain polyisoprenoid alcohols built from several up to more than 100 isoprenoid units are common constituents of all living organisms. They were found mostly in plants, bacteria, yeasts and mammalian cells. In vitro hairy root culture of Coluria geoides was obtained from plants transformed with Agrobacterium rhizogenes. Growth was optimal at 0.75% (w/v) glucose and at 22 °C. Dry samples of roots were extracted and lipid content was analysed by HPLC. According to our estimation, polyprenols are accumulated in roots of C. geoides cultivated in vitro as a mixture of several prenologues with the dominating prenol composed of 16 isoprenoid units. The content of polyprenols in tissue was approx. 300 μg/g of dry weight.

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