scholarly journals Telomere lengths in women with early reproductive losses

2019 ◽  
Vol 24 ◽  
pp. 202-207
Author(s):  
N. I. Huleyuk ◽  
D. V. Zastavna ◽  
M. Tyrka
Keyword(s):  
Older Women ◽  
Reproductive Function ◽  
Peripheral Blood Lymphocytes ◽  
Control Group ◽  
Healthy Children ◽  
Rt Pcr ◽  
Reproductive Loss ◽  
Reproductive Losses ◽  
History Of ◽  
Polymerase Chain

Aim. Study of the relative telomere lengths in women with a history of early reproductive losses. Methods. Relative Telomere Length (RTL) was studied in the peripheral blood lymphocytes using a real time polymerase chain reaction (RT-PCR). Results. RTL was studied in 281 women, among which 169 - with early reproductive losses (ERL) (surveyed group) and 112 - have healthy children and no reproductive losses in history (control group). For women under the age of 35, the average RTL value is significantly higher than that of older women, P = 0.003597. In women with ERL, RTL is significantly lower than that of women with a preserved reproductive function, P = 0.0000001. The value of RTL is significantly lower in women with ERL under 35 years compared with control, P = 0.0000001, and is similar to value of RTL in women in the control group at the age of 36, P> 0.05. Conclusions. The telomere lengths is significantly lower in both older women and women with ERL. The similarity of RTLvalue in women with ERL up to 35 years of age and in women with a preserved reproductive function at the age of 36 years testifies to the telomeric theory of reproductive aging. Keywords: telomeres, RT-PCR, women, age, early reproductive loss.

scholarly journals Telomere length in individuals with early pregnancy losses

2021 ◽  
Vol 29 ◽  
pp. 147-151
Author(s):  
N. L. Huleyuk ◽  
D.V. Zastavna ◽  
I.Ye. Haiboniuk ◽  
I.R. Tkach ◽  
M. Tyrka
Keyword(s):  
Telomere Length ◽  
Early Pregnancy ◽  
Control Group ◽  
The Past ◽  
Relative Telomere Length ◽  
Reproductive Loss ◽  
History Of ◽  
Polymerase Chain ◽  
Telomere Biology ◽  
The Relationship

Aim. Over the past decade, telomere biology has become an important topic in the field of human reproduction.We focused on the relationship between relative telomere length (RTL) and tendency to early pregnancy loss (EPL) in humans. Methods. RTL was measured in DNA isolated from the blood samples using a real-time polymerase chain reaction approach. RTL was examined in control group (C) (N=209) – women (CW) (N=107) and men (CM) (N=102) who had healthy pregnancies with no history of infertility or miscarriage, and in group with EPL (N=445) – women (EPLW) (N=223) and men (EPLM) (N=212) who had single or more EPL. RTL data were analyzed by gender and reproductive history. Results. Women (CW+EPLW) have significantly higher RTL that men (CM+EPLM) (1.74±0.06 in women and 1.40±0.05 in men, P=0.000053). Average RTL were significantly lower in CM compared to CW (CW: 2.27±0.12 versus CM: 1.15±0.08, P=0.0000001), and were similar in EPLW and EPLM (1.50±0.06 in EPLW and 1.53±0.06 in EPLM, P=0.47). The EPLW group had significantly lower RTL than control (EPLW: 1.50±0.06 versus CW: 2.27±0.12, P=0.0000001). Average RTL were significantly lower in CM compared to EPLM (1.15±0.08 in CM and 1.53±0.06 in EPLM, P=0.00006). Conclusions. Women with no history of EPL have longer telomere than men. Woman with EPL have shorter telomere that women without miscarriage. In EPL group women and men have similar telomere length.Keywords: telomeres, RT-PCR, gender, early reproductive loss.

scholarly journals Seroprevalence of Antibodies against SARS-CoV-2 in Children with Juvenile Idiopathic Arthritis a Case-Control Study

2021 ◽  
Vol 10 (8) ◽  
pp. 1771
Author(s):  
Violetta Opoka-Winiarska ◽  
Ewelina Grywalska ◽  
Izabela Korona-Glowniak ◽  
Katarzyna Matuska ◽  
Anna Malm ◽  
...  
Keyword(s):  
Juvenile Idiopathic Arthritis ◽  
Disease Activity ◽  
Disease Activity Score ◽  
Juvenile Arthritis ◽  
Activity Score ◽  
Control Group ◽  
Healthy Children ◽  
Serum Samples ◽  
History Of ◽  
Novel Coronavirus

There is limited data on the effect of the novel coronavirus disease (COVID-19) caused by severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) on pediatric rheumatology. We examined the prevalence of antibodies against SARS-CoV-2 in children with juvenile idiopathic arthritis (JIA) and a negative history of COVID-19 and the correlation of the presence of these antibodies with disease activity measured by juvenile arthritis disease activity score (JADAS). In total, 62 patients diagnosed with JIA, under treatment with various antirheumatic drugs, and 32 healthy children (control group) were included. Serum samples were analyzed for inflammatory markers and antibodies and their state evaluated with the juvenile arthritis disease activity score (JADAS). JIA patients do not have a higher seroprevalence of anti-SARS-CoV-2 antibodies than healthy subjects. We found anti-SARS-CoV-2 antibodies in JIA patients who did not have a history of COVID-19. The study showed no unequivocal correlation between the presence of SARS-CoV-2 antibodies and JIA activity; therefore, this relationship requires further observation. We also identified a possible link between patients’ humoral immune response and disease-modifying antirheumatic treatment, which will be confirmed in follow-up studies.

scholarly journals Роль материнских полиморфных вариантов гена HLA-G3'UTR 14-bp *ins/del в реализации генетического детерминирования риска формирования спорадических септальных врожденных пороков сердца без хромосомных болезней

2019 ◽  
Author(s):  
A.V. Shabaldin ◽  
A.V. Tsepokina ◽  
A.V. Ponasenko ◽  
E.V. Shabaldina
Keyword(s):  
Heart Defects ◽  
Main Group ◽  
Control Group ◽  
Healthy Children ◽  
Rt Pcr ◽  
Homozygous Genotype ◽  
Xenobiotic Biotransformation ◽  
Pcr Method ◽  
Mathematical Processing ◽  
Chromosomal Diseases

Врожденные пороки сердца (ВПС) являются ведущей патологией среди всех пороков и аномалий развития плода. Известно, что тератогенный эффект ксенобиотика будет максимально представлен при нарушенных иммунных взаимодействиях в системе мать-эмбрион . Неоднократно показано, что женский гомозиготный генотип HLA-G 3UTR 14-bp ins/ins ассоциирован с репродуктивными потерями. Цель исследования. Изучить распределение аллелей и генотипов генов биотрансформации ксенобиотиков у женщин, имеющих детей с ВПС, носительниц вариантных генотипов HLA-G 3UTR 14-bp ins/del. Материал и методы. Обследованы 103 женщины, у которых дети при рождении имели врожденный порок сердца без хромосомных заболеваний и родословной историей, а также 103 женщины (контрольная группа), родивших двух здоровых детей и более. Типирование полиморфных сайтов генов HLA-G 3UTR 14-bpins/del, GSTM1 (rs74837985), CYP1A1 (rs1048943) CYP1A2 (rs35694136, rs762551) GSTT1 (rs2266633, rs2266637, rs2234953) GSTP1 (rs6591256, rs1695, rs1871042, rs1793068), GATA 6 (rs10454095) проводили методом RT-PCR. Результаты. Показано, что у женщин основной группы, являющихся носительницами гомозиготного генотипа 14-bp ins/ins HLA-G 3UTR, статистически значимо чаще встречались гетерозиготные генотипы GSTP1 (rs6591256) A/G (р0,01 отношение шансов ОШ5,1, 2,5710,27), GSTP (rs1793068) G/T (р0,01 ОШ5,1, 2,5710,27), CYP1A2 (rs762551) A/C (р0,01 ОШ6,81, 3,3513,73) и гомозиготный минорный генотип GSTP1 (rs1871042) T/T (р0,001 ОШ7,32, 3,6214,76) по сравнению с группой контроля. В основной группе женщин G/G rs74837985 GSTM1 статистически значимо реже (р0,01 ОШ 0,20, 0,06-0,59) встречался у носительниц 14-bp ins/ins HLA-G 3UTR по сравнению с носительницами гетерозиготного генотипа 14-bp ins/del HLA-G 3UTR. Заключение. В ходе исследования установлено, что при одновременном носительстве мутантных аллелей HLA-G и генов системы транформации ксенобиотиков риски рождения детей с дефектом межжелудочковой перегородки выше, чем при носительстве изолированных мутаций.Congenital heart defects (CHD) are the leading pathologies among all congenital malformations and fetal development abnormalities. It is known that the teratogenic effect of xenobiotic will be maximally presented in the case of disturbed immune interactions in the mother-embryo system. It has been repeatedly shown that the female homozygous genotype HLA-G 3UTR 14-bp ins/ins is associated with reproductive losses. The purpose of this study was to study the distribution of alleles and genotypes of xenobiotic biotransformation genes in women with children with CHD, carriers of variant HLA-G 3UTR 14-bp ins/del genotypes. Material and method: It was examined 103 women whose children had a ventricular septal defect (VSD) without chromosomal diseases and a pedigree history at birth, and 103 women (control group) who gave birth to two or more healthy children. The typing of polymorphic sites of the genes HLA-G 3UTR 14-bp ins/del, GSTM1 (rs74837985), CYP1A1 (rs1048943) CYP1A2 (rs35694136, rs762551) GSTT1 (rs2266633, rs2266637, rs2234953) GSTP1 (rs6591256, rs1695, rs1871042, rs1793068), GATA 6 (rs10454095) was performed by the RT-PCR method. Mathematical processing was carried out using the application software STATISTICA 8.0 (StatSoftInc., USA) and SNPstats. Results: It was shown that the heterozygous genotypes GSTP1 (rs6591256) A / G (p0.01, OR5.1 (2.5710.27)), GSTP (rs1793068) G / T (p0.01, OR5.1 (2.5710.27)), CYP1A2 (rs762551) A/C (p0.01, OR6.81 (3.3513.73)) and the homozygous minor genotype GSTP1 (rs1871042) T/T (p0.001, OR7.32 (3.6214.76) ) were statistically significantly more frequent in the main group of women who are carriers of the homozygous genotype 14-bp ins/ins HLA-G 3UTR, in comparison with the control group. In the main group of women G/G rs74837985 GSTM1 statistically significantly less (p0.01, OR0.20 (0.060.59)) was found in carriers of 14-bp ins / ins HLA-G 3UTR, in comparison with carriers of the heterozygous genotype 14-bp ins/del HLA-G 3UTR. Conclusion: It was found that with the simultaneous carrier of the mutant alleles of HLA-G and the genes of the xenobiotics transformation system, the risk of having children with VSD is higher, compared with the carriage of isolated mutations.

Detection of Human Parvovirus B19 Infection in Children with Chronic Haemolytic Anaemia in Benha University Hospitals

2021 ◽  
Vol 30 (2) ◽  
pp. 51-58
Author(s):  
Amal M. Matta ◽  
Elsayed M. Abd-Elghany ◽  
Abeer A. Aboelazm ◽  
Osama Abo. Zaki, ◽  
Doaa Abd. Shaker
Keyword(s):  
Hemolytic Anemia ◽  
Parvovirus B19 ◽  
Control Group ◽  
P Value ◽  
Healthy Children ◽  
Igg Antibodies ◽  
University Hospitals ◽  
History Of ◽  
Chronic Hemolytic Anemia ◽  
Apparently Healthy

Background: Due to the tropism of human parvovirus B19 to erythroid progenitor cells, infection in patients with an underlying hemolytic disorder such as thalassemia, hereditary spherocytosis, sickle cell disease and Glucose-6-phosphate dehydrogenase deficiency leads to suppression of erythrocyte formation, referred to as transient aplasia crisis (TAC), which may be life-threatening. Objectives: Detection of parvovirus B19 DNA and its IgG antibodies in the serum of children with chronic hemolytic anemia and in apparently healthy children in Benha University Hospitals. Methodology: The study was conducted on 80 children. Forty of them with chronic hemolytic anemia, they were subdivided into 2 groups, Group (1a) included 20 patients without history of aplastic crisis, Group (Ib) included 20 patients with a history of aplastic crisis and 40 age and sex-matched apparently healthy children representing control (Group II). All patients were subjected to full history taking, clinical examination and laboratory investigations. Parvovirus B19 IgG was measured using anti-parvovirus B19 ELISA kits (SUNRED), and parvovirus B19 DNA was detected by using nestedpolymerase chain reaction. Results: The seroprevalence of parvovirus B19 IgG was significantly higher (P value =0.016) in Group Ia (50%) (10 out of 20) and Group Ib (45%) (9 out of 20) than the control group (Group II) (17.5%) (7 out of 40). There was a significant positive correlation between anti-parvovirus B19 IgG and age of all patients, frequency of blood transfusion. The prevalence of parvovirus B19 DNA was 10% (2 out of 20) in group Ia and 30% (6 out of 20) in group Ib and no viral DNA was detected in the controls (P value=0.001). Although 42.3% (11 out of 26) of children with β thalassemia major had a detectable level of antiparvovirus B19 virus IgG antibodies, only (23.1%) (6 out of 26) of them had B19 DNA. Anti-parvovirus B19 IgG antibodies were detected in 4 children out of 5 children of sickle cell anemia (80%) but the the prevalence of Parvovirus B19 DNA was 20% among them. Conclusion: Measures to keep away from iatrogenic and nosocomial infection transmission should be implemented including screening of donated blood for parvovirus B19 especially blood given to patients with blood disorders. Recommendation: Data from this study support the need for introduction of an approved vaccine that mainly protects children with chronic hemolytic anemia against that infection.

scholarly journals The use of chest ultrasonography in suspected cases of COVID-19 in the emergency department

2021 ◽  
Vol 7 (1) ◽  
pp. FSO635
Author(s):  
Enrico Allegorico ◽  
Carlo Buonerba ◽  
Giorgio Bosso ◽  
Antonio Pagano ◽  
Giovanni Porta ◽  
...  
Keyword(s):  
Lung Ultrasound ◽  
Initial Assessment ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Variable Model ◽  
Chest Ultrasonography ◽  
History Of ◽  
Ultrasound Score ◽  
Polymerase Chain ◽  
Or History

Aim: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus-specific reverse transcriptase-polymerase chain reaction (RT-PCR) represents the diagnostic gold standard. We explored the value of chest ultrasonography to predict positivity to SARS-CoV-2 on RT-PCR in suspected COVID-19 cases. Patients & methods: Consecutive patients with suspect COVID-19 were included if they had fever and/or history of cough and/or dyspnea. Lung ultrasound score (LUSS) was computed according to published methods. Results: A total of 76 patients were included. A 3-variable model based on aspartate transaminase (AST) > upper limit of normal, LUSS >12 and body temperature >37.5°C yielded an overall accuracy of 91%. Conclusion: A simple LUSS-based model may represent a powerful tool for initial assessment in suspected cases of COVID-19.

scholarly journals Evaluation of The 10 Warning Signs In Immunodeficient Patients: Additional Suggestions

2021 ◽  
Author(s):  
Fadime Ceyda Eldeniz ◽  
Yahya Gül ◽  
Alaattin Yorulmaz ◽  
Şükrü Nail Güner ◽  
Sevgi Keles ◽  
...  
Keyword(s):  
Family History ◽  
Early Diagnosis ◽  
Chronic Diarrhea ◽  
Warning Signs ◽  
Control Group ◽  
Healthy Children ◽  
Physician Awareness ◽  
History Of ◽  
And Control ◽  
Immunological Evaluation

Abstract Objective: Ten warning signs of primary immunodeficiency (PID) were suggested by the Jeffrey Modell Foundation (JMF), to increase physician awareness of PID. These warning signs have not yet been evaluated for patients with secondary immunodeficiency (SID). This study investigated whether the 10 warning signs used for the diagnosis of PID are sufficient for the diagnosis of SID, and explored the possibility of additional signs.Methods: This prospective study was conducted between June and December 2020. The mothers of 162 patients with PID and SID, and mothers of 200 healthy children, were asked to complete a questionnaire about family and personal history in addition to the warning signs of PID developed by the JMF. A JMF score was created by giving one point for each “Yes” answer for the 10 warning signs of PID. Medical records of the patients were evaluated for possible additional warning signs for PID and SID. Results: The JMF scores of the PID (3.36 ± 1.65) and SID (3.72 ± 1.12) groups were significantly higher than the scores of the control group (0.34 ± 0.61) (p < 0.05). A sign for immunological evaluation in two patients without warning signs in the PID group was found to be chronic diarrhea. In addition to the 10 JMF warning signs, we found that consanguinity and a family history of tuberculosis were statistically significant in our PID group, compared with the SID and control groups. Conclusions: The JMF warning signs are important for early diagnosis of PID. Our study showed that these signs may also be used for the early diagnosis of SID in patients and, according to our results, in addition to the 10 JMF signs for PID, parental consanguinity, chronic diarrhea, and a family history of tuberculosis may also be considered warning signs for the early diagnosis of PID.

scholarly journals Efficacy of normal saline nasal spray and gargle on SARS-CoV-2 for prevention of COVID-19 pneumonia

2021 ◽  
Author(s):  
Uday Chatterjee ◽  
Ajay Chakraborty ◽  
Sishir Naskar ◽  
Bibhuti Saha ◽  
Bhaswati Bandyapadhyay ◽  
...  
Keyword(s):  
Nasal Spray ◽  
Normal Saline ◽  
Control Group ◽  
Study Group ◽  
Rt Pcr ◽  
Group 14 ◽  
Reaction Report ◽  
Polymerase Chain ◽  
Different Strains

Abstract Background: Role of microaspiration of mucus mixed with SARS-CoV-2 (severe acute respiratory syndrome corona virus 2) causing pneumonia is lacking in searched literature. Recently some authors have emphasized on microaspiration. SARS-CoV-2 primarily replicates in nasal mucosa and sheds in nasal mucus which travels down as microaspiration and causes pneumonia. We aimed to evaluate the efficacy of normal saline nasal spray and gargle (NSNSG) to wash off SARS-CoV-2 from nasal and pharyngeal mucosa to prevent microaspiration and pneumonia. Methods: From RT-PCR (reverse transcriptase polymerase chain reaction) report, we selected 62 patients for study group and 63 patients for control, having higher virion load i.e. cycle threshold (Ct) value 25 or less. Patients in study group were trained with NSNSG. We reviewed HRCT (high resolution computed tomogram) of lung in 56 patients of both groups for severity score (SS) in lung and was compared with initial HRCTResults: Thirty out of 62 (48%) of study group significantly (p=0·01) became RT-PCR negative following NSNSG compared to 16 out of 63 patients (25%) of control. Thirty one out of 34 patients (91%) of study group either improved or inhibited progression of SS in lung HRCT. In control group, 14 out of 22 patients (63%) also showed favourable findings. Nevertheless, study group significantly improved (p=0·028) in SS.Conclusions: NSNSG is significantly efficacious to wash off SARS-CoV-2 from nasal cavity and pharynx, and to break supply chain of SARS-CoV-2 from source to prevent microaspiration in lung alveoli and pneumonia. From that phenomenon we infer that SARS-CoV-2 as a ‘surface virus’ and it seems that vaccine resistant SARS-CoV-2 and different strains of SARS-CoV-2 would be washed off with NSNSG.

The role of interleukin-9 and interleukin-17 in myocarditis with different etiologies

2019 ◽  
Vol 44 (6) ◽  
pp. 797-802
Author(s):  
Tuğba Kandemir Gülmez ◽  
Can Acipayam ◽  
Metin Kilinç ◽  
Nurten Seringeç Akkeçeci
Keyword(s):  
Rheumatic Fever ◽  
Acute Rheumatic Fever ◽  
Troponin I ◽  
Control Group ◽  
Interleukin 17 ◽  
Healthy Children ◽  
Serum Samples ◽  
History Of ◽  
And Gender ◽  
Interleukin 9

Abstract Objective Myocarditis is an inflammatory disease of the cardiac muscle. Prognosis is most often good but, in some patients, the disease can be fulminant. Our aim with this study was to determine interleukin-9 (IL-9) and interleukin-17 (IL-17) levels in myocarditis cases with different etiologies. Materials and methods Thirty one patients with myocarditis and 30 healthy controls of similar age and gender without a history of chronic disease were included in the study. All 31 patients were clinically myocarditis. In some of these patients, the cause of myocarditis is acute rheumatic fever or Kawasaki disease. Serum samples of the patients were taken during diagnosis in order to analyze serum IL-9 and IL-17 levels and sedimentation rate, CRP, ASO, pro-BNP, CK-MB, and Troponin-I tests were performed. Results It was found that IL-17 levels were statistically significant in all acute rheumatic fever, Myocarditis and Kawasaki patients compared to the control group (p = 0.001) and that cut-off was 4.30 pg/mL. This value was determined to be 71% sensitive and 67% specific for IL-17 (AUC = 0.761). Conclusions Both of the mean and median levels of IL-17 were significantly higher in pediatric patients with myocarditis than in healthy children. Our study made us think that complications of myocarditis and associated morbidity can be prevented by IL17 inhibitors. The high levels of IL17 found in our study may be a reference for future study.

Involvement of Interferon‐γ + 874A/T Polymorphism in the Pathogenesis of and Therapeutic Response to Immune Thrombocytopenia

2019 ◽  
Vol 50 (3) ◽  
pp. e42-e49
Author(s):  
Hadi Rezaeeyan ◽  
Kaveh Jaseb ◽  
Gholam Abas Kaydani ◽  
Ali Amin Asnafi ◽  
Mohammad Hosein Masbi ◽  
...  
Keyword(s):  
Immune Thrombocytopenia ◽  
Disease Onset ◽  
Interferon Γ ◽  
Control Group ◽  
Before And After ◽  
Causes Of Disease ◽  
History Of ◽  
Allele Specific ◽  
Polymerase Chain ◽  
And Control

Abstract Background Immune thrombocytopenia (ITP) is an autoimmune disease characterized by symptoms of thrombocytopenia and bleeding due to production of autoantibodies against platelets. Recently, the occurrence of polymorphisms has been identified as one of the main causes of disease onset. Methods To conduct this study, we recruited 140 patients and control individuals with no history of platelet loss. After collection of specimens, the prevalence of interferon-γ polymorphism was evaluated using the allele-specific oligonucleotide–polymerase chain reaction (ASO-PCR) technique and confirmed by sequencing techniques. Results The results showed that the frequency of the AA genotype was higher in the control group, compared with patients with ITP; however, in the acute and chronic groups, the frequency of the AT genotype was higher than that of the AA genotype. We also discovered that there was no significant correlation between platelet counts before and after treatment, nor in its related parameters with interferon (IFN)–γ polymorphism. Conclusion rs2430561 does not seem to have any role in ITP pathogenesis and treatment response.

Sign in / Sign up

Export Citation Format

Share Document