scholarly journals Characteristics of mutant soybean lines at Satt100 and Satt319 loci linked with E7 gene

2018 ◽  
Vol 23 ◽  
pp. 52-56
Author(s):  
D. O. Zharikova ◽  
G. O. Chebotar ◽  
M. V. Vilgota ◽  
I. V. Temchenko ◽  
S. V. Chebotar
Keyword(s):  
Microsatellite Loci ◽  
Chemical Mutagenesis ◽  
E7 Gene ◽  
Pcr Products ◽  
Glycine Max L ◽  
Soybean Gene ◽  
M 12 ◽  
Length Of The Day ◽  
Mutant Lines ◽  
Dna Pcr

Aim. Аnalysis of genetic polymorphism of microsatellite loci Satt100 and Satt319 in 10 lines obtained by chemical mutagenesis, and 4 parental varieties – Oksana, Femida, Zolotysta, Podil’s’ka 416. Satt100 and Satt319 are flanking E7gene, which determines sensitivity of soybean to the length of the day in ripening phase. Methods. DNA were isolated from soybean seeds using the kit NeoPrep100 DNA. PCR were performed with microsatellites Satt100 and Satt319. The PCR products were fractionated in 7% polyacrylamide gels. Results. In the investigated lines were detected 5 alleles at the Satt100 locus and 3 alleles at the Satt319 locus. 42.9 % of the varieties and lines in this study were carriers of the «E» allele for Satt100. According to analysis of locus Satt319 64.2 % of varieties and lines were the carriers of «B» allele. Conclusions. Variety Oksana, mutant lines Oksana M №12, Femida M №29 – are carriers of the dominant allele E7. Variety Zolotysta and five mutant lines have recessive allele e7. In 75 % cases we have detected changes of alleles at microsatellite loci in mutant lines in comparison with parental forms. We assume that mutagenic reagents could affect at generative organs of soybean and lead totheir open flowering and crosspollinations. Keywords: Glycine max (L.), soybean, gene E7, microsatellite markers, photoperiodic sensitivity.

Allelism and molecular mapping of soybean necrotic root mutants

Genome ◽  
2008 ◽  
Vol 51 (4) ◽  
pp. 243-250 ◽  
Author(s):  
Reid G. Palmer ◽  
Lei Zhang ◽  
Zhiping Huang ◽  
Min Xu
Keyword(s):  
High Frequency ◽  
Molecular Mapping ◽  
Mapping Population ◽  
Low Frequency ◽  
Flower Color ◽  
Somatic Tissue ◽  
Chemical Mutagenesis ◽  
Glycine Max L ◽  
Mutant Lines ◽  
Simple Sequence

Mutability of the w4 flower color locus in soybean, Glycine max (L.) Merr., is conditioned by an allele designated w4-m. Germinal revertants recovered among self-pollinated progeny of mutable plants have been associated with the generation of necrotic root mutations, chlorophyll-deficiency mutations, and sterility mutations. A total of 24 necrotic root mutant lines were generated from a total of 24 independent reversion events at the w4-m locus. The initial mutable population included 4 mutable categories for w4-m, designated (1) low frequency of early excisions, (2) low frequency of late excisions, (3) high frequency of early excisions, and (4) high frequency of late excisions. These mutable categories were based upon flower phenotype, i.e., somatic tissue. A total of 22 of 24 necrotic root mutations occurred from germinal reversions classified in the high frequency of excision categories. Of these 22 mutants, 14 came from early excisions and 8 came from late excisions. These necrotic root mutants were allelic to 6 previously identified necrotic root mutants derived from the study of germinal revertants, i.e., gene tagging studies, chemical mutagenesis, and “spontaneous” occurrences from genetic crosses. Thus, all 30 necrotic root mutants in soybean are allelic. An F2 mapping population from the cross of Minsoy (Rn1 Rn1) × T328 (rn1 rn1) was used to map the Rn1 locus using simple sequence repeat (SSR) markers. The Rn1 locus was located between Satt288 and Satt612 on molecular linkage group G.

scholarly journals A Dual-Targeted Soybean Protein Is Involved in Bradyrhizobium japonicum Infection of Soybean Root Hair and Cortical Cells

2011 ◽  
Vol 24 (9) ◽  
pp. 1051-1060 ◽  
Author(s):  
Marc Libault ◽  
Manjula Govindarajulu ◽  
R. Howard Berg ◽  
Yee Tsuey Ong ◽  
Kari Puricelli ◽  
...  
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Soybean Protein ◽  
Carbon Utilization ◽  
Plant Host ◽  
Cortical Cells ◽  
Symbiotic Interaction ◽  
Carbon Supply ◽  
Glycine Max L ◽  
Soybean Gene

The symbiotic interaction between legumes and soil bacteria (e.g., soybean [Glycine max L.] and Bradyrhizobium japonicum]) leads to the development of a new root organ, the nodule, where bacteria differentiate into bacteroids that fix atmospheric nitrogen for assimilation by the plant host. In exchange, the host plant provides a steady carbon supply to the bacteroids. This carbon can be stored within the bacteroids in the form of poly-3-hydroxybutyrate granules. The formation of this symbiosis requires communication between both partners to regulate the balance between nitrogen fixation and carbon utilization. In the present study, we describe the soybean gene GmNMNa that is specifically expressed during the infection of soybean cells by B. japonicum. GmNMNa encodes a protein of unknown function. The GmNMNa protein was localized to the nucleolus and also to the mitochondria. Silencing of GmNMNa expression resulted in reduced nodulation, a reduction in the number of bacteroids per infected cell in the nodule, and a clear reduction in the accumulation of poly-3-hydroxybutyrate in the bacteroids. Our results highlight the role of the soybean GmNMNa gene in regulating symbiotic bacterial infection, potentially through the regulation of the accumulation of carbon reserves.

scholarly journals Nested PCR for the detection of Taenia solium DNA in stool samples

2020 ◽  
Author(s):  
C Franco-Muñoz ◽  
A Arévalo ◽  
S Duque-Beltran
Keyword(s):  
Detection Limit ◽  
Nested Pcr ◽  
Intestinal Parasites ◽  
Taenia Solium ◽  
Cross Reaction ◽  
Public Health Importance ◽  
Alkaline Lysis ◽  
Pcr Products ◽  
Stool Samples ◽  
Dna Pcr

ABSTRACTThe traditional parasitological method to diagnose taeniasis is the microscopic observation of eggs in stool samples. However, this method does not allow differentiation between Taenia saginata and Taenia solium. This aim of this study was to achieve the detection of T. solium DNA by polymerase chain reaction (PCR) and to evaluate the cross-reaction with other species of the genus Taenia and other intestinal parasites. DNA was extracted from adult T. solium cestodes by cryolysis in liquid nitrogen and with the DNA stool extraction kit from Qiagen. The detection limit of the test was evaluated by DNA dilutions in water and in stool samples. DNA was extracted from proglottids of T. saginata and T. crassiceps and from stool samples containing other intestinal parasites using ethanol treatment, alkaline lysis, and the DNA stool extraction kit. Nested PCR was used to amplify a previously described fragment of the Tso31 gene, and the PCR products were analyzed by electrophoresis in 2% agarose gels followed by staining with GelRed. The nested PCR of the Tso31 gene allowed the detection of T. solium DNA in stool samples with a detection limit of 20 pg of parasite DNA. PCR showed no cross-reaction with T. saginata, T. crassiceps, or other intestinal parasites of public health importance in Colombia.

scholarly journals Studies on Induced Physical and Chemical Mutagenesis in Groundnut (Arachis hypogia)

2015 ◽  
Vol 35 ◽  
pp. 25-35 ◽  
Author(s):  
A. Gunasekaran ◽  
P. Pavadai
Keyword(s):  
Gamma Rays ◽  
Seedling Survival ◽  
Dry Weight ◽  
Mutation Breeding ◽  
Chemical Mutagenesis ◽  
Program Selection ◽  
Methane Sulphonate ◽  
Ld50 Value ◽  
Mutant Lines ◽  
Physical And Chemical

Mutation breeding has been widely used for the improvement of plant characters in various crops. It is a powerful and effective tool in the hands of plant breeders. In any mutation breeding program, selection of an effective and efficient mutagen is very essential to produce high frequency of desirable mutation. Groundnut (Arachis hypogia) var. VRI-2. was treated with different concentration of physical and chemical mutagen namely gamma rays 10, 20, 30, 40, 50 and 60 KR and Ethyl methane sulphonate (EMS). For inducing mutation various concentration of EMS such as 0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 % for six hours were applied to 200 seed sample of each concentration and one respective control. The LD50 value was observed in 50% of gamma rays and 0.5 % of EMS. The morphological and yield characters were significantly reduced seed germination, seedling survival, days to first flower, plant height, number of leaves per plant, number of grains per plant, grain length and breath, 100 grains weight, grain yield per plant, fresh and dry weight per plant. The increasing doses/concentration of gamma rays and EMS decreased in phenotypic and yield characters in M1generation. The mutagenized populations showed significantly higher variability in the M2 generation. Mutant lines showing higher yield per plant than the respective parents and checks were isolated in M2 and subsequent generation were significantly more pod yield and yield components than the untreated plants.

scholarly journals Molecular characterization of chemical mutagenesis induced diversity in elite maize germplasm

Genetika ◽  
2004 ◽  
Vol 36 (1) ◽  
pp. 47-60 ◽  
Author(s):  
Nikolai Christov ◽  
Elena Todorovska ◽  
Dionysia Fasoula ◽  
Ioannis Ioannides ◽  
Atanas Atanassov ◽  
...  
Keyword(s):  
Flowering Time ◽  
Molecular Characterization ◽  
Pcr Amplification ◽  
Inbred Lines ◽  
Chromosome 1 ◽  
Chemical Mutagenesis ◽  
Maize Germplasm ◽  
Mutant Lines

Three classical breeding Iowa Super Stiff Stalk (SSS) inbred lines B37, B73 and B84, one Lancaster inbred Oh43 and mutant lines obtained by chemical mutagenesis followed by mutation breeding as follows: two of B37 and four of Oh43 were selected for molecular characterization. The mutant inbred lines were chosen because in addition to the improved GCA and SCA for grain yield, proven by their predominance in the Bulgarian breeding programs, they showed shifts in the flowering time as compared to the initial inbreds. Molecular markers (micro satellites and other PCR-based DNA markers) were used for characterization of maize genotypes and determination of the induced by chemical mutagenesis genetic variability in maize germplasm. The tested nine SSR markers (umc 1001, umclO14, umcl057, umcll81, umcl0lS, umc 1029. umcl003, umc 1033 and umcl035) can discriminate between the initial classical breeding inbred lines and the originating mutant inbreds. Allelic diversity was also studied by PCR amplification with specifically de-signed primers in the coding regions and flanking sequence of two genes: dwarf8 (d&: chromosome 1, 198.5 cM), and indeterminate l (id1; chromosome 1. 175.0 cM). These are considered candidate genes for variation in plant height and/or flowering time, based on mutant phenotypes and chromosomal locations near major QTLs. Single nucleotide polymorphisms and indels were detected in the region flanking the SH2 domain of dwarf8 gene in some of the mutant inbreds as a result of SSCP and sequencing analyses. However, these polymorphisms could not be associated with the observed variations in flowering time. PCR analysis of the promoter region dwarf8 showed a variant fragment of about 1 kb in the inbred line Oh43 that was not present in any other initial and mutant in-bred lines included in the study. PCR amplification of the 5' end of the Id1 coding sequence revealed polymorphic bands in the mutant lines XM535, XM521, XM250-l, XM98-8 and XM85-105, as well as in the classical breeding line B73. The data, presented here demonstrate the usefulness of chemical mutagenesis for generation of genetic diversity within the elite maize germplasm. Some of this variation may affect the major genes in the QTLs. Our initial data revealed mutagenesis induced polymorphisms in the coding sequences of two important for the determination of flowering time transcription factors. Further molecular analyses of the proposed model systems may complement the trait association efforts and will help to directly identify the major genes in the QTLs.

scholarly journals Phenotypic and molecular cytogenetic variability in calendula (Calendula officinalis L.) cultivars and mutant lines obtained via chemical mutagenesis

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Tatiana E. Samatadze ◽  
Svyatoslav A. Zoshchuk ◽  
Firdaus M. Hazieva ◽  
Olga Yu Yurkevich ◽  
Natalya Yu Svistunova ◽  
...  
Keyword(s):  
Chemical Mutagenesis ◽  
Calendula Officinalis ◽  
Molecular Cytogenetic ◽  
Mutant Lines ◽  
Calendula Officinalis L

scholarly journals Molecular typing ofCryptosporidium parvumassociated with a diarrhoea outbreak identifies two sources of exposure

2007 ◽  
Vol 136 (8) ◽  
pp. 1147-1152 ◽  
Author(s):  
J. G. MATTSSON ◽  
M. INSULANDER ◽  
M. LEBBAD ◽  
C. BJÖRKMAN ◽  
B. SVENUNGSSON
Keyword(s):  
Cryptosporidium Parvum ◽  
Microsatellite Loci ◽  
Molecular Typing ◽  
Swimming Pool ◽  
Molecular Fingerprinting ◽  
Epidemiological Investigation ◽  
Pool Water ◽  
Pcr Products ◽  
Swimming Pool Water

SUMMARYAn outbreak of cryptosporidiosis associated with exposure to outdoor swimming-pool water affected an estimated 800–1000 individuals. PCR products were obtained from faecal specimens from 30 individuals who tested positive forCryptosporidiumoocysts. RFLP and sequencing analyses showed that all individuals were infected withCryptosporidium parvum. Among the infected individuals, five had just swum in an adjacent indoor pool during the same period, and had no identified contact with individuals linked to the outdoor pool. With the use of subgenotyping based on analysis of three mini- and microsatellite loci, MS1, TP14, and GP15, we could identify two sources of exposure. One subtype was associated with the outdoor pool and another with the indoor pool. These data demonstrate that the use of mini- and microsatellite loci as markers for molecular fingerprinting ofC. parvumisolates are valuable in the epidemiological investigation of outbreaks.

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