scholarly journals DNA certification of varieties and hybrids of perennial grass crops

2018 ◽  
Vol 22 ◽  
pp. 267-273
Author(s):  
I. P. Kondratskaya ◽  
A. N. Yukhimuk ◽  
O. V. Chizhik ◽  
V. N. Reshetnikov ◽  
V. A. Stolepchenko ◽  
...  
Keyword(s):  
Lolium Perenne ◽  
Molecular Genetic ◽  
Perennial Grass ◽  
Perennial Grasses ◽  
Agropyron Cristatum ◽  
Dna Labeling ◽  
First Time ◽  
Dna Pcr ◽  
Issr Primers ◽  
Genetic Passport

Aim. The aim of this study was to carry out passportization of cultivars, varieties and hybrids of perennial grass crops: Alopecurus protensis, Agropyron cristatum, Lolium perenne, Lolium miltiflorum, Festuca protensis, Festuca arundinasea, hybrids Festulolium. Methods. The perennial grasses selection was carried out by embryo culture according to methodical recommendations. For the molecular-genetic passportization of perennial grasses varieties the polymorphous primers have been selected – 9 RAPD primers and 15 ISSR primers. Results. Multilocus DNA labeling have allowed to differentiate all the genotypes being studied, to develop and to create the unique profiles for each of them and to calculate the genetic distance of congeniality/distances. On the base of RAPD/ISSR, SCoT spectra the 14 molecular-genetic passports of perennial grass cops have been obtained for the first time. Keywords: grass crops, molecular-genetic passport, DNA, PCR, multipoint mapping RAPD/ISSR spectra.

Molecular genetic linkage maps for allotetraploid Leymus wildryes (Gramineae: Triticeae)

Genome ◽  
2003 ◽  
Vol 46 (4) ◽  
pp. 627-646 ◽  
Author(s):  
Xiaolei Wu ◽  
Steven R Larson ◽  
Zanmin Hu ◽  
Antonio J Palazzo ◽  
Thomas A Jones ◽  
...  
Keyword(s):  
Molecular Genetic ◽  
Perennial Grass ◽  
Perennial Grasses ◽  
Genetic Maps ◽  
Aflp Markers ◽  
Linkage Maps ◽  
Linkage Groups ◽  
Genetic Linkage Maps ◽  
Leymus Triticoides ◽  
Leymus Cinereus

Molecular genetic maps were constructed for two full-sib populations, TTC1 and TTC2, derived from two Leymus triticoides × Leymus cinereus hybrids and one common Leymus triticoides tester. Informative DNA markers were detected using 21 EcoRI–MseI and 17 PstI–MseI AFLP primer combinations, 36 anchored SSR or STS primer pairs, and 9 anchored RFLP probes. The 164-sib TTC1 map includes 1069 AFLP markers and 38 anchor loci in 14 linkage groups spanning 2001 cM. The 170-sib TTC2 map contains 1002 AFLP markers and 36 anchor loci in 14 linkage groups spanning 2066 cM. Some 488 homologous AFLP loci and 24 anchor markers detected in both populations showed similar map order. Thus, 1583 AFLP markers and 50 anchor loci were mapped into 14 linkage groups, which evidently correspond to the 14 chromosomes of allotetraploid Leymus (2n = 4x = 28). Synteny of two or more anchor markers from each of the seven homoeologous wheat and barley chromosomes was detected for 12 of the 14 Leymus linkage groups. Moreover, two distinct sets of genome-specific STS markers were identified in these allotetraploid Leymus species. These Leymus genetic maps and populations will provide a useful system to evaluate the inheritance of functionally important traits of two divergent perennial grass species.Key words: AFLP, perennial grasses, RFLP, STS, SSR.

Competition among seedlings of perennial grasses, subterranean clover, white clover, and annual ryegrass in replacement series mixtures

2000 ◽  
Vol 51 (3) ◽  
pp. 377 ◽  
Author(s):  
G. M. Lodge
Keyword(s):  
White Clover ◽  
Plant Competition ◽  
Perennial Grass ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Perennial Grasses ◽  
Maximum Likelihood Estimates ◽  
Annual Ryegrass ◽  
Replacement Series ◽  
Successful Establishment

Seedlings of 3 perennial grasses, Danthonia linkii Kunthcv. Bunderra, D. richardsonii Cashmore cv. Taranna(wallaby grasses), and Phalaris aquatica L. cv. Sirosa,were each grown in replacement series mixtures with seedlings ofTrifolium repens L. (white clover),Trifolium subterraneum L. var. brachycalycinum (Katzn.et Morley) Zorahy & Heller cv. Clare (subterraneanclover), and Lolium rigidum L. (annual ryegrass). Plantswere sown 5 cm apart in boxes (45 by 29 by 20 cm) at a density of 307plants/m2. Maximum likelihood estimates were usedto derive parameters of a non-linear competition model using the dry matterweights of perennial grasses and competitors at 3 harvests, approximately 168,216, and 271 days after sowing. Intra-plant competition was examined inmonocultures of each species, grown at plant spacings of 2, 5, and 8 cm apartwith plants harvested at the above times.Competition occurred in all perennial grass–competitor mixtures, exceptin those of each perennial grass with white clover and thephalaris–subterranean clover mixture (Harvest 1) and those withD. richardsonii and phalaris grown with white clover(Harvest 2). For D. richardsonii (Harvests 1 and 2) andD. linkii (Harvest 1 only) grown with white clover andthe phalaris–subterranean clover (Harvest 1), the two species in themixture were not competing. In the phalaris–white clover mixture, eachspecies was equally competitive (Harvests 1 and 2). These differences incompetition and aggressiveness reflected differences in individual plantweights in monocultures where there was an effect (P < 0.05) of species ondry matter weight per box, but no significant effect of plant spacing.These data indicated that for successful establishment,D. richardsonii and D. linkiishould not be sown in swards with either subterranean clover or white clover,or where populations of annual ryegrass seedlings are likely to be high.Phalaris was more compatible with both white clover and subterranean clover,but aggressively competed with by annual ryegrass.

Satellite derived maps of pasture growth status: association of classification with botanical composition

1997 ◽  
Vol 37 (5) ◽  
pp. 547 ◽  
Author(s):  
P. J. Vickery ◽  
M. J. Hill ◽  
G. E. Donald
Keyword(s):  
Near Infrared ◽  
Perennial Grass ◽  
Vegetation Indices ◽  
Fast Growth ◽  
Perennial Grasses ◽  
Bayesian Probability ◽  
Cumulative Probability ◽  
Botanical Composition ◽  
South Wales ◽  
Native Pastures

Summary. Spectral data from the green, red and near-infrared bands of Landsat MSS and Landsat TM satellite imagery acquired in mid-spring were classified into 3 and 6 pasture growth classes respectively. The classifications were compared with a site database of botanical composition for the Northern Tablelands of New South Wales to examine the association between spectral growth class and pasture composition. Pastures ranged in composition from unimproved native perennial grasses through semi-improved mixtures of native and naturalised grasses and legumes to highly improved temperate perennial grasses and legumes. For 3 years of MSS data, the fast growth class had a mean botanical composition of about 80% improved perennial grass and 0% native; medium growth class averaged 46% improved perennial grass and 14% native; while the slow growth class had about 60% native and 1% improved perennial grass when averaged over 3 years of MSS data. For the 6 class TM data from a single year, a predictive logistic regression of cumulative probability was developed for percentage of ‘very fast’ growth pixels and ordered 10 percentile categories of improved perennial grass or native grass. Differences in patch characteristics between classes with MSS disappeared with TM reclassified to the same 3 class level. Most probable pasture type was inferred from 3 class MSS and TM data using Bayesian probability analysis. The resulting maps were similar in general appearance but detail was better with the TM data. The pasture growth classification identified highly improved perennial grass pastures and native pastures but sensitivity to intermediate pasture types was poor. Future improvement will come from direct measurement of biophysical characteristics using vegetation indices or inversion of reflectance models.

Gymnocolea borealis (Anastrophyllaceae, Marchantiophyta) in Asia and Russia: morphology, ecology, distribution, and differentiation

2021 ◽  
Vol 55 (2) ◽  
pp. 487-494
Author(s):  
A. D. Potemkin ◽  
A. A. Vilnet ◽  
E. I. Troeva ◽  
K. A. Ermokhina
Keyword(s):  
Molecular Genetic ◽  
Morphological Description ◽  
Mire Vegetation ◽  
Circumpolar Distribution ◽  
Siberian Arctic ◽  
Cpdna Sequence ◽  
First Time ◽  
Genetic Comparison

Gymnocolea borealis is reported for the first time for Asia from Russia based on the morphological and subsequent molecular-genetic comparison of rbcL cpDNA sequence of the specimen from the Gydansky Peninsula, West Siberian Arctic. An extended morphological description, generalizing the species characters throughout its range, and data on its variation, differentiation and ecology, and photomicrographs are provided. The occurrence of G. borealis growing as separate shoots among dense mire vegetation makes it easy to overlook. Presently known isolated records of G. borealis support its disjunctive circumpolar distribution.

Molecular genetic diagnostics of Bardet-Biedl syndrome with an MPS-panel

2021 ◽  
pp. 26-35
Author(s):  
М.Д. Орлова ◽  
П. Гундорова ◽  
А.В. Поляков
Keyword(s):  
Autosomal Recessive ◽  
Molecular Genetic ◽  
Autosomal Recessive Disorder ◽  
Genetic Diagnostics ◽  
Bardet Biedl Syndrome ◽  
Pathogenic Variants ◽  
Development Delay ◽  
Molecular Genetic Diagnostics ◽  
First Time

Синдром Барде-Бидля - аутосомно-рецессивное заболевание, характеризующееся ожирением, пигментной дегенерацией сетчатки, полидактилией, задержкой психоречевого развития и структурными повреждениями почек. В работе представлены результаты применения МПС-панели, включающей кодирующие последовательности и прилегающие интронные области 21 гена, ассоциированного с синдромом Барде-Бидля. Впервые была проведена молекулярно-генетическая диагностика в группе из сорока российских пациентов с синдромом Барде-Бидля из неродственных семей. В результате исследования удалось подтвердить диагноз молекулярно-генетическим методом у 40% пациентов (n=16). В генах BBS1, BBS7 и BBS10 встретились повторяющиеся варианты. Частота встречаемости патогенных и вероятно патогенных вариантов в генах BBS1 и BBS10 у российских пациентов соответствует зарубежным данным. Варианты в гене BBS7 встретились у пяти человек, у четырех из них был обнаружен патогенный вариант c.1967_1968delTAinsC, не встречающийся в других популяциях. Результаты, представленные в статье, показывают значительный вклад в заболеваемость синдромом Барде-Бидля в российской популяции патогенных вариантов в гене BBS7. Bardet-Biedl syndrome is an autosomal recessive disorder characterized by obesity, retinitis pigmentosa, polydactyly, development delay, and structural kidney defects. This study shows the results of using an MPS panel that includes coding sequences and intronic areas of 21 genes associated with Bardet-Biedl syndrome. For the first time molecular genetic testing has been provided for the group of 40 Russian patiens with Bardet-Biedl syndrome from unrelated families. As a result of the testing, diagnoses were confirmed for 40% of the patients (n=16). The genes BBS1, BBS7, BBS10 had recurrent variants. The frequency of pathogenic and likely pathogenic variants in the genes BBS1 and BBS10 among Russian patients matches the research data in other countries. Variants in the BBS7 gene were found for five people, four of them had a pathogenic variant c.1967_1968delTAinsC, which is not present among other populations. Results provided in this article show the significant role of pathogenic variants in the BBS7 gene in patients with Bardet-Biedl syndrome in Russian population.

Sources of valuable traits for perennial grass breeding

2021 ◽  
pp. 78-89
Author(s):  
LZ Baistruk-Hlodan ◽  
MM Кhomiak ◽  
HZ Zhapaleu
Keyword(s):  
Festuca Arundinacea ◽  
Trifolium Pratense ◽  
Perennial Grass ◽  
High Energy ◽  
Phleum Pratense ◽  
Perennial Grasses ◽  
Starting Material ◽  
Forage Production ◽  
Daily Growth ◽  
Galega Orientalis

Aim. The purpose was to identify collection accessions – sources of valuable traits to use as starting material for creating varieties of perennial grasses in Western Ukraine. Results and Discussion. Perennial grasses play an essential role in improving the efficiency of forage production. They produce a fodder mass that contains major macro- and micronutrients, minerals, vitamins, amino acids, and other nutrients in available forms, with a high energy protein saturation. Practice shows that due to the introduction of varietal crops into production in combination with optimal technologies of their cultivation, which allows revealing the potential of each variety, it is possible to additionally obtain 20-30% higher yields of fodder mass annually and harvest 2 to 3-fold seed yields. In 2016-2020, a search was carried out and 570 new accessions of perennial grasses were recruited, of which 201 were legumes and 369 were graminaceous grasses. The collection contains 1,319 accessions, of which 232 are Trifolium pratense L., 115 are Trifolium repens L., 49 are Trifolium hybridum L., 107 are Lotus corniculatus L., 80 belong to other legume species (Medicago, Galega orientalis L. Galega orientalis L. and Trifolium species), 131 are Phleum pratense L., 187 are Dactylis glomerata L., 146 are Lolium perenne L., 53 are Arrhenatherum elatius (L.) J. et C.Presl., 67 are Festuca rubra L., 28 are Festuca trachyphylla L., 32 are Bromopsis inermis (Leyss.) Holub, 92 belong to other species of other types of graminaceous grasses (Festuca pratensis Huds., Festuca arundinacea Schreb., Agrostis alba L. etc.). Accessions that enter the Department are registered and sown for propagation in the field. After examination, valuable accessions are transferred to the National Depository and registered in the National Catalogue; the rest of the obtained seeds are used in working collections. Conclusions. The best collection accessions were identified by a set of economically valuable traits: sources of daily growth of shoots (30), winter hardiness (28), plant height (22), yield of green mass upon haymaking (28) and pasture (19) use, forage productivity (15), seed productivity (25), foliage (21), and disease resistance (23). They can be recommended as starting material to create varieties of perennial grasses with high yields of forage mass and seeds for various applications.

scholarly journals The search for alternative temperate perennial pasture species for low to medium rainfall environments in Tasmania

2016 ◽  
Vol 16 ◽  
pp. 275-279
Author(s):  
E.J. Hall ◽  
R. Reid ◽  
B. Clark ◽  
R. Dent
Keyword(s):  
Perennial Grass ◽  
Dactylis Glomerata ◽  
Red Clover ◽  
Perennial Grasses ◽  
Grass Species ◽  
Sheep Grazing ◽  
Cool Temperate ◽  
Perennial Legume ◽  
Perennial Legumes ◽  
Pasture Plants

In response to the need to find better adapted and more persistent perennial pasture plants for the dryland pastures in the cool-temperate low to medium rainfall (500-700 mm) regions, over 1000 accessions representing 24 species of perennial legumes and 64 species of perennial grasses, were introduced, characterised and evaluated for production and persistence under sheep grazing at sites throughout Tasmania. The work has identified four alternative legume species in Talish Clover (Trifolium tumens). Caucasian Clover (T. ambiguum), Stoloniferous Red Clover (T. pratense var. stoloniferum), Lucerne x Yellow Lucerne Hybrid (Medicago sativa x M.sativa subsp. falcata); and two grass species in Coloured Brome (Bromus coloratus) and Hispanic Cocksfoot (Dactylis glomerata var hispanica). Keywords: persistence, perennial grass, perennial legume

scholarly journals Improving soil properties and productivity of crop-pasture rotation in arid steppe of the Southern Urals

2021 ◽  
Vol 36 ◽  
pp. 03003
Author(s):  
Yakhiya Kaipov ◽  
Rifkat Akchurin ◽  
Rustam Kirayev ◽  
Asiya Nizaeva
Keyword(s):  
Soil Properties ◽  
Crop Rotation ◽  
Field Experiments ◽  
Humus Content ◽  
Perennial Grass ◽  
Southern Urals ◽  
Perennial Grasses ◽  
Reduced Tillage ◽  
The Southern Urals ◽  
Arid Steppe

Field experiments were conducted in the arid steppe zone of the Southern Urals, in the Republic of Bashkortostan of the Russian Federation. The soil is common chernozem. The precipitation during the growing season (May-September) is 166 mm. The experimental crop rotation consisted of 7 fields: 4 with perennial herbs (a mixture of Bromus inermis and Medicago varia), 3 with annual crops. The study analysed soil properties and carrying capacity in perennial grass rotation, conventional and reduced tillage. During the crop-pasture rotation, the humus content in the soil changes insignificantly depending on the tillage, being within 7.6-8.0 %. Perennial grasses loosened the soil, positively affecting moisture accumulation under crop rotation by the beginning of the pre-sowing period. The arable layer of common chernozem in the reduced tillage had a density of 1.05 g/cm3, less than 0.06 g/cm3 in conventional cultivation. Fertilizer application increased yields at 0.49-0.51 t/ha of feed units. Reduced tillage resulted in higher feed units by 0.06-0.08 tons per 1 ha. Crop-pasture rotation implementation and development ensures bioclimatically-determined yields of fodder crops and maintains soil fertility at an optimal level.

scholarly journals Study Sowing Grasslands Based on Red Clover with Perennial Grasses

2011 ◽  
Vol 68 (1) ◽  
Author(s):  
Denes DEAK ◽  
Ioan ROTAR ◽  
Florin PACURAR ◽  
Anca BOGDAN
Keyword(s):  
Trifolium Pratense ◽  
Poa Pratensis ◽  
Lolium Multiflorum ◽  
Perennial Grass ◽  
Red Clover ◽  
Phleum Pratense ◽  
Perennial Grasses ◽  
Ecological Niches ◽  
High Productivity ◽  
Stable Manure

Seeded lawns is one of the most important links in the process of improving the forage base, ensure feed quality with high productivity. Mixtures of red clover crops (Trifolium pratense) with perennial grasses (Lolium multiflorum, Phleum pratense and Poa pratensis) has high productivity due to better utilization of ecological niches of the biotope (ROTAR I.et al.). These crops has advantages like high content of protein because of the red clover, economy-based fertilizer nitrogen from atmospheric nitrogen fixation by bacteria Rhizobium spp. located in the root of legumes. These seeded pastures get a balanced feed nutrients (proteins, carbohydrates, lipids), have a high palatability. The species Trifolium pratense has a greater capacity to restore the soil structure and also the enrichment of the macro-elements, like phosphorus and potassium (CARLIER L., et. al). Our experience took place in the village Simonesti, Cobătesti village of the Harghita county. The experimental field was located respecting the experimental technique rules in randomized blocks with a technique that includes three variants based on red clover plus a perennial grass (Lolium multiflorum, Poa pratensis and Phleum pratense). Every version was fertilized with two types of fertilizer: one liquid (gull) and one solid (stable manure) in four different doses in all three variants. The doses were: V1 = 0 gull; V2 = 5 t / ha gull; V3 = 10 t / ha gull; V4 = 20 t / ha gulle and V1 = 0 stable manure; V2 = 10 t / ha stable manure; V3 = 30 t / ha stable manure and V4 = 50 t / ha stable manure. In our studies we present the influence of fertilization with gull and stable manure on yield of green mass of all three variants. In general, both gull fertilization with manure favors grasses at the expense of installing legumes. The higher doses of fertilizer increase, the share of participant of grasses increases.

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