scholarly journals Combined effect of genes ETR1 and ETR2 roots for branching Arabidopsis thaliana (L.) Heynh.

1970 ◽  
Vol 21 ◽  
pp. 216-220
Author(s):  
S. G. Khablak
Keyword(s):  
Root System ◽  
First Generation ◽  
Lateral Roots ◽  
Comparative Morphology ◽  
Gene Interactions ◽  
Arabidopsis Root ◽  
Arabidopsis Mutant ◽  
F2 Generation ◽  
Mutant Lines ◽  
Initial Forms

Aim. The aim of research is the study of inheritance the root system of A. thaliana in the interaction the genes ETR1 and ETR2. Methods. Comparative morphology – to compare the similarities and differences in the structure of the root systems plants, hybridlogical analysis crossing mutant lines and genetic analysis of inheritance of characteristics of the root system. Results. It was found that by crossing plants of the mutant lines Etr1-1 x Etr2-1 in the F2 generation occurs polymeric gene interactions ETR1 and ETR2. The splitting in this case is F2 9: 6: 1. In the first generation hybrids somatic heterosis is observed, which is manifested in a more powerful development of lateral roots compared to the initial forms. In the second generation is the process of splitting of hybrids, and their superiority over the length of lateral roots of parental forms is reduced. Conclusions. The results of the analysis carried out by a cross between plants of Arabidopsis mutant lines (Etr1-1 x Etr2-1) showed that the inheritance of characteristics of the root system in the interaction ETR1 and ETR2 genes occurs on the type of polymer gene action. Keywords: arabidopsis, root system, gene, mutation, heterosis.

scholarly journals The role of allelic and nonallelic interactions of genes in the mechanism of heterosis

2019 ◽  
Vol 24 ◽  
pp. 177-182
Author(s):  
S. G. Hablak ◽  
Ya. A. Abdullaeva ◽  
L. O. Ryabovol ◽  
Ya. S. Ryabovol
Keyword(s):  
First Generation ◽  
Gene Interaction ◽  
Point Of View ◽  
F1 Hybrids ◽  
Heterosis Effect ◽  
Nonallelic Interactions ◽  
F2 Generation ◽  
Mutant Lines ◽  
Polymeric Interaction

Aim. Studying the heterosis effect from the point of view of allelic and nonallelic gene interaction in F1 hybrids from crossing the arabidopsis ecotypes Col-0 and La-0. Methods. Hybridological method of crossing mutant lines and analysis of the inheritance of the signs of the root system. Results. When plants of different races Col-0 and La-0 cross in the F2 generation, polymeric interaction of genes occurs. In that case splitting in F2 goes in the ratio 15: 1. At the same time, hybrids of the first generation exhibit heterosis, which is manifested in a more powerful development of a rosette of leaves in comparison with the initial forms. Conclusions. The emergence of heterosis in hybrids of the first generation can be explained on the basis of the allelic and nonallelic gene interaction, which creates a favorable combination of genes during hybridization, causing the best manifestation of an economically valuable trait. Keywords: Arabidopsis thaliana (L.) Heynh., heterosis, gene, mutation, race.

scholarly journals Peroxisomal PEX7 Receptor Affects Cadmium-Induced ROS and Auxin Homeostasis in Arabidopsis Root System

Antioxidants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1494
Author(s):  
Diego Piacentini ◽  
Federica Della Rovere ◽  
Ilaria Bertoldi ◽  
Lorenzo Massimi ◽  
Adriano Sofo ◽  
...  
Keyword(s):  
Root System ◽  
Stress Responses ◽  
Lateral Roots ◽  
Reactive Oxygen ◽  
Nitrogen Species ◽  
Ros Scavenging ◽  
Cd Uptake ◽  
Arabidopsis Root ◽  
Antioxidant Defense Enzymes

Peroxisomes are important in plant physiological functions and stress responses. Through the production of reactive oxygen and nitrogen species (ROS and RNS), and antioxidant defense enzymes, peroxisomes control cellular redox homeostasis. Peroxin (PEX) proteins, such as PEX7 and PEX5, recognize peroxisome targeting signals (PTS1/PTS2) important for transporting proteins from cytosol to peroxisomal matrix. pex7-1 mutant displays reduced PTS2 protein import and altered peroxisomal metabolism. In this research we analyzed the role of PEX7 in the Arabidopsis thaliana root system exposed to 30 or 60 μM CdSO4. Cd uptake and translocation, indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) levels, and reactive oxygen species (ROS) and reactive nitrogen species (RNS) levels and catalase activity were analyzed in pex7-1 mutant primary and lateral roots in comparison with the wild type (wt). The peroxisomal defect due to PEX7 mutation did not reduce Cd-uptake but reduced its translocation to the shoot and the root cell peroxisomal signal detected by 8-(4-Nitrophenyl) Bodipy (N-BODIPY) probe. The trend of nitric oxide (NO) and peroxynitrite in pex7-1 roots, exposed/not exposed to Cd, was as in wt, with the higher Cd-concentration inducing higher levels of these RNS. By contrast, PEX7 mutation caused changes in Cd-induced hydrogen peroxide (H2O2) and superoxide anion (O2●−) levels in the roots, delaying ROS-scavenging. Results show that PEX7 is involved in counteracting Cd toxicity in Arabidopsis root system by controlling ROS metabolism and affecting auxin levels. These results add further information to the important role of peroxisomes in plant responses to Cd.

Utilization of a hydrate cellulose film for investigation of Arabidopsis thaliana L. root system growth and development

2020 ◽  
Vol 36 (1) ◽  
pp. 36-43
Author(s):  
I.O. Konovalova ◽  
T.N. Kudelina ◽  
S.O. Smolyanina ◽  
A.I. Lilienberg ◽  
T.N. Bibikova
Keyword(s):  
Arabidopsis Thaliana ◽  
Root System ◽  
Life Support ◽  
Higher Plants ◽  
Plant Root ◽  
Lateral Roots ◽  
Basic Research ◽  
Cellulose Film ◽  
A New Technique ◽  
Basic Research Project

A new technique for Arabidopsis thaliana cultivation has been proposed that combines the use of a phytogel-based nutrient medium and a hydrophilic membrane of hydrate cellulose film, separating the root system of the plant from the medium thickness. Growth rates of both main and lateral roots were faster in the plants cultivated on the surface of hydrate cellulose film than in the plants grown in the phytogel volume. The location of the root system on the surface of the transparent hydrate film simplifies its observation and analysis and facilitates plant transplantation with preservation of the root system configuration. The proposed technique allowed us to first assess the effect of exogenous auxin on the growth of lateral roots at the 5-6 developmental stage. methods to study plant root systems, hydrate cellulose film, A. thaliana, lateral roots, differential root growth rate, auxin The work was financially supported by the Russian Foundation for Basic Research (Project Bel_mol_a 19-54-04015) and the basic topic of the Russian Academy of Sciences - IBMP RAS «Regularities of the Influence of Extreme Environmental Factors on the Processes of Cultivation of Higher Plants and the Development of Japanese Quail Tissues at Different Stages of its Ontogenesis under the Conditions of Regenerative Life Support Systems».

Lateral root formation and nutrients: nitrogen in the spotlight

2021 ◽  
Author(s):  
Pierre-Mathieu Pélissier ◽  
Hans Motte ◽  
Tom Beeckman
Keyword(s):  
Signaling Pathways ◽  
Root System ◽  
Root Development ◽  
Lateral Root ◽  
Molecular Mechanisms ◽  
Root Formation ◽  
Lateral Roots ◽  
Nutrient Use Efficiency ◽  
Lateral Root Formation ◽  
Lateral Root Development

Abstract Lateral roots are important to forage for nutrients due to their ability to increase the uptake area of a root system. Hence, it comes as no surprise that lateral root formation is affected by nutrients or nutrient starvation, and as such contributes to the root system plasticity. Understanding the molecular mechanisms regulating root adaptation dynamics towards nutrient availability is useful to optimize plant nutrient use efficiency. There is at present a profound, though still evolving, knowledge on lateral root pathways. Here, we aimed to review the intersection with nutrient signaling pathways to give an update on the regulation of lateral root development by nutrients, with a particular focus on nitrogen. Remarkably, it is for most nutrients not clear how lateral root formation is controlled. Only for nitrogen, one of the most dominant nutrients in the control of lateral root formation, the crosstalk with multiple key signals determining lateral root development is clearly shown. In this update, we first present a general overview of the current knowledge of how nutrients affect lateral root formation, followed by a deeper discussion on how nitrogen signaling pathways act on different lateral root-mediating mechanisms for which multiple recent studies yield insights.

scholarly journals Relationship Between Root and Yield Related Morphological Characters in Pea (Pisum Sativum L.)

2014 ◽  
Vol 66 (1) ◽  
pp. 3-15
Author(s):  
Sylwia Ciaglo-Androsiuk
Keyword(s):  
Root System ◽  
Morphological Traits ◽  
Lateral Roots ◽  
Morphological Characters ◽  
Yield Potential ◽  
Cultivated Plants ◽  
Genetic Determination ◽  
Canonical Correlations ◽  
Effect On Yield

AbstractRelation between morphological traits of the root system and yield related traits is an important issue concerning efforts aiming at improving of ideotype of cultivated plants species, including pea. In this paper, to analyse the dependency between traits describing the root system morphology and yield potential, Person’s andSpearman's_correlations as well as canonical correlations were used.Root system was analyzed in 14 and 21 day-old seedlings growing in blotting-paper cylinders. Yield potential of pea was analysed in a field experiment. Results of Person’s and Spearman's_correlations revealed that number of lateral roots and lateral roots density were correlated witch yield related traits. Correlation between root length and shoot length was observed only for 14 day-old seedlings. The result of canonical correlations revealed that number of lateral roots and lateral roots density had the largest effect on yield related traits. This work highlights, that in order to improve the yield of pea it might become necessary to understand genetic determination of morphological traits of the root system, especially number of lateral roots.

Production of hydroxy fatty acids in the seeds of Arabidopsis thaliana

2000 ◽  
Vol 28 (6) ◽  
pp. 947-950 ◽  
Author(s):  
M. Smith ◽  
H. Moon ◽  
L. Kunst
Keyword(s):  
Fatty Acids ◽  
Arabidopsis Thaliana ◽  
Seed Oil ◽  
Desaturase Activity ◽  
Hydroxy Fatty Acids ◽  
Specific Expression ◽  
Oleate Desaturase ◽  
Arabidopsis Mutant ◽  
Mutant Lines ◽  
Seed Specific Expression

Seed-specific expression in Arabidopsis thaliana of oleate hydroxylase enzymes from castor bean and Lesquerella fendleri resulted in the accumulation of hydroxy fatty acids in the seed oil. By using various Arabidopsis mutant lines it was shown that the endoplasmic reticulum (ER) n–-3 desaturase (FAD3) and the FAE1 condensing enzyme are involved in the synthesis of polyunsaturated and very-long-chain hydroxy fatty acids, respectively. In Arabidopsis plants with an active ER Δ12-oleate desaturase the presence of hydroxy fatty acids corresponded to an increase in the levels of 18:1 and a decrease in 18:2 levels. Expression in yeast indicates that the castor hydroxylase also has a low level of desaturase activity.

scholarly journals Phosphorus and nitrogen interaction: loss of QC identity in response to P or N limitation is antecipated in pdr23 mutant

2011 ◽  
Vol 23 (3) ◽  
pp. 219-229 ◽  
Author(s):  
Cibele T. Costa ◽  
Mércio L. Strieder ◽  
Stephen Abel ◽  
Carla A. Delatorre
Keyword(s):  
Nucleic Acids ◽  
Lateral Roots ◽  
Adaptive Strategy ◽  
Quiescent Center ◽  
Wild Type ◽  
Short Root ◽  
Alternative Source ◽  
Low Phosphorus ◽  
Determinate Growth ◽  
Arabidopsis Mutant

Changes in root architecture are an important adaptive strategy used by plants in response to limited nutrient availability to increase the odds of acquiring them. The quiescent center (QC) plays an important role by altering the meristem activity causing differentiation and therefore, inducing a determinate growth program. The arabidopsis mutant pdr23 presents primary short root in the presence of nitrate and is inefficient in the use of nucleic acids as a source of phosphorus. In this study the effect of the pdr23 mutation on the QC maintenance under low phosphorus (P) and/or nitrogen is evaluated. QC identity is maintained in wild-type in the absence of nitrate and/or phosphate if nucleic acids can be used as an alternative source of these nutrients, but not in pdr23. The mutant is not able to use nucleic acids efficiently for substitute Pi, determinate growth is observed, similar to wild-type in the total absence of P. In the absence of N pdr23 loses the expression of QC identity marker earlier than wild-type, indicating that not only the response to P is altered, but also to N. The data suggest that the mutation affects a gene involved either in the crosstalk between these nutrients or in a pathway shared by both nutrients limitation response. Moreover loss of QC identity is also observed in wild-type in the absence of N at longer limitation. Less drastic symptoms are observed in lateral roots of both genotypes.

scholarly journals AT-HOOK MOTIF NUCLEAR LOCALISED PROTEIN 18 as a Novel Modulator of Root System Architecture

2020 ◽  
Author(s):  
Marek Šírl ◽  
Tereza Šnajdrová ◽  
Dolores Gutiérrez-Alanís ◽  
Joseph G. Dubrovsky ◽  
Jean Phillipe Vielle-Calzada ◽  
...  
Keyword(s):  
Root System ◽  
System Architecture ◽  
Lateral Root ◽  
Apical Meristem ◽  
Lateral Roots ◽  
Primary Root ◽  
Root System Architecture ◽  
Root Apical Meristem ◽  
Root Initiation ◽  
Lateral Root Initiation

The AT-HOOK MOTIF NUCLEAR LOCALIZED PROTEIN (AHL) gene family encodes embryophyte-specific nuclear proteins with DNA binding activity. They modulate gene expression and affect various developmental processes in plants. We identify AHL18 (At3G60870) as a developmental modulator of root system architecture and growth. AHL18 regulates the length of the proliferation domain and number of dividing cells in the root apical meristem and thereby, cell production. Both primary root growth and lateral root development respond according to AHL18 transcription level. The ahl18 knock-out plants show reduced root systems due to a shorter primary root and a lower number of lateral roots. This change results from a higher number of arrested and non-developing lateral root primordia (LRP) rather than from decreased initiation. Overexpression of AHL18 results in a more extensive root system, longer primary roots, and increased density of lateral root initiation events. Formation of lateral roots is affected during the initiation of LRP and later development. AHL18 regulate root apical meristem activity, lateral root initiation and emergence, which is in accord with localization of its expression.

scholarly journals Stability Analysis of Ecological Slopes Based on a 3D Finite Element Model

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
ZiFan Sui ◽  
Weijia Yuan ◽  
Wen Yi ◽  
Weihuan Yang
Keyword(s):  
Finite Element ◽  
Root System ◽  
Safety Factor ◽  
Cynodon Dactylon ◽  
Plant Protection ◽  
Lateral Roots ◽  
Plant Distribution ◽  
Plant Roots ◽  
Research Subjects ◽  
Finite Element Software

To explore the effect of grass and shrub plant roots on the stability of soil slopes in rainy areas in the south, this article relies on the Longlang Expressway construction project. Cynodon dactylon and Magnolia multiflora were selected as research subjects. The plant distribution characteristics and mechanical properties are analyzed. This paper uses ABAQUS finite element software to construct a 3D model of the planted slope in the test section. The stress and strain on the root system and the soil were observed, and the variation law of slope stability before and after plant protection under different rainfall events was compared and analyzed. The test and simulation results show that the root content of Cynodon dactylon gradually decreases with increasing depth. Cynodon dactylon was mainly distributed in the 0–30 cm soil body, and its effect on improving the cohesion of the soil body reached 75%. Magnolia multiflora belongs to vertical roots and has a strong and longer main root with relatively developed lateral roots. Its root system passes through the sliding surface of the slope bottom, which reduces the maximum equivalent plastic stress generated inside the slope by 61%. When the total rainfall duration is unchanged, under the three rainfall intensities of small, medium, and large, herbaceous plants increase the safety factor of the soil by 1.33%, 2.08%, and 6.1%, respectively, and the roots of shrubs increase the safety factor of the soil by 3.29%, 4.08%, and 4.32%, respectively. When the rainfall intensity does not change, as the rainfall time increases, the effect of plants on the slope safety factor first gradually increases and eventually stabilizes. The research results provide a reliable theoretical basis for analyzing the effect of plant roots on soil consolidation and slope protection, and they also lay a technical foundation for the promotion and application of ecological slope protection technology.

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