scholarly journals Expression analysis of alpha-tubulin genes during cold acclimation in winter wheat Demetra

1970 ◽  
Vol 21 ◽  
pp. 107-111
Author(s):  
D. D. Bui ◽  
A. Ye. Demkovych ◽  
Ya. V. Pirko ◽  
Ya. B. Blume
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Cold Acclimation ◽  
Resistance Mechanism ◽  
Triticum Aestivum L ◽  
Expression Level ◽  
Alpha Tubulin ◽  
Tubulin Genes ◽  
Vital Functions ◽  
Cytoskeleton Reorganization

Aim. Wheat (Triticum aestivum L.) display significant increase in freezing tolerance during a period of cold acclimation (CA). Functions and regulations unraveling of CA-associated genes can help in cold-resistant cultivars receiving using biotechnology methods. Cytoskeleton reorganization is an important element of the cold resistance mechanism associated with tubulin expression level alterations. Methods. Cold acclimation during 28 days was carried out. The expression level of 15 members of alpha-tubulin genes was measured using RT Real-time PCR with specific primers. Results. Similar patterns of expression alterations were observed within subfamilies. The highest levels of expression were recorded for the fourth subfamily members (Tuba_4-1, 4-2, 4-3) and Tuba_2-3 gene. These 4 alpha-tubulin genes may be involved in the vital functions maintenance during the first days of the low temperature influence. Conclusions. It is likely that four of the fifteen genes are related to cold tolerance mechanisms since they have significantly higher expression levels during cold acclimation. Keywords: Triticum aestivum, winter wheat, tubulin, cold acclimation.

scholarly journals Naturally Occurring Ecdysteroids in Triticum aestivum L. and Evaluation of Fenarimol as a Potential Inhibitor of Their Biosynthesis in Plants

2021 ◽  
Vol 22 (6) ◽  
pp. 2855
Author(s):  
Anna Janeczko ◽  
Jana Oklestkova ◽  
Danuše Tarkowská ◽  
Barbara Drygaś
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Triticum Aestivum L ◽  
Growth Conditions ◽  
Animal Kingdom ◽  
Experimental Conditions ◽  
P450 Monooxygenase ◽  
Naturally Occurring ◽  
Regulatory Effects ◽  
Potential Use

Ecdysteroids (ECs) are steroid hormones originally found in the animal kingdom where they function as insect molting hormones. Interestingly, a relatively high number of these substances can also be formed in plant cells. Moreover, ECs have certain regulatory effects on plant physiology, but their role in plants still requires further study. One of the main aims of the present study was to verify a hypothesis that fenarimol, an inhibitor of the biosynthesis of ECs in the animal kingdom, also affects the content of endogenous ECs in plants using winter wheat Triticum aestivum L. as a model plant. The levels of endogenous ECs in winter wheat, including the estimation of their changes during a course of different temperature treatments, have been determined using a sensitive analytical method based on UHPLC-MS/MS. Under our experimental conditions, four substances of EC character were detected in the tissue of interest in amounts ranging from less than 1 to over 200 pg·g−1 FW: 20-hydroxyecdysone, polypodine B, turkesterone, and isovitexirone. Among them, turkesterone was observed to be the most abundant EC and accumulated mainly in the crowns and leaves of wheat. Importantly, the level of ECs was observed to be dependent on the age of the plants, as well as on growth conditions (especially temperature). Fenarimol, an inhibitor of a cytochrome P450 monooxygenase, was shown to significantly decrease the level of naturally occurring ECs in experimental plants, which may indicate its potential use in studies related to the biosynthesis and physiological function of these substances in plants.

Cirsium arvense (L.) Scop. competition with winter wheat (Triticum aestivum L.)

Weed Research ◽  
1991 ◽  
Vol 31 (6) ◽  
pp. 409-415 ◽  
Author(s):  
B. R. MCLENNAN ◽  
R. ASHFORD ◽  
M. D. DEVINE
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Triticum Aestivum L ◽  
Cirsium Arvense

scholarly journals Long-Term Winter Wheat (Triticum aestivum L.) Seasonal Irrigation Amount, Evapotranspiration, Yield, and Water Productivity under Semiarid Climate

Agronomy ◽  
2018 ◽  
Vol 8 (6) ◽  
pp. 96 ◽  
Author(s):  
Koffi Djaman ◽  
Michael O’Neill ◽  
Curtis Owen ◽  
Daniel Smeal ◽  
Margaret West ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Water Productivity ◽  
Triticum Aestivum L ◽  
Irrigation Amount ◽  
Semiarid Climate ◽  
Seasonal Irrigation
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