scholarly journals Methylation of human elongation factor eEF1A2 is not essential for eEF1A2-eEF1B interaction

2020 ◽  
Vol 36 (4) ◽  
pp. 254-263
Author(s):  
L. V. Porubleva ◽  
D. L. Kolesnyk ◽  
A. V. El’skaya ◽  
B. S. Negrutskii
Keyword(s):  
Elongation Factor ◽  
Human Elongation Factor

scholarly journals Chromosomal assignment of the gene for human elongation factor 2.

1984 ◽  
Vol 81 (10) ◽  
pp. 3158-3162 ◽  
Author(s):  
Y. Kaneda ◽  
M. C. Yoshida ◽  
K. Kohno ◽  
T. Uchida ◽  
Y. Okada
Keyword(s):  
Elongation Factor ◽  
Chromosomal Assignment ◽  
Elongation Factor 2 ◽  
Human Elongation Factor

scholarly journals Solution Structure of the 162 Residue C-terminal Domain of Human Elongation Factor 1Bγ

2003 ◽  
Vol 278 (44) ◽  
pp. 43443-43451 ◽  
Author(s):  
Sophie Vanwetswinkel ◽  
Jan Kriek ◽  
Gregers R. Andersen ◽  
Peter Güntert ◽  
Jan Dijk ◽  
...  
Keyword(s):  
Solution Structure ◽  
Elongation Factor ◽  
Terminal Domain ◽  
Human Elongation Factor

Construction of a Plasmid Containing the Complete Coding Region of Human Elongation Factor 2

1992 ◽  
Vol 373 (1) ◽  
pp. 201-204 ◽  
Author(s):  
Jozef HANES ◽  
Johannes FREUDENSTEIN ◽  
Georg RAPP ◽  
Karl Heinz SCHEIT
Keyword(s):  
Elongation Factor ◽  
Coding Region ◽  
Elongation Factor 2 ◽  
Human Elongation Factor

scholarly journals Purification and characterization of native human elongation factor 2

2019 ◽  
Vol 158 ◽  
pp. 15-19
Author(s):  
Rasmus Kock Flygaard ◽  
Beatrice Malacrida ◽  
Patrick Kiely ◽  
Lasse Bohl Jenner
Keyword(s):  
Elongation Factor ◽  
Purification And Characterization ◽  
Elongation Factor 2 ◽  
Human Elongation Factor

Human Elongation Factor EF-1β: Cloning and Characterization of the EF1β5a Gene and Assignment of EF-1β Isoforms to Chromosomes 2, 5, 15, and X

1993 ◽  
Vol 197 (1) ◽  
pp. 154-162 ◽  
Author(s):  
A. Pizzuti ◽  
M. Gennarelli ◽  
G. Novelli ◽  
A. Colosimo ◽  
S. Locicero ◽  
...  
Keyword(s):  
Elongation Factor ◽  
Human Elongation Factor

Assignment of Human Elongation Factor 1α Genes:EEF1AMaps to Chromosome 6q14 andEEF1A2to 20q13.3

Genomics ◽  
1996 ◽  
Vol 36 (2) ◽  
pp. 359-361 ◽  
Author(s):  
A. Lund ◽  
S.M. Knudsen ◽  
H. Vissing ◽  
B. Clark ◽  
N. Tommerup
Keyword(s):  
Elongation Factor ◽  
Elongation Factor 1Α ◽  
Human Elongation Factor

scholarly journals In Vitro and In Vivo Characterization of a Dual-Function Green Fluorescent Protein–HSV1-Thymidine Kinase Reporter Gene Driven by the Human Elongation Factor 1α Promoter

2002 ◽  
Vol 1 (2) ◽  
pp. 153535002002011
Author(s):  
Gary D. Luker ◽  
Kathryn E. Luker ◽  
Vijay Sharma ◽  
Christina M. Pica ◽  
Julie L. Dahlheimer ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Reporter Gene ◽  
Fluorescent Protein ◽  
Elongation Factor ◽  
Dual Function ◽  
Elongation Factor 1Α ◽  
Green Fluorescent ◽  
Human Elongation Factor

Toward the goal of monitoring activity of native mammalian promoters with molecular imaging techniques, we stably transfected DU145 prostate carcinoma cells with a fusion construct of enhanced green fluorescent protein (EGFP) and wild-type herpes simplex virus-1 thymidine kinase (HSV1-TK) as a reporter gene driven by the promoter for human elongation factor 1α (EF-1α-EGFP-TK). Using this model system, expression of EGFP was quantified by flow cytometry and fluorescence microscopy, while the HSV1-TK component of the reporter was quantified with 8-[3H]ganciclovir (8-[3H]GCV). As analyzed by flow cytometry, passage of EGFP-TK-DU145 transfected cells (ETK) in vitro resulted in populations of cells with high and low expression of EGFP over time. High and low ETK cells retained 23-fold and 5-fold more GCV, respectively, than control. While differences in uptake and retention of GCV corresponded to relative expression of the reporter gene in each subpopulation of cells as determined by both flow cytometry (EGFP) and quantitative RT-PCR, the correlation was not linear. Furthermore, in high ETK cells, net retention of various radiolabeled nucleoside analogues varied; the rank order was 8-[3H]GCV < 9-(4-fluoro-3-hydroxymethylbutyl)guanine ([18F]FHBG) ≈ 8-[3H]penciclovir (8-[3H]PCV) < 2′-fluoro-2′-deoxy-5-iodouracil-beta-d-arabinofuranoside (2-[14C]FIAU). Xenograft tumors of ETK cells in vivo accumulated 2.5-fold more 8-[3H]GCV per gram of tissue and showed greater fluorescence from EGFP than control DU145 cells, demonstrating that the reporter gene functioned in vivo. These data extend previous reports by showing that a human promoter can be detected in vitro and in vivo with a dual-function reporter exploiting optical and radiotracer techniques.

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