scholarly journals Mice with pituitary tumor transforming gene (pttg) knockout demonstrate increased urinary space in renal corpuscles

2014 ◽  
Vol 30 (2) ◽  
pp. 122-128 ◽  
Author(s):  
O. Yu. Varyvoda ◽  
A. M. Yashchenko ◽  
A. D. Lutsyk ◽  
R. O. Bilyy ◽  
S. V. Afanasiev ◽  
...  
Keyword(s):  
Pituitary Tumor ◽  
Pituitary Tumor Transforming Gene ◽  
Transforming Gene ◽  
Urinary Space

scholarly journals Thyroid Hormone Receptors Suppress Pituitary Tumor Transforming Gene 1 Activity in Hepatoma

2008 ◽  
Vol 68 (6) ◽  
pp. 1697-1706 ◽  
Author(s):  
Ruey-Nan Chen ◽  
Ya-Hui Huang ◽  
Chau-Ting Yeh ◽  
Chen-Hsin Liao ◽  
Kwang-Huei Lin
Keyword(s):  
Thyroid Hormone ◽  
Pituitary Tumor ◽  
Hormone Receptors ◽  
Thyroid Hormone Receptors ◽  
Pituitary Tumor Transforming Gene ◽  
Transforming Gene

scholarly journals Pituitary tumor transforming gene: a novel therapeutic target for glioma treatment

2015 ◽  
Vol 47 (6) ◽  
pp. 414-421 ◽  
Author(s):  
L. Cui ◽  
S. Xu ◽  
Z. Song ◽  
G. Zhao ◽  
X. Liu ◽  
...  
Keyword(s):  
Therapeutic Target ◽  
Pituitary Tumor ◽  
Pituitary Tumor Transforming Gene ◽  
Transforming Gene ◽  
Novel Therapeutic

scholarly journals Overexpression of pituitary tumor transforming gene 1 in HCC is associated with angiogenesis and poor prognosis

Hepatology ◽  
2006 ◽  
Vol 43 (6) ◽  
pp. 1267-1275 ◽  
Author(s):  
Tsutomu Fujii ◽  
Shuji Nomoto ◽  
Katsumi Koshikawa ◽  
Yasushi Yatabe ◽  
Osamu Teshigawara ◽  
...  
Keyword(s):  
Pituitary Tumor ◽  
Poor Prognosis ◽  
Pituitary Tumor Transforming Gene ◽  
Transforming Gene

scholarly journals Overexpressed Pituitary Tumor-Transforming Gene Causes Aneuploidy in Live Human Cells

Endocrinology ◽  
2003 ◽  
Vol 144 (11) ◽  
pp. 4991-4998 ◽  
Author(s):  
Run Yu ◽  
Wenge Lu ◽  
Jiandong Chen ◽  
Chris J. McCabe ◽  
Shlomo Melmed
Keyword(s):  
Cancer Cells ◽  
Chromosome Segregation ◽  
Pituitary Tumor ◽  
Fluorescent Protein ◽  
Human Cancer ◽  
Live Cells ◽  
Pituitary Tumor Transforming Gene ◽  
Transforming Gene

Abstract The mammalian securin, pituitary tumor-transforming gene (PTTG), is overexpressed in several tumors and transforms cells in vitro and in vivo. To test the hypothesis that PTTG overexpression causes aneuploidy, enhanced green fluorescent protein (EGFP)-tagged PTTG (PTTG-EGFP) was expressed in human H1299 cancer cells (with undetectable endogenous PTTG expression) and mitosis of individual live cells observed. Untransfected cells and cells expressing EGFP alone exhibited appropriate mitosis. PTTG-EGFP markedly prolonged prophase and metaphase, indicating that PTTG blocks progression of mitosis to anaphase. In cells that underwent apparently normal mitosis (35 of 65 cells), PTTG-EGFP was degraded about 1 min before anaphase onset. Cells that failed to degrade PTTG-EGFP exhibited asymmetrical cytokinesis without chromosome segregation (18 of 65 cells) or chromosome decondensation without cytokinesis (9 of 65 cells), resulting in appearance of a macronucleus. Fifty-one of 55 cells expressing a nondegradable mutant PTTG exhibited asymmetrical cytokinesis without chromosome segregation, and some (4 of 55) decondensed chromosomes, both resulting in macronuclear formation. During this abnormal cytokinesis, all chromosomes and spindles and both centrosomes moved to one daughter cell, suggesting potential chaos in the subsequent mitosis. In conclusion, failure of PTTG degradation or enhanced PTTG accumulation, as a consequence of overexpression, inhibits mitosis progression and chromosome segregation but does not directly affect cytokinesis, resulting in aneuploidy. These results demonstrate that PTTG induces aneuploidy in single, live, human cancer cells.

scholarly journals Murine pituitary tumor-transforming gene functions as a securin protein in insulin-secreting cells

2006 ◽  
Vol 191 (1) ◽  
pp. 45-53 ◽  
Author(s):  
Run Yu ◽  
Martha Cruz-Soto ◽  
Sergio Li Calzi ◽  
Hongxiang Hui ◽  
Shlomo Melmed
Keyword(s):  
Cell Proliferation ◽  
Pituitary Tumor ◽  
Cell Cycle Progression ◽  
Fluorescent Protein ◽  
Cell System ◽  
Β Cell ◽  
Human Pituitary ◽  
Pituitary Tumor Transforming Gene ◽  
Expression Levels ◽  
Transforming Gene

Human pituitary tumor-transforming gene 1 (PTTG1) encodes a securin protein critically important in regulating chromosome separation. Murine PTTG (mPTTG) is 66% homologous to human PTTG1 and PTTG-null (PTTG−/−) mice exhibit pancreatic β-cell hypoplasia and abnormal nuclear morphology with resultant diabetes. As we show that ductal β-cell neogenesis is intact in PTTG−/− mice, we explored mechanism for defective β-cell replication. We tested whether mPTTG exhibits securin properties in mouse insulin-secreting insulinoma MIN6 cells, using a live-cell system to monitor mitosis in cells transfected with an enhanced green fluorescent protein (EGFP)-tagged mPTTG conjugate (mPTTG-EGFP). To fulfill the criteria for securin properties, the protein should undergo degradation immediately before the metaphase-to-anaphase transition when expression levels are low, and should inhibit metaphase-to-anaphase transition when expression levels are high. EGFP itself did not undergo degradation throughout mitosis and high levels of EGFP per se did not affect normal mitosis progression (n=25). However, mPTTG-EGFP was degraded 2 min before the metaphase-to-anaphase transition when expression levels were low (n=19), and high mPTTG-EGFP levels blocked metaphase-to-anaphase transition in 13 cells. mPTTG-EGFP inhibited MIN6 cell proliferation and caused apoptosis. Immunocoprecipitation demonstrated binding of mPTTG-EGFP and separase. These results show that mPTTG exhibits properties consistent with a murine securin in insulin-secreting mouse cells and mPTTG overexpression inhibits cell proliferation, suggesting that defective β-cell proliferation observed in PTTG−/− mice is likely due to abnormal cell-cycle progression.

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