scholarly journals Interaction of cyanine dyes with nucleic acids. New (pyrido)(thio)trimethincyanine dye CCyan 40 for fluorescent labeling of oligonucleotides

2002 ◽  
Vol 18 (4) ◽  
pp. 340-346 ◽  
Author(s):  
S. M. Yarmoluk ◽  
M. Yu. Losytskyy ◽  
V. B. Kovatska ◽  
S. M. Tomachynski ◽  
T. Yu. Ogul'chansky ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Cyanine Dyes ◽  
Fluorescent Labeling

Synthesis and study of nucleic acids interactions of novel monomethine cyanine dyes

2012 ◽  
Vol 92 (3) ◽  
pp. 1184-1191 ◽  
Author(s):  
Stefka Kaloyanova ◽  
Ivo Crnolatac ◽  
Nedyalko Lesev ◽  
Ivo Piantanida ◽  
Todor Deligeorgiev
Keyword(s):  
Nucleic Acids ◽  
Cyanine Dyes

scholarly journals Label-free horizontal EMSA for analysis of protein-RNA interactions

2019 ◽  
Author(s):  
William Perea ◽  
Nancy L. Greenbaum
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Dissociation Constant ◽  
Intermolecular Interactions ◽  
Ethidium Bromide ◽  
Fluorescent Labeling ◽  
Label Free ◽  
Cationic Protein ◽  
Intercalating Dye ◽  
Protein Components

AbstractWe describe a method to analyze the affinity and specificity of interactions between proteins and RNA using horizontal PAGE under non-denaturing conditions. The method permits tracking of migration of anionic and cationic biomolecules and complexes toward anode and cathode, respectively, therefore enabling quantification of bound and free biomolecules of different charges and affinity of their intermolecular interactions. The gel is stained with a fluorescent intercalating dye (SYBR®Gold or ethidium bromide) for visualization of nucleic acids followed by Coomassie® Brilliant Blue R-250 for visualizations of proteins; the dissociation constant is determined separately from the intensity of unshifted and shifted bands visualized by each dye. The method permits calculation of bound and unbound anionic nucleic acid and cationic protein components in the same gel, regardless of charge, under identical conditions, and avoids the need for radioisotope or fluorescent labeling of either component.

scholarly journals Interaction of cyanine dyes with nucleic acids. Meso-substituted trimethincyanines for fluorescent detection of nucleic acids

2002 ◽  
Vol 18 (3) ◽  
pp. 243-252 ◽  
Author(s):  
S. S. Lukashov ◽  
M. Yu. Losytskyy ◽  
Yu. P. Kovtun ◽  
S. M. Yarmoluk
Keyword(s):  
Nucleic Acids ◽  
Cyanine Dyes ◽  
Fluorescent Detection

Homodimeric monomethine cyanine dyes SOSO-1 and TOTO-1-6C—synthesis and fluorescence properties in the presence of nucleic acids

2004 ◽  
Vol 61 (1) ◽  
pp. 79-84 ◽  
Author(s):  
Todor Deligeorgiev ◽  
Iliana Timcheva ◽  
Vera Maximova ◽  
Nikolai Gadjev ◽  
Alexey Vassilev ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Cyanine Dyes ◽  
Fluorescence Properties

Interaction of cyanine dyes with nucleic acids. XXI. Arguments for half-intercalation model of interaction

Biopolymers ◽  
2001 ◽  
Vol 62 (4) ◽  
pp. 219-227 ◽  
Author(s):  
S. M. Yarmoluk ◽  
S. S. Lukashov ◽  
T. Yu. Ogul'Chansky ◽  
M. Yu. Losytskyy ◽  
O. S. Kornyushyna
Keyword(s):  
Nucleic Acids ◽  
Cyanine Dyes
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