scholarly journals Conformation of the OR3 operator of phage λ and its fragment in aqueous and aqueous-trifluoroethanol solutions

1986 ◽  
Vol 2 (2) ◽  
pp. 101-106 ◽  
Author(s):  
V. I. Ivanov ◽  
L. E. Minchenkova ◽  
A. K. Shchelkina ◽  
B. K. Chernov ◽  
A. P. Yartsev ◽  
...  
Keyword(s):  
Phage Lambda ◽  
Or3 Operator

scholarly journals VERY SHORT PATCH MISMATCH REPAIR IN PHAGE LAMBDA: REPAIR SITES AND LENGTH OF REPAIR TRACTS

Genetics ◽  
1986 ◽  
Vol 114 (4) ◽  
pp. 1041-1060
Author(s):  
M Lieb ◽  
E Allen ◽  
D Read
Keyword(s):  
Mismatch Repair ◽  
Bacteriophage Λ ◽  
Phage Lambda ◽  
High Frequencies ◽  
Vsp Repair

ABSTRACT Five amber mutations in the repressor (cl) gene of bacteriophage λ recombine anomalously with nearby cl mutations. When any of these markers is used in four-factor crosses, cl  + recombinants that are expected to require three crossovers occur at high frequencies. These recombinants are attributable to very-short-patch (VSP) repair of specific mismatches in DNA heteroduplexes formed during recombination between the markers flanking cl. The sites of the repairprone mutations and the lengths of repair tracts have now been determined. Amber mutations subject to VSP repair are C to T transitions in (see PDF), the sequence methylated by the product of gene dcm, and also in the related 5′CAGG or 5′CCAG sequences. Ambers arising in CAG sequences found in other contexts, or in codons other than CAG, were not subject to VSP repair. Repair tracts rarely, if ever, exceed ten nucleotides in length, and can be as short as two nucleotides. A repair-prone mutation does not stimulate recombination between flanking cl markers.

scholarly journals Phage genetic sites involved in lambda growth inhibition by the Escherichia coli rap mutant.

Genetics ◽  
1989 ◽  
Vol 121 (3) ◽  
pp. 401-409
Author(s):  
P Guzmán ◽  
G Guarneros
Keyword(s):  
Escherichia Coli ◽  
Growth Inhibition ◽  
Phage Genome ◽  
Bacteriophage Lambda ◽  
Phage Lambda ◽  
Wild Type ◽  
Attp Site

Abstract The rap mutation of Escherichia coli prevents the growth of bacteriophage lambda. We have isolated phage mutants that compensate for the host deficiency. The mutations, named bar, were genetically located to three different loci of the lambda genome: barI in the attP site, barII in the cIII ea10 region, and barIII within or very near the imm434 region. The level of lambda leftward transcription correlates with rap exclusion. Phage lambda mutants partially defective in the pL promoter or in pL-transcript antitermination showed a Bar- phenotype. Conversely, mutants constitutive for transcription from the pI or pL promoters were excluded more stringently by rap bacteria. We conclude that rap exclusion depends on the magnitude of transcription through the wild type bar loci in the phage genome.

3'-End formation at the phage .lambda. tR1 .rho.-dependent transcription termination site

Biochemistry ◽  
1991 ◽  
Vol 30 (22) ◽  
pp. 5429-5437 ◽  
Author(s):  
Elizabeth A. Roberts ◽  
Theresa L. Eisenbraun ◽  
Christopher L. Andrews ◽  
David G. Bear
Keyword(s):  
Transcription Termination ◽  
Phage Lambda ◽  
Transcription Termination Site
Sign in / Sign up

Export Citation Format

Share Document