scholarly journals Functional role of C-terminal domain of Thermus thermophilus leucyl-tRNA synthetase

2010 ◽  
Vol 26 (6) ◽  
pp. 478-485 ◽  
Author(s):  
O. I. Gudzera ◽  
A. D. Yaremchuk ◽  
M. A. Tukalo
Keyword(s):  
Functional Role ◽  
Thermus Thermophilus ◽  
Trna Synthetase ◽  
Terminal Domain

scholarly journals Functional role of the N-terminal domain of ΔFosB in response to stress and drugs of abuse

Neuroscience ◽  
2015 ◽  
Vol 284 ◽  
pp. 165-170 ◽  
Author(s):  
Y.N. Ohnishi ◽  
Y.H. Ohnishi ◽  
V. Vialou ◽  
E. Mouzon ◽  
Q. LaPlant ◽  
...  
Keyword(s):  
Functional Role ◽  
Drugs Of Abuse ◽  
Terminal Domain ◽  
Response To Stress

Functional role of Aspergillus oryzae glucoamylase C-terminal domain investigated using its cDNA

1995 ◽  
Vol 80 (3) ◽  
pp. 280-282 ◽  
Author(s):  
Tadashi Nagashima ◽  
Yutaka Yamamoto ◽  
Katsuhiko Kitamoto ◽  
Chieko Kumagai
Keyword(s):  
Aspergillus Oryzae ◽  
Functional Role ◽  
Terminal Domain

scholarly journals Critical Role of Helix 4 of HIV-1 Capsid C-terminal Domain in Interactions with Human Lysyl-tRNA Synthetase

2007 ◽  
Vol 282 (44) ◽  
pp. 32274-32279 ◽  
Author(s):  
Brandie J. Kovaleski ◽  
Robert Kennedy ◽  
Ahmad Khorchid ◽  
Lawrence Kleiman ◽  
Hiroshi Matsuo ◽  
...  
Keyword(s):  
Critical Role ◽  
Trna Synthetase ◽  
Terminal Domain ◽  
Hiv 1

scholarly journals A functional role of the glycosylated N-terminal domain of chondromodulin-I

2010 ◽  
Vol 29 (1) ◽  
pp. 23-30 ◽  
Author(s):  
Jun Kondo ◽  
Hiroyuki Shibata ◽  
Shigenori Miura ◽  
Akira Yamakawa ◽  
Koji Sato ◽  
...  
Keyword(s):  
Functional Role ◽  
Terminal Domain

scholarly journals Functional role of the C-terminal domain of Bestrophin-1, a calcium-activated chloride channel

IBRO Reports ◽  
2019 ◽  
Vol 6 ◽  
pp. S398-S399
Author(s):  
Dong-Hyun Kim ◽  
Junmo Hwang ◽  
Hyun-Ho Lim
Keyword(s):  
Chloride Channel ◽  
Functional Role ◽  
Terminal Domain

scholarly journals Crystal structures of GCN2 C-terminal domain: Insight into GCN2 regulation

2014 ◽  
Vol 70 (a1) ◽  
pp. C1407-C1407
Author(s):  
Isha Singh ◽  
Hongzhen He ◽  
Sheree Wek ◽  
Souvik Dey ◽  
Thomas Baird ◽  
...  
Keyword(s):  
Amino Acid ◽  
Crystal Structures ◽  
Kinase Domain ◽  
Trna Synthetase ◽  
Amino Acid Starvation ◽  
Nucleic Acid Binding ◽  
Serine Threonine Kinase ◽  
Functional Studies ◽  
Terminal Domain

General control non-derepressible 2 kinase (GCN2) is a serine threonine kinase that curtails translation in response to diverse stress stimuli [1]. It is a primary sensor of amino acid starvation and mediates translation repression by phosphorylating eIF2 [2]. In addition to the kinase domain, GCN2 contains two regulatory regions; a histidyl-tRNA synthetase-like domain (HisRS) and a C-terminal domain (CTD), which function together to sense nutrient depletion. Both domains have been proposed to bind uncharged tRNA's that accumulate during amino acid starvation followed by dimerization of the kinase domain facilitating activation of GCN2 [3]. Thus, while the CTD plays an important regulatory role in activating GCN2, information on how the CTD facilitates dimerization and whether the CTD plays a similar role in murine GCN2 is limited. Moreover, the sequences of vertebrate CTDs share less than 10% sequence identity with their yeast counterpart; therefore, it is not known whether regulatory mechanisms in GCN2 are conserved across different species. We present here the experimentally phased crystal structures of murine CTD at 1.9 Å and yeast CTD at 1.95 Å. Both murine and yeast CTDs share a novel interdigitated dimeric organization, although the dimeric structures differ somewhat in overall shape and size. Additional biochemical analysis of the murine CTD confirms an important role for dimerization in its activation. Moreover, functional studies reveal that both yeast and murine GCN2 have similar nucleic acid binding properties, but mGCN2 does not appear to exhibit ribosomal association, a key feature in the model for regulation of yeast GCN2, suggesting that there are regulatory differences between the murine GCN2 and its yeast counterpart. Our data provides a basis for understanding the role of the CTD in regulation of GCN2 in both yeast and mammals.

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