scholarly journals 2',3'-dideoxy-3'-aminonucleoside-5'-triphosphates inhibit repair DNA synthesis in rat liver chromatin

1985 ◽  
Vol 1 (3) ◽  
pp. 125-131 ◽  
Author(s):  
A. A. Kraevsky ◽  
M. K. Kukhanova ◽  
L. A. Alexandrova ◽  
N. V. Belyakova ◽  
V. M. Krutyakov
Keyword(s):  
Dna Synthesis ◽  
Rat Liver ◽  
Liver Chromatin

scholarly journals Conformational changes in rat liver chromatin after liver regeneration

1981 ◽  
Vol 193 (3) ◽  
pp. 671-678 ◽  
Author(s):  
H Simpkins ◽  
L M Thompson ◽  
N Waldeck ◽  
D S Gross ◽  
D Mooney
Keyword(s):  
Dna Synthesis ◽  
Rat Liver ◽  
Conformational Changes ◽  
Histone H3 ◽  
Tryptophan Fluorescence ◽  
Chromatin Protein ◽  
Detectable Difference ◽  
Liver Chromatin ◽  
Triton X

N-Pyrenemaleimide, a fluorescent probe that specifically labels histone H3 of rat liver chromatin in situ, was used to monitor the accessibility of histone H3 in chromatin isolated from rat liver at different times during degeneration. At times of maximum DNA synthesis (18–24 h after hepatectomy), the accessibility of the probe was found to be markedly (40–50%) increased. This increase is abolished, however, by treatment of the chromatin fibres with high salt (2 M-NaCl) or detergent. Tryptophan fluorescence was also enhanced at points of maximum DNA synthesis, suggesting that some non-histone tryptophan-containing protein was being synthesized. The polarization of the labelled histone H3 is not markedly altered, suggesting that fibre aggregation or dissociation does not occur. Mononucleosomes extracted from sham-operated and hepatectomized animals did not exhibit any difference in binding to the probe. Also, analysis of the chromatin protein by electrophoresis on detergent- and acid/urea/ Triton-X-100-containing polyacrylamide gels showed no detectable difference in histone H3 : 1, H3 : 2 or H3 : 3 subclasses.

Purification and properties of a neutral protease from rat liver chromatin

Biochemistry ◽  
1974 ◽  
Vol 13 (25) ◽  
pp. 5128-5134 ◽  
Author(s):  
Ming Ta Chong ◽  
William T. Garrard ◽  
James Bonner
Keyword(s):  
Rat Liver ◽  
Neutral Protease ◽  
Purification And Properties ◽  
Liver Chromatin

DEOXYCYTIDYLATE DEAMINASE LEVELS IN REGENERATING RAT LIVER

1961 ◽  
Vol 39 (6) ◽  
pp. 1043-1054 ◽  
Author(s):  
D. K. Myers ◽  
C. Anne Hemphill ◽  
Constance M. Townsend
Keyword(s):  
Dna Synthesis ◽  
Liver Regeneration ◽  
Partial Hepatectomy ◽  
Rat Liver ◽  
Deoxyribonucleic Acid ◽  
Regenerating Liver ◽  
Regenerating Rat Liver ◽  
High Level ◽  
Early Regeneration

Deoxycytidylate deaminase activity and net synthesis of deoxyribonucleic acid (DNA) in vivo were found to increase at approximately the same time during the early stages of liver regeneration. However, deaminase activity in the regenerating liver remained at a high level for 1 day after DNA synthesis had slowed down again during the later stages of regeneration. The increase in deaminase activity was restricted as a result of exposure to 600 r X radiation during early regeneration, but this effect only became evident 11–16 hours after the irradiation. Irradiation on the second day after partial hepatectomy, when deaminase levels in control regenerating livers were relatively constant, failed to affect the deaminase activity immediately but did produce a 40–50% decrease in activity 11–16 hours later. Other antimitotic agents, e.g., colchicine, had little effect on deaminase activity.

Association of the thyroid hormone receptor with rat liver chromatin

Biochemistry ◽  
1981 ◽  
Vol 20 (24) ◽  
pp. 6781-6789 ◽  
Author(s):  
Donald B. Jump ◽  
Steven Seelig ◽  
Harold L. Schwartz ◽  
Jack H. Oppenheimer
Keyword(s):  
Thyroid Hormone ◽  
Rat Liver ◽  
Hormone Receptor ◽  
Thyroid Hormone Receptor ◽  
Liver Chromatin
Sign in / Sign up

Export Citation Format

Share Document