scholarly journals Electromechanical Conditioning of Adult Progenitor Cells Improves Recovery of Cardiac Function After Myocardial Infarction

2016 ◽  
Vol 6 (3) ◽  
pp. 970-981 ◽  
Author(s):  
Aida Llucià-Valldeperas ◽  
Carolina Soler-Botija ◽  
Carolina Gálvez-Montón ◽  
Santiago Roura ◽  
Cristina Prat-Vidal ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Cardiac Function ◽  
Progenitor Cells

scholarly journals MicroRNA-326-5p enhances therapeutic potential of endothelial progenitor cells for myocardial infarction

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Xiaoting Li ◽  
Xiang Xue ◽  
Yuejun Sun ◽  
Lei Chen ◽  
Ting Zhao ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Cardiac Function ◽  
Western Blot ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Tube Formation ◽  
Luciferase Reporter Assay ◽  
Luciferase Reporter ◽  
Reporter Assay

Abstract Background Our study sought to investigate the therapeutic effects and mechanisms of miR-326-5p-overexpressing endothelial progenitor cells (EPCs) on acute myocardial infarction (AMI). Methods Mouse EPCs were isolated, purified, and identified by flow cytometry and uptake of DiI-ac-LDL. The target gene of miR-326-5p was predicted using target prediction algorithms and verified by dual-luciferase reporter assay, RT-qPCR, and Western blot. After EPCs were transfected with the agomir or antagomir of miR-326-5p, tube formation assay and Matrigel plug angiogenesis assay were conducted in four groups (NC, miR-326-5p agomir, miR-326-5p antagomir, and miR-326-5p agomir+Wnt1 agonist). In addition, a mouse model of MI was established and treated with the injection of miR-326-5p-EPCs, miR-326-5p-EPCs+ Wnt1 agonist, EPCs-NC, or PBS/control into the peri-infarcted myocardium. Subsequently, cardiac function was monitored by echocardiography at 7 and 28 days postoperatively. Finally, the infarcted hearts were collected at 28 days, and the size of myocardial infarction was measured by Masson’s trichrome staining and the neovascularization in the peri-infarcted area was examined through immunofluorescence staining. Results Luciferase reporter assay indicated that Wnt1 was a direct target of miR-326-5p. Using RT-qPCR and Western blot analysis, we further demonstrated that the expression level of Wnt1 was negatively correlated with miR-326-5p expression in EPCs. Both in vitro study of tube formation assay and in vivo investigation of subcutaneous Matrigel plug assay revealed that the miR-326-5p agomir could significantly enhance the angiogenic capacity of EPCs, and this effect was partially inhibited by Wnt1 agonist. Meanwhile, miR-326-5p antagomir could obviously reduce the the angiogenic capacity of EPCs in vivo compared with that in the NC group. Moreover, the transplantation of miR-326-5p-overexpressing EPCs in the ischemic hearts of mice significantly enhanced the angiogenesis in the peri-infarcted zone and improved the cardiac function. However, the enhanced capacity of angiogenesis of miR-326-5p-overexpressing EPCs was remarkably neutralized by Wnt1 agonist, accompanied by the decreased improvement in cardiac function. Conclusion miR-326-5p significantly enhanced the angiogenic capacity of EPCs. Transplantation of miR-326-5p-overexpressing EPCs improved cardiac function for AMI therapy, which can be a novel strategy for enhancing therapeutic angiogenesis in ischemic heart diseases.

scholarly journals Improvement of Cardiac Function in Mouse Myocardial Infarction after Transplantation of Epigenetically-Modified Bone Marrow Progenitor Cells

PLoS ONE ◽  
2011 ◽  
Vol 6 (7) ◽  
pp. e22550 ◽  
Author(s):  
Johnson Rajasingh ◽  
Jayakumar Thangavel ◽  
Mohammad R. Siddiqui ◽  
Ignatius Gomes ◽  
Xiao-pei Gao ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Bone Marrow ◽  
Cardiac Function ◽  
Progenitor Cells ◽  
Bone Marrow Progenitor Cells ◽  
Bone Marrow Progenitor

Implantation of cardiac progenitor cells using self-assembling peptide improves cardiac function after myocardial infarction

2010 ◽  
Vol 49 (6) ◽  
pp. 972-983 ◽  
Author(s):  
Masakuni Tokunaga ◽  
Mei-Lan Liu ◽  
Toshio Nagai ◽  
Koji Iwanaga ◽  
Katsuhisa Matsuura ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Cardiac Function ◽  
Progenitor Cells ◽  
Cardiac Progenitor Cells ◽  
Self Assembling
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