scholarly journals Priming seeds-method for increasing the germination of soybean seeds under drought stress conditions

2020 ◽  
Vol 25 (50) ◽  
pp. 105-111
Author(s):  
Zlatica Miladinov ◽  
Ivana Maksimović ◽  
Svetlana Balešević-Tubić ◽  
Vojin Đukić ◽  
Zorica Nikolić ◽  
...  
Keyword(s):  
Drought Stress ◽  
Seed Germination ◽  
Water Potential ◽  
Negative Impact ◽  
Cultivated Plants ◽  
Soybean Seeds ◽  
Sensitive Stage ◽  
Negative Effect ◽  
Improve Seed Germination ◽  
Successful Production

Drought is one of the most important factors limiting the successful production of cultivated plants. One of the most sensitive stage to the water deficit in plants is seed germination. There are various methods of pre-sowing treatments that aim to reduce the negative impact of drought stress and improve seed germination. One of them is priming seeds. The aim of this experiment was to examine the effect of priming seeds in solutions - KNO3 (1%), ascorbic acid - AsA (100 mgl-1) and potassium chloride KCl (1%) on the reduction of the negative effect of drought stress. The effect of drought stress was simulated using different concentrations of PEG 6000 (0 (control), -0.30, -0.51, -0.80 MPa). The results of the research showed that with the increase of water deficiency, the effect of priming seeds is greater. At the water potential of the solution of -0.30 MPa, germination energy and seed germination increased on average by 6.77% and 5.08%, while at the water potential of the solution of -0.80 MPa, the increase was 19.28% and 16.75%, respectively. Also, priming seeds significantly reduced the intensity of lipid peroxidation and the content of free proline. From all the above, it can be concluded that priming of seeds is a method that can serve to improve the germination of soybean seeds in conditions of drought stress.

scholarly journals The effect of biopreparations of nodules bacteria with carmoisine on the formation and functioning of the soybean – Bradyrhizobium japonicum symbiotic systems

2021 ◽  
Vol 17 (2) ◽  
pp. 123-129
Author(s):  
Ekaterina Kukol ◽  
Nadiya Vorobey ◽  
Petro Pukhtaievych ◽  
Sergii Kots
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Negative Impact ◽  
Root Nodules ◽  
Soybean Seeds ◽  
Biological Products ◽  
Dye Analysis ◽  
Full Flowering ◽  
Negative Effect ◽  
Glycine Max L ◽  
Beginning Of Flowering

Purpose. Investigate the formation and functioning of symbiotic systems of soybeans with nodule bacteria by ino­culation of seeds with biological products based on fungicide-resistant strains of Bradyrhizobium japonicum PC07 and B78 with different rates of synthetic carmoisine colorant. Methods. Physiological, microbiological, gas chromatography, statistical. Results. It was found that as a result of inoculation of soybean [Glycine max (L.) Merr] variety ‘Almaz’ with microbial preparations based on B. japonicum PC07 and B78, with the addition of carmoisine (0.25 and 0.5 g per 200 g of the preparation), the amount and the weight of nodules formed on the roots during the growing season were at the level of the control plants or exceeded them. The greatest difference in indicators of quantity and weight of root nodules between plants of control and experimental variants is noted in a phase of full flowering at inoculation by both strains of rhizobia and addition to biological products of various norms of dye. Analysis of nitrogen-fixing activity (NFA) of the formed symbiotic systems showed the absence of a negative effect of the synthetic colorant on its level. When inoculated with soybean seeds B. japonicum PC07 in the phase of three true leaves, NFA was higher by 15.6–25.9% and in the budding-beginning of flowering stage by 7.4–29.5% compared with control plants with the addition of 0.25 and 0.5 g of carmoisine, respectively. Against the background of bacterization of soybean seeds by strain B78 before the phase of full flowering of plants the level of N2 assimilation by adding 0.25 g of carmoisine to the vermiculite preparation was at the level of the control plants. During the period of full flowering, this figure exceeded the indicators of control plants by 7.6 and 18.8% with the introduction of 0.25 and 0.5 g of the colorant. Conclusions. Carmoisine can be applied in the further study of the effectiveness of its use as a dye identifier for controlling the uniformity of marking of loose bacterial preparations on seeds by adding 0.25 and 0.5 g per 200 g of a biopreparation, since this did not show a negative impact on the formation and functioning of the soybean – Bradyrhizobium japonicum symbiotic systems.

scholarly journals Stressful times in a climate crisis: how will aphids respond to more frequent drought?

2020 ◽  
Author(s):  
Daniel Joseph Leybourne ◽  
Katharine F Preedy ◽  
Tracy A Valentine ◽  
Jorunn IB Bos ◽  
Alison J Karley
Keyword(s):  
Drought Stress ◽  
Negative Impact ◽  
Meta Analysis ◽  
Chemical Defence ◽  
Plant Vigour ◽  
Quantitative Synthesis ◽  
Negative Effect ◽  
Species Combination ◽  
Underlying Mechanisms ◽  
Climate Crisis

AbstractAimAphids are abundant in natural and managed vegetation, supporting a diverse community of organisms and causing damage to agricultural crops. Using a meta-analysis approach, we aimed to advance understanding of how increased drought incidence will affect this ecologically and economically important insect group, and to characterise the underlying mechanisms.LocationGlobal.Time period1958–2020.Major taxa studiedAphids.MethodsWe used qualitative and quantitative synthesis techniques to determine whether drought stress has a negative, positive, or null effect on aphid fitness. We examined these effects in relation to 1) aphid biology, 2) the aphid-plant. species combination. We compiled two datasets: 1) a “global” dataset (n = 55 from 55 published studies) comprising one pooled effect size per study, and 2) an “expanded” dataset (n = 93) containing multiple datapoints per study, separated into different measures of aphid fitness but pooled across aphid-plant combinations. Where reported, we extracted data on the effect of drought on plant vigour, and plant tissue concentrations of nutrients and defensive compounds, to capture the potential causes of aphid responses.ResultsAcross all studies (“global” dataset), drought stress had a negative effect on aphid fitness: Hedges’ g = −0.57; 95% confidence interval (CI95) = ±0.31. The “expanded” dataset indicated that, on average, drought stress reduced aphid fecundity (g = − 0.98; CI95 = ±0.50) and increased development time (g = 1.13; CI95 = ±1.02). Furthermore, drought stress had a negative impact on plant vigour (g = −7.06; CI95 = ±2.86) and increased plant concentrations of defensive chemicals (g = 3.14; CI95 = ±3.14).Main conclusionsAphid fitness is typically reduced under drought, associated with reduced plant vigour and increased chemical defence in drought-stressed plants. We propose a conceptual model to predict drought effects on aphid fitness in relation to plant vigour and defence.

PD-L1 and survival in oral cavity squamous cell carcinoma

2020 ◽  
Vol 25 (4) ◽  
pp. 287-294
Author(s):  
S. I. Kutukova ◽  
N. P. Beliak ◽  
G. A. Raskin ◽  
M. S. Mukhina ◽  
Yu. V. Ivaskova ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Cavity ◽  
Cell Carcinoma ◽  
Tumor Cells ◽  
Squamous Cell ◽  
Immune Cells ◽  
Negative Impact ◽  
Progression Free Survival ◽  
Negative Effect ◽  
Effect Of Pd

Relevance. Prognostic value of PD-L1 expression in oral cavity squamous cell carcinoma (OCSCC) and its effect on survival is still controversial. It should be to determine the prognostic role of PD-L1 expression on tumor and immune cells of OCSCC and assess their effect on overall survival (OS) and progression-free survival (PFS).Materials and methods. A prospective study included 145 patients, first diagnosed with OCSCC. PD-L1 expression on tumor and immune cells, infiltrating tumor and its microenvironment, was assessed in all tumor samples by IHC, CPS was calculated. Cut-off values were determined by ROC analysis for identification of PD-L1 expression effect on OS and PFS.Results. Most patients with oral mucosa squamous cell carcinoma showed positive expression of PD-L1 on tumor (77.2%) and immune cells (92.4%). The median PD-L1 expression on tumor cells was 13.5% [1.0-40.0], the median PD-L1 expression on immune cells was 5.0% [1.0-11.0], and the median CPS – 18.0 [3.0-7.8]. Univariate and multivariate analyses revealed a significant negative effect of PD-L1 expression on immune cells ≤ 7% on OS (HR 0.66; 95% CI 0.45-0.93; p = 0.0498); PD-L1 expression in tumor cells ≤ 15% (HR 0.65; 95% CI 0.43-0.98; p = 0.0416) and CPS ≤ 21 (HR 0.62; 95% CI 0.44-0.92; p = 0.0183) for PFS. PD-L1 expression in tumor cells ≤ 6% (HR 0.71; 95% CI 0.47-1.08; p = 0.1096) and CPS ≤ 7 (RR 0.67; 95% CI 0.44-1.01; p = 0.0575) had a confident tendency to negative impact on OS.Conclusion. Positive PD-L1 expression in tumor and immune cells as well as CPS are effective additional factors in the prognosis of the disease course, OS and PFS in patients with OCSCC.

scholarly journals Relationships Among the Feeding Behaviors of a Mirid Bug on Cotton Leaves of Different Ages and Plant Biochemical Substances

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Haiyan Song ◽  
Zhaoke Dong ◽  
Lili Li ◽  
Zengbin Lu ◽  
Chao Li ◽  
...  
Keyword(s):  
Negative Impact ◽  
Leaf Age ◽  
Leaf Traits ◽  
Cotton Leaf ◽  
Young Plant ◽  
Feeding Behaviors ◽  
Probing Behavior ◽  
Negative Effect ◽  
Mirid Bug ◽  
The Relationship

Abstract Apolygus lucorum (Meyer-Dür) (Hemiptera: Miridae) tends to feed on young plant tissues. To explore the relationship between stylet probing behaviors of adult A. lucorum and conditions of cotton leaves, we conducted an experiment using electropenetrography (EPG). Behaviors were recorded on four cotton varieties, in relation to thickness and biochemical traits of differently-aged leaves. Cotton leaf age had a significant effect on the probing behavior of A. lucorum but cotton variety did not. One-day-old leaves of A. lucorum received the highest mean number of stylet probes (penetrations) per insect, and longest mean durations per insect of combined stylet probing or its components, cell rupture and ingestion behaviors. All of the leaf traits (thickness and biochemical substances) were similar among these four cotton varieties. Leaf thickness had a significantly negative effect on the same four variables above. Gossypol and tannin also had a negative impact on combined probing duration. Redundancy analysis showed that the four EPG variables were closely related to nutrient substances (amino acids, sugar, and water) while they had the opposite relationship with plant defense substances (gossypol and tannin). On cotton in the seedling stages, A. lucorum fed more readily on the youngest, thinnest leaves in our no-choice EPG experiments. Nutrients and chemical resistance substances determined the probing duration of A. lucorum. Our findings can contribute to better understanding of patterns of feeding and host consumption by A. lucorum, ultimately improving cotton resistance to A. lucorum.

Does N deposition mitigate the adverse impacts of drought stress on plant seed germination and seedling growth?

2020 ◽  
Vol 109 ◽  
pp. 103650
Author(s):  
Shu Wang ◽  
Mei Wei ◽  
Bingde Wu ◽  
Huiyuan Cheng ◽  
Kun Jiang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Seed Germination ◽  
Seedling Growth ◽  
N Deposition ◽  
Plant Seed ◽  
Adverse Impacts

scholarly journals Analysis of User Needs on Downloading Behavior of English Vocabulary APPs Based on Data Mining for Online Comments

Mathematics ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 1341
Author(s):  
Tinggui Chen ◽  
Lijuan Peng ◽  
Jianjun Yang ◽  
Guodong Cong
Keyword(s):  
Negative Impact ◽  
Positive Impact ◽  
User Needs ◽  
User Requirements ◽  
Functional Requirements ◽  
User Comments ◽  
Online Comments ◽  
Significant Negative Impact ◽  
Negative Effect ◽  
New Type

With highly developed social media, English learning Applications have become a new type of mobile learning resources, and online comments posted by users after using them have not only become an important source of intellectual competition for enterprises, but can also help understand customers’ requirements, thereby improving product functionalities and service quality, and solve the pain points of product iteration and innovation. Based on this, this paper crawled the online user comments of three typical APPs (BaiCiZhan, MoMoBeiDanCi and BuBeiDanCi), through emotion analysis and hotspot mining technology, to obtain user requirements and then the K-means clustering method was used to analyze user requirements. Finally, quantile regression is used to find out which user needs have an impact on the downloads of English vocabulary APPs. The results show that: (1) Positive comments have a more significant impact on users’ downloads behavior than negative online comments. (2) English vocabulary APPs with higher downloads, both the 5-star user ratings and the increase of emotional requirement have a negative effect on the increase in APP downloads, while the enterprise’s service requirement improvement has a positive effect on the increase of APP downloads. (3) Regarding English vocabulary APPs with average or high downloads, improving the adaptability and Appearance requirements have significant negative impact on downloads. (4) The functional requirements to improve products will have a significant positive impact on the increase in downloads of English vocabulary APPs.

Seed germination of pitaya (Hylocereus spp.) as affected by seed extraction method, storage, germination conditions, germination assessment approach and water potential

2019 ◽  
Vol 33 (3) ◽  
pp. 372-394
Author(s):  
Dessireé Zerpa-Catanho ◽  
Andrés Hernández-Pridybailo ◽  
Viviana Madrigal-Ortiz ◽  
Adonay Zúñiga-Centeno ◽  
Carolina Porras-Martínez ◽  
...  
Keyword(s):  
Seed Germination ◽  
Water Potential ◽  
Extraction Method ◽  
Assessment Approach ◽  
Seed Extraction
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