Toxic effects of heavy metals (cadmium plus mercury) on haematological parameters and DNA damage in Lates calcarifer

Perfluorononanoic acid (PFNA), a member of PFAS, is frequently detected in human blood and tissues, even in follicular fluid of women. The exposure of PFNA, but not PFOA and PFOS, is positively correlated with miscarriage and increased time to pregnancy. Toxicological studies indicated that PFNA exposure is associated with immunotoxicity, hepatotoxicity, developmental toxicity, and reproductive toxicity in animals. However, there is little information regarding the toxic effects of PFNA on oocyte maturation. In this study, we investigated the toxic effects of PFNA exposure on mouse oocyte maturation in vitro. Our results showed that 600 μM PFNA significantly inhibited germinal vesicle breakdown (GVBD) and polar body extrusion (PBE) in mouse oocytes. Our further study revealed that PFNA induced abnormal metaphase I (MI) spindle assembly, evidenced by malformed spindles and mislocalization of p-ERK1/2 in PFNA-treated oocytes. We also found that PFNA induced abnormal mitochondrial distribution and increased mitochondrial membrane potential. Consequently, PFNA increased reactive oxygen species (ROS) levels, leading to oxidative stress, DNA damage, and eventually early-stage apoptosis in oocytes. In addition, after 14 h culture, PFNA disrupted the formation of metaphase II (MII) spindle in most PFNA-treated oocytes with polar bodies. Collectively, our results indicate that PFNA interferes with oocyte maturation in vitro via disrupting spindle assembly, damaging mitochondrial functions, and inducing oxidative stress, DNA damage, and early-stage apoptosis.

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Effects of heavy metals Cd2+, Pb2+ and Zn2+ on DNA damage of loach Misgurnus anguillicaudatus

Frontiers of Biology in China ◽

10.1007/s11515-008-0012-3 ◽

2008 ◽

Vol 3 (1) ◽

pp. 50-54 ◽

Cited By ~ 16

Author(s):

Yingmei Zhang ◽

Yejing Wang ◽

Runliu Yu ◽

Sheng Zhang ◽

Zhenbin Wu

Keyword(s):

Heavy Metals ◽

Dna Damage ◽

Misgurnus Anguillicaudatus

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CHAPTER 27. Heavy Metals – Lead, Mercury and Cadmium and Their Impact on DNA Damage Measured by the Micronucleus Assay

Issues in Toxicology - The Micronucleus Assay in Toxicology ◽

10.1039/9781788013604-00435 ◽

2019 ◽

pp. 435-449

Author(s):

Armen Nersesyan ◽

Michael Kundi ◽

Miroslav Mišík ◽

Georg Wultsch ◽

Siegfried Knasmueller

Keyword(s):

Heavy Metals ◽

Dna Damage ◽

Micronucleus Assay

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Copper in sludge — are the Toxic Effects of Copper and other Heavy Metals Additive?

Copper in Animal Wastes and Sewage Sludge ◽

10.1007/978-94-009-8503-2_14 ◽

1981 ◽

pp. 204-222

Author(s):

P. Beckett

Keyword(s):

Heavy Metals ◽

Toxic Effects

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The Heavy Metals in Agrosystems and Impact on Health and Quality of Life

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2015.048 ◽

2015 ◽

Vol 3 (2) ◽

pp. 345-355 ◽

Cited By ~ 7

Author(s):

Adnan Tutic ◽

Srecko Novakovic ◽

Mitar Lutovac ◽

Rade Biocanin ◽

Sonja Ketin ◽

...

Keyword(s):

Heavy Metals ◽

Animal Health ◽

Toxic Effects ◽

Basic Principles ◽

Essential Trace Elements ◽

Ionic Bonds ◽

Negative Impacts ◽

Surface And Groundwater ◽

Quality Surface

The metal is a chemical element that conducts electricity well and heat, and the nonferrous metals builds cations and ionic bonds. Heavy metals include metals whose density is higher than 5 g/cm3. The whole range of the metal is in the form of essential trace elements, essential for a number of functions in the human body, and its deficiency results in a lack of occurrence of a serious symptom. The best examples are anemia lack of iron, lack of chromium in diabetes, growth problems in lack of nickel. Other elements such as lead, cadmium, mercury, arsenic and molybdenum have been shown to exhibit large quantities of toxic effects. The paper examines the problem of heavy metals originating from agriculture on agroecosystems. This group of pollutants is considered the most important cause of degradation of soil quality, surface and groundwater and direct causal adverse effects on human and animal health. In order to complete the environmental monitoring of pollutants, these main categories, origins, and possible negative impacts of the basic principles of preventing their toxic effects were examined.

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Analisis Kandungan Logam Berat Pada Ikan Kakap Putih (Lates calcarifer Bloch) di Perairan Mimika Papua

Jurnal Ilmu Lingkungan ◽

10.14710/jil.17.2.256-263 ◽

2019 ◽

Vol 17 (2) ◽

pp. 256

Author(s):

Rosye H.R. Tanjung ◽

Suwito Suwito ◽

Vita Purnamasari ◽

Suharno Suharno

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Metal Content ◽

Heavy Metal Contamination ◽

Sea Water ◽

Good Condition ◽

Quality Standard ◽

Heavy Metal Content ◽

Lates Calcarifer ◽

Tailings Sand

Kebutuhan bahan pangan sangat tergantung pada ketersediaanya di lingungan. Bahan pangan yang diperlukan untuk memenuhi kebutuhan sehari-hari harus sehat dan bebas dari bahan pencemar, termasuk logam berat. Ikan kakap putih (Lates calcarifer) sering dijumpai pada kawasan muara sungai di hampir seluruh wilayah Indonesia, bahkan di Papua. Tujuan penelitian ini adalah untuk mengkaji kandungan logam berat Pb, Cd, Cu, Fe, As, dan Hg pada ikan kakap putih (L. calcarifer) yang hidup di perairan estuari Mimika Papua. Perairan estuari di Mimika diketahui sebagai salah satu daerah pengendapan pasir sisa tambang (tailing). Metode yang digunakan adalah survei dan analisis laboratorium kandungan logam berat pada tubuh ikan. Analisis Pb, Cd, Cu, Fe, As, dan Hg ditentukan dengan spektroskopi serapan atom (AAS, Atomic Absorpsion Spectroscopy). Penentuan tingkat pencemaran logam berat dilakukan dengan Metode Standar APHA 3113 Cetac Technologies SPR IDA. Analisis data dilakukan dengan membandingkan kandungan logam berat dalam air dengan baku mutu air laut menurut SK MNLH No. 51 tahun 2004. Untuk kandungan logam berat pada organ tubuh ikan dibandingkan dengan kandungan maksimum logam berat berdasarkan SNI 7387: 2009 tentang batas maksimum cemaran logam berat bahan pangan. Kandungan logam berat pada ikan kakap putih masih tergolong aman dikonsumsi karena mengandung logam berat di bawah ambang batas baku mutu. Kondisi ini didukung oleh hasil analisis logam berat pada air yang menunjukkan masih dalam kondisi baik. Kata kunci: L. calcarifer, logam berat, Sungai Kamora, Sungai Ajkwa, Mimika. The need for food depends on the availability in the environment. Foods needed to meet daily needs should be healthy and free of pollutants, including heavy metals. White snapper (Lates calcarifer) is often found in the estuary of the river in almost all parts of Indonesia, even in Papua. The purpose of this research is to study the heavy metal content of Pb, Cd, Cu, Fe, As, and Hg on white snapper (L. calcarifer) which live in Mimika Papua estuary waters. The estuary waters of Mimika are known as one of the deposition areas of tailings sand. The method used is survey and laboratory analysis of heavy metal content in fish body. Analysis of Pb, Cd, Cu, Fe, and Hg was determined by Atomic Absorption Spectroscopy (AAS). Determination of the level of heavy metal contamination was done by Standard Method of APHA 3113 Cetac Technologies SPR IDA. Data analysis was done by comparing the heavy metal content in water with sea water quality standard according to SK MNLH No. 51 year 2004. For heavy metal content in fish body organs compared with maximum content of heavy metals based on SNI 7387: 2009 on the maximum limit of heavy metal food contamination. The content of heavy metals in white snapper is still considered safe for consumption because its below the quality standard threshold. This condition is supported by the results of heavy metal analysis on the water which shows still in good condition. Key words: L. calcarifer, heavy metal, Kamora River, Ajkwa River, Mimika.

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