scholarly journals Biochemical analysis of Origanum vulgare seeds by fourier-transform infrared (FT-IR) spectroscopy and gas chromatography-mass spectrometry (GC-MS)

2015 ◽  
Vol 7 (9) ◽  
pp. 221-237 ◽  
Author(s):  
Jasim Al Tameme Huda ◽  
Hadi Hameed Imad ◽  
Ali Idan Salah ◽  
Yahya Hadi Mohammed
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Fourier Transform ◽  
Ir Spectroscopy ◽  
Biochemical Analysis ◽  
Fourier Transform Infrared ◽  
Gas Chromatography Mass Spectrometry ◽  
Origanum Vulgare ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

Attenuated Total Reflection Fourier Transform Infrared (ATR FT-IR) for Rapid Determination of Microbial Cell Lipid Content: Correlation with Gas Chromatography-Mass Spectrometry (GC-MS)

2017 ◽  
Vol 71 (10) ◽  
pp. 2344-2352 ◽  
Author(s):  
Aaron Millan-Oropeza ◽  
Rolando Rebois ◽  
Michelle David ◽  
Fathi Moussa ◽  
Alexandre Dazzi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Fourier Transform ◽  
Lipid Content ◽  
Fourier Transform Infrared ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Transmission Mode ◽  
Gas Chromatography Mass Spectrometry ◽  
Ft Ir

There is a growing interest worldwide for the production of renewable oil without mobilizing agriculture lands; fast and reliable methods are needed to identify highly oleaginous microorganisms of potential industrial interest. The aim of this study was to demonstrate the relevance of attenuated total reflection (ATR) spectroscopy to achieve this goal. To do so, the total lipid content of lyophilized samples of five Streptomyces strains with varying lipid content was assessed with two classical quantitative but time-consuming methods, gas chromatography–mass spectrometry (GC-MS) and ATR Fourier transform infrared (ATR FT-IR) spectroscopy in transmission mode with KBr pellets and the fast ATR method, often questioned for its lack of reliability. A linear correlation between these three methods was demonstrated allowing the establishment of equations to convert ATR values expressed as CO/amide I ratio, into micrograms of lipid per milligram of biomass. The ATR method proved to be as reliable and quantitative as the classical GC-MS and FT-IR in transmission mode methods but faster and more reproducible than the latter since it involves far less manipulation for sample preparation than the two others. Attenuated total reflection could be regarded as an efficient fast screening method to identify natural or genetically modified oleaginous microorganisms by the scientific community working in the field of bio-lipids.

scholarly journals Extract of Albizia kalkora leaves

2020 ◽  
Vol 24 (3 Part A) ◽  
pp. 1721-1728
Author(s):  
Kun Hou ◽  
Dongfang Zheng ◽  
Yong Zhao ◽  
Ting Wang ◽  
Yafeng Yang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Fourier Transform ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Components ◽  
Chromatography Mass Spectrometry ◽  
Medicinal Value ◽  
Potential Applications ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

In this paper, a mixture of methanol, ethanol, benzene and ethanol were used to extract the leaves of Albizia kalkora (Roxb.) Prain. Then, using Fourier transform infrared (FT-IR) spectroscopy, gas chromatography-mass spectrometry (GC-MS), pyrolysis as chromatography - mass spectrometry, and thermogravimetric analysis (TGA) and other advanced instruments Albizia kalkoraleaves were analyzed to determine their composition. According to the test data, Albizia kalkoraleaves contain epinephrine, trans squalene, stachydrine and other chemical components with high medicinal value, indicating that Albizia kalkoraleaves have potential applications in the pharmaceutical industry. This study has expanded the utilization value of Albizia kalkoratrees and provided technical support for the resource utilization of Albizia kalkoratrees.

scholarly journals Bacterial Typing and Identification Based on Fourier Transform Infrared Spectroscopy

2020 ◽  
Author(s):  
Huayan Yang ◽  
Fangling Wu ◽  
Fuxin Xu ◽  
Keqi Tang ◽  
Chuanfan Ding ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Ir Spectroscopy ◽  
Bacterial Culture ◽  
Clinical Laboratory ◽  
Fourier Transform Infrared ◽  
Identification Accuracy ◽  
Bacterial Typing ◽  
Typical Experiment ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

Abstract Fourier transform infrared (FT-IR) spectroscopy is a label-free and highly sensitive technique that provides complete information on the chemical composition of biological samples. The bacterial FT-IR signals are extremely specific and highly reproducible fingerprint-like patterns, making FT-IR an efficient tool for bacterial typing and identification. Due to the low cost and high flux, FT-IR has been widely used in hospital hygiene management for infection control, epidemiological studies, and routine bacterial determination of clinical laboratory values. However, the typing and identification accuracy could be affected by many factors, and the bacterial FT-IR data from different laboratories are usually not comparable. A standard protocol is required to improve the accuracy of FT-IR-based typing and identification. Here, we detail the principles and procedures of bacterial typing and identification based on FT-IR spectroscopy, including bacterial culture, sample preparation, instrument operation, spectra collection, spectra preprocessing, and mathematical data analysis. Without bacterial culture, a typical experiment generally takes <2 h.

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