scholarly journals Preliminary study of physiopathological changes associated with the ethanolic extract of the stem leaves of Phyllanthus amarus in rats

2018 ◽  
Vol 9 (1) ◽  
pp. 1-8
Author(s):  
DANDA Amina MAHAMADOU ◽  
Sèlidji Eugène ATTAKPA ◽  
Gbèssohélè Justin BÉHANZIN ◽  
Abdou Madjid AMOUSSA ◽  
Latifou LAGNIKA ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Phyllanthus Amarus ◽  
Preliminary Study

scholarly journals In Vitro and In Vivo Antimalarial Activities and Toxicological Assessment of Pogostemon Cablin (Blanco) Benth

2020 ◽  
Vol 25 ◽  
pp. 2515690X2097838
Author(s):  
Arisara Phuwajaroanpong ◽  
Prapaporn Chaniad ◽  
Natharinee Horata ◽  
Saowanee Muangchanburee ◽  
Kantarakorn Kaewdana ◽  
...  
Keyword(s):  
Body Weight ◽  
Antimalarial Activity ◽  
Ethanolic Extract ◽  
Vero Cells ◽  
Toxicological Assessment ◽  
Pogostemon Cablin ◽  
Dehydrogenase Enzyme ◽  
Preliminary Study

The aim of this study was to investigate the antimalarial activities and toxicity of Pogostemon cablin extracts. In vitro activities against the chloroquine-resistant Plasmodium falciparum K1 strain were assessed by using the Plasmodium lactate dehydrogenase enzyme (pLDH) assay, while in vivo activity against the Plasmodium berghei ANKA strain in mice was investigated using a 4-day suppressive test. The in vitro and in vivo toxicity were determined in Vero cells and mice, respectively. The ethanolic extract possessed antimalarial activity with an IC50 of 24.49 ± 0.01 µg/ml, whereas the aqueous extract showed an IC50 of 549.30 ± 0.07 µg/ml. Cytotoxic analyses of the ethanolic and aqueous extracts revealed a nontoxic effect on Vero cells at a concentration of 80 µg/ml. Based on a preliminary study of in vitro antimalarial activity, the ethanolic extract was chosen as a potential agent for further in vivo antimalarial activity analysis in mice. The ethanolic extract, which showed no toxic effect on mice at a dose of 2000 mg/kg body weight, significantly suppressed parasitemia in mice by 38.41%, 45.12% and 89.00% at doses of 200, 400 and 600 mg/kg body weight, respectively. In conclusion, this study shows that the ethanolic P. cablin extract possesses in vitro and in vivo antimalarial activity without toxic effects.

scholarly journals Toxicological Characterization of Six Plants of the Beninese Pharmacopoeia Used in the Treatment of Salmonellosis

2019 ◽  
Vol 2019 ◽  
pp. 1-12
Author(s):  
Boris Legba ◽  
Victorien Dougnon ◽  
Esther Deguenon ◽  
Jerrold Agbankpe ◽  
Maximin Senou ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Ethanolic Extract ◽  
Optical Emission ◽  
Chemical Characteristic ◽  
Psidium Guajava ◽  
Phyllanthus Amarus ◽  
Traditional Treatment ◽  
Variable Threshold ◽  
Traditional Medicines ◽  
Ethanolic Extracts

Recent studies reported interesting ethnopharmacological, antibacterial, and phytochemical data on some medicinal plants used in the traditional treatment of salmonellosis in Benin. Unfortunately, very little data exists on the toxicity of these species. This study aims to evaluate chemical characteristic of six Benin pharmacopoeial plants used in the traditional treatment of salmonellosis in Benin. The acute toxicity of aqueous and ethanolic extracts ofPsidium guajava,Vernonia amygdalina,Cajanus cajan,Phyllanthus amarus,Uvaria chamae, andLantana camarawas evaluated according to OECD Guideline 423 at a single dose of 2000 mg/kg body weight on Wistar rats. Histological sections were performed on the liver and kidneys to confirm hematological and biochemical data. The content of aluminum, chromium, cadmium, copper, iron, lead, zinc, arsenic, selenium, and manganese was measured in 10 mg of each extract by the inductively coupled plasma optical emission spectroscopy (ICPOES) method. The results of our study generally show the absence of significant effect of the extracts on the hematological and biochemical parameters of the rats. However, with the exception of the aqueous and ethanolic extracts ofPsidium guajavaroot and the ethanolic extract ofPhyllanthus amarus(P>0.05), all the extracts have a significant effect on the aspartate aminotransferase (ASAT) level, with a variable threshold of significance (0.0001< P ≤ 0.05). No mortalities and no renal histological conditions were recorded in the treated rats. In general, the heavy metal contents of the extracts do not exceed the standards set by the WHO/FDA except for a few extracts. Arsenic was not detected in any extract, while aluminum and chromium were detected at levels above the WHO/FDA standards. On the basis of these data, it appears that the six plants studied do not show any toxicity. In view of the pharmacological and chemical data previously available, these plants are good candidates for the development of improved traditional medicines with antibacterial and particularly anti-Salmonellaproperties.

IN-VIVO IMMUNOMODULATORY ACTIVITY OF ETHANOLIC EXTRACTS OF AERIAL PARTS OF PHYLLANTHUS AMARUS PLANT IN CYCLOPHOSPHAMIDE INDUCED MICE

2021 ◽  
Vol 10 (1) ◽  
pp. 11-15
Author(s):  
Veershetty Hachhe ◽  
Siddaiah M
Keyword(s):  
Ethanolic Extract ◽  
Treated Group ◽  
Immunomodulatory Activity ◽  
Phyllanthus Amarus ◽  
Group Iii ◽  
Platelet Counts ◽  
Aerial Parts ◽  
Ethanolic Extracts ◽  
Respective Treatment

Objective: To evaluate the immunomodulatory activity of ethanolic extract of aerial parts of phyllanthus amarus plant in cyclophosphamide induced mice. Materials and Method: To evaluate the immunomodulatory activity of ethanolic extracts of Aerial parts of phyllanthus amarus plant. on cyclophosphamide induced immunosuppression in mice. ethanolic extracts of aerial parts of phyllanthus amarus plant,  (500mg/kg p.o.) were administered 13 days to albino mice and cyclophosphamide (30mg/kg i.p.) was administered on 11th,12th and 13th days 1 hour after the administration of the respective treatment. On the 14th day blood was collected by retro orbital puncture and the activity was evaluated by determining the RBC, Hb%, Platelet, total WBC and differential counts. Ethanolic extracts of aerial parts phyllantus amarus plant. showed very significant (p<0.001) counteracting effect to cyclophosphamide induced reduction in total WBC, DLC and platelet counts & significant (P<0.01) effect to that of reduction in RBC counts and Hb %. The significant inmmunostimulant effect of the ethanolic extracts of aerial parts of phyllanthus amarus plant on cyclophosphamide induced myelosuppression may be attributed towards the collective presence of saponins, sterols and tannins in the extracts, which suggest the immunomodulatory activities of the ethanolic extracts of phyllanthusamarus plant, leaves,stem and fruits. Results: The results of the study revealed the dose dependent effect of theextracts in counteracting the myelosuppression induced by cyclophosphamide as inducated by increase in RBC total WBC platelet counts, Hb% and DLC in the extract treated group (III &IV), when compared to cyclophoshamide treated group (Group II). Conclusion: Ethanolic extract of phyllanthus amarus would contribute for significant immunostimulating activity of In-Vivo Keywords: Phyllanthus amarus, Cyclophosphamide, Immunomodulatory, Myelosuppression

scholarly journals Preclinical screening of phyllanthus amarus ethanolic extract for its analgesic and antimicrobial activity

2015 ◽  
Vol 7 (4) ◽  
pp. 378 ◽  
Author(s):  
Shyamjith Manikkoth ◽  
SSham Bhat ◽  
KSundeep Hegde ◽  
Sharath Chandrashekhar ◽  
SN Rao
Keyword(s):  
Antimicrobial Activity ◽  
Ethanolic Extract ◽  
Phyllanthus Amarus ◽  
Analgesic And Antimicrobial Activity ◽  
Preclinical Screening

scholarly journals Therapeutic Effects of Aqueous and Ethanolic Extracts of Phyllanthus amarus on 1, 2 Dimethylhydrazine Induced Colon Carcinogenesis in Balb/C Mice

2020 ◽  
pp. 36-43
Author(s):  
F. O. Omoregie ◽  
G. E. Eriyamremu ◽  
Suman Kapur
Keyword(s):  
Colon Cancer ◽  
Body Weight ◽  
Aqueous Extract ◽  
Plant Extracts ◽  
Ethanolic Extract ◽  
Therapeutic Effects ◽  
Dependent Manner ◽  
Phyllanthus Amarus ◽  
Traditional Use ◽  
Dose Dependent

Context: Phyllanthus amarus is traditionally used for various infections, inflammation and cancer. 1,2 Dimethylhydrazine is a potent colon cancer inducer in animals. Objective: The present study investigated the effects of aqueous and ethanolic extract of Phyllanthus amarus on 1, 2 Dimethylhydrazine induced colon cancer in BALB/c Mice. Materials and Methods: 30 female Balb/C Mice of weight 18-30 g were acclimatized for a week and randomized into 6 groups (5 per group). Group A (-DMH), Group B (+DMH), Group C (DMH+250 mg/kg body weight of ethanolic extract of P. amarus), Group D (DMH+350 mg/kg body weight of ethanolic of P. amarus), Group E (DMH + 250 mg/kg body weight of aqueous extract of P. amarus), Group F (DMH+ 350 mg/kg body weight of aqueous extract of P. amarus). 20 mg/kg body weight of DMH was administered orally for 21 days (twice a week). The plant extracts were administered daily for 3 weeks with the aid of a gavage immediately after colon cancer induction. Colon cancer was evaluated by the formation of Aberrant Cryptic Foci in the colon of DMH treated mice. Results: Administration of the plant extracts (aqueous and ethanolic) ameliorated the carcinogenic effect of DMH in the colon of DMH treated mice in a dose dependent manner by significantly reducing the number of Aberrant Cryptic Foci formed in extract treated mice by 38% for 350 mg/kg body of ethanolic extract and by 22% for 350 mg/kg body of aqueous extract of Phyllanthus amarus. Conclusion: The studied extracts had ameliorative potential on DMH induced colon cancer in Balb/C mice in a dose dependent manner providing evidence for the traditional use of this herb for treatment/prevention of cancer. Notably, 350 mg/kg body of both extracts showed better reduction of Aberrant Cryptic Foci compared to 250 mg/kg body of both ethanolic and aqueous extracts of Phyllanthus amarus.

scholarly journals Combination of Phyllanthus amarus Schum. & Thonn. and Gymnema sylvestre R. Br. for treatment of diabetes and its long-term complications

2021 ◽  
Vol 16 (3) ◽  
pp. 232-240
Author(s):  
T. M. Le ◽  
Ch. D.P. Nguyen ◽  
A. C. Ha
Keyword(s):  
Ethanolic Extract ◽  
Gymnema Sylvestre ◽  
Phyllanthus Amarus ◽  
Patients With Diabetes ◽  
Treatment Of Diabetes ◽  
Ethanolic Extraction ◽  
Anti Inflammatory ◽  
Patients Experience ◽  
Solid Liquid

Objectives. The amount of patients with diabetes is increasing, and such patients experience several long-term complications. Therefore, finding a method to treat the disease and its complications is an urgent issue worldwide. In Vietnam, Phyllanthus amarus Schum. & Thonn. (PA) and Gymnema sylvestre R. Br. (GS) are common herbs used in traditional therapy including diabetes treatment. This study aimed to combine PA and GS to extend their bioactivities in antidiabetes, antioxidant, and anti-inflammatory treatments.Methods. Here, PA and GS powders were mixed at different ratios for extraction. Ethanolic extract was used to detect bioactive compounds, bioactivities, and appropriate ratios of the mixtures.Results. The optimal ratio for the PA and GS combination was 2 : 1 (g/g). The ethanolic extraction of the 2 : 1 sample at 50°C over two hours with a solid/liquid ratio of 1 : 10 achieved a high yield of 14.37%. This sample exhibited good α-glucosidase inhibition activity with a half-maximal inhibitory concentration (IC50) of 9.74 µg/mL, antioxidant activity with an IC50 of 29.87 µg/mL, and anti-inflammatory activity with an IC15 of 400 µg/mL.Conclusions. The study confirmed that combining PA and GS can have high α-glucosidase inhibition as well as antioxidant and anti-inflammatory effects.

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