scholarly journals Embryonic development of anemone fishes in captivity

2017 ◽  
Vol 8 (1) ◽  
pp. 1-13 ◽  
Author(s):  
Uthaya Siva Muthuramalingam ◽  
Abdul Badhul Haq Mohideen
Keyword(s):  
Embryonic Development ◽  
In Captivity

Spawning induction and embryonic development of Salminus hilarii (Characiformes: Characidae)

Zygote ◽  
2020 ◽  
Vol 28 (5) ◽  
pp. 377-387
Author(s):  
Bruno Cavalheiro Araújo ◽  
Paulo Henrique Mello ◽  
Renata Guimarães Moreira ◽  
Alexandre Wagner Silva Hilsdorf ◽  
Victor Hugo Marques ◽  
...  
Keyword(s):  
Embryonic Development ◽  
River Basin ◽  
Fertilization Rate ◽  
Tropical Fish ◽  
Perivitelline Space ◽  
Artificial Reproduction ◽  
Yolk Mass ◽  
Tietê River ◽  
In Captivity ◽  
H 51

SummaryThe present study aimed to evaluate two different methods of artificial reproduction induction, and characterize the embryonic development of Salminus hilarii. Different than observed for other tropical fish species, artificial reproduction induction followed by hand-stripping of gametes was considered unfeasible for S. hilarii, as no gamete fertilization was observed. However, females that were induced and spawned naturally presented a fertilization rate of 65.64 ± 0.54%. With a mean temperature of 26.20 ± 0.90ºC it was possible to clearly distinguish a large perivitelline space at 14 min after fertilization (AF) and at 49 min AF more than 50% of the embryos presented two blastomeres, and these cleavages occurred until 1 h 54 min AF. The gastrula phase was characterized at 3 h 56 min AF, and blastopore closure was observed at 8 h 31 min AF. At 9 h 1 min, organogenesis started, with a clear distinction of the yolk mass, embryonic axis, cephalic and caudal regions; at 11 h 51 min AF the embryos already had advanced segmentation and a free tail. Total hatching occurred at 21 h 17 min and after opening the mouth, which occurred at 33 h 9 min, the larvae of S. hilarii presented a strong and characteristic cannibalism. This information can be considered fundamental to improving S. hilarii production in captivity and for collaboration with a conservation programme in the upper Tietê river basin.

scholarly journals Influence of spawning procedure on gametes fertilization success in Salminus hilarii Valenciennes, 1850 (Teleostei: Characidae): Implications for the conservation of this species

2011 ◽  
Vol 9 (2) ◽  
pp. 363-370 ◽  
Author(s):  
Renato M. Honji ◽  
Paulo H. Mello ◽  
Bruno C. Araújo ◽  
Jandyr A. Rodrigues-Filho ◽  
Alexandre W. S. Hilsdorf ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Morphological Difference ◽  
Fertilization Rate ◽  
Fertilization Success ◽  
Group 4 ◽  
In Captivity ◽  
In The Wild ◽  
Broodstock Management ◽  
Group 3 ◽  
Group 2

Artificial reproduction and gamete fertilization were evaluated in Salminus hilarii wild and domesticated broodstocks. Wild and domesticated broodstocks were artificially induced to reproduction using a carp pituitary treatment. Four groups were considered: Group 1 (G1), fish caught in the wild maintained for three years in the same conditions as the domesticated broodstocks and spawned naturally; Group 2 (G2), broodstock born and raised in captivity and spawned naturally; Group 3 (G3), wild broodstocks, which were manually stripped for gamete collection and dry fertilization; and Group 4 (G4), domesticated males and females, also manually stripped. Oocytes, eggs, and larvae were sampled at different time intervals throughout embryonic development. Yolk sac absorption occurred approximately 24-29 h after hatching. Twenty-six h after hatching, the larvae mouths opened. Cannibalism was identified just 28-30 h after hatching. There was no morphological difference in embryonic development among all groups. The number of released eggs per gram of female was: G1: 83.3 ± 24.5 and G2: 103.8 ± 37.4; however, the fertilization success was lower in G2 (42.0 ± 6.37 %) compared with G1 (54.7 ± 3.02%) (P = 0.011). Hand-stripping of oocytes was not successful and the fertilization rate was zero. The reproduction of this species in captivity is viable, but it is necessary to improve broodstock management to enhance fertilization rates and obtain better fingerling production for restocking programs.

scholarly journals Early development of the Peruvian rock seabass Paralabrax humeralis (Teleostei: Serranidae): morphological description of the embryonic and yolk-sac larval stages

2021 ◽  
Vol 49 (3) ◽  
pp. 520-525
Author(s):  
Masatoshi Futagawa ◽  
Jessica Pizarro ◽  
German Bueno ◽  
Juan Pablo Díaz
Keyword(s):  
Embryonic Development ◽  
Water Temperature ◽  
Early Development ◽  
Yolk Sac ◽  
Morphological Description ◽  
Cleavage Stage ◽  
Pigmentation Pattern ◽  
Caudal Region ◽  
Larval Stages ◽  
In Captivity

Describe the embryonic development of Paralabrax humeralis (Peruvian rock seabass) and the present morphology of its eggs and yolk-sac larvae using the eggs spawned by P. humeralis broodstock in captivity. The spawning occurred naturally and spontaneously in early November 2018. The egg is pelagic and round, with a diameter of 0.98 ± 0.02 mm, an oil globule, and a diameter of 0.2 ± 0.02 mm. Embryonic development started with meroblastic cleavage, reaching the first cleavage stage at 0.4 h post-fertilization (HPF), and reached 64 cells at 2.2 HPF. Blastula period, 128 cells to 30% epiboly, end at 11.3 HPF. Gastrula period, 50% epiboly to 90% epiboly, end at 19.6 HPF. In the organogenesis period, forming Kupffer’s vesicle appeared at 22.5 HPF, the separation of the caudal fin from the yolk at 30.3 HPF, and the hatching of the first larvae at 47.9 HPF. Water temperature was kept at 17.2 ± 0.2°C. The yolk-sac larvae measured 2.22 ± 0.1 mm with a pigmentation pattern of pinpoint melanophores, all along with the embryo and xanthophores in the cephalic region, trunk, and caudal region, as well as in the oil globule. The larva takes feeds from three days post-hatch-out.

scholarly journals Embryonic development and spawning pattern of Trisopterus luscus (Teleostei: Gadidae) under controlled conditions

2010 ◽  
Vol 91 (6) ◽  
pp. 1281-1287 ◽  
Author(s):  
Alexandre Alonso-Fernández ◽  
Alba Ruth Vergara ◽  
Fran Saborido-Rey
Keyword(s):  
Embryonic Development ◽  
Early Ontogeny ◽  
Batch Size ◽  
Marine Research ◽  
Spawning Frequency ◽  
Size Dependent ◽  
Controlled Conditions ◽  
In Captivity ◽  
Natural Photoperiod ◽  
Ría De Vigo

Knowledge on early ontogeny of Trisopterus luscus is scarce and incomplete. This paper describes the first successful attempt ever at achieving the natural spawning in captivity for this species. The reproductive specimens used for this study were obtained in the Ría de Vigo in September 2008 and subsequently transported to the facilities of the Institute of Marine Research (CSIC). The spawning frequency was variable among females, 1.8–4.6 days, but no size-dependent trend was determined. The average batch size, i.e. the number of eggs released per batch, has proven significantly dependent on female length and weight. In the present study the complete embryonic development in controlled conditions (sand–filtered seawater at ~13°C, with natural photoperiod), from egg to hatched larva, has been described for the first time ever. The development of pouting eggs was divided into six stages, based on the artificially-reared material. Pouting eggs are pelagic with a smooth, clear and spherical chorion, and a homogeneous yolk. The perivitelline space is narrow and oil globules are absent. Live eggs fertilized were 0.95–1.10 mm in diameter. The embryo hatches as a yolk-sac larva with closed mouth and gut. The pouting eggs hatched during the latter half of the fifth day.

scholarly journals Couple formation and spawning between two female Discus Fish (Symphysodon aequifasciatus - Cichlidae) in captivity

2017 ◽  
Vol 47 (2) ◽  
pp. 167-170
Author(s):  
Douglas da Cruz MATTOS ◽  
Rafaela SCRENCI-RIBEIRO ◽  
Leonardo Demier CARDOSO ◽  
Manuel Vazquez Vidal JUNIOR
Keyword(s):  
Embryonic Development ◽  
Reproductive Behavior ◽  
Same Sex ◽  
Same Sex Couples ◽  
Cleaning Behavior ◽  
In Captivity ◽  
Ad Libitum ◽  
Couple Formation ◽  
Substrate Cleaning

ABSTRACT In an experiment performed to characterize the reproductive behavior of the discus fish in captivity, couple formation with two females was observed. The observations were carried out in captivity, based on ad libitum methodology. Adult individuals were allowed to naturally form couples. The couple formation was considered when individuals performed the substrate cleaning behavior. Fifteen couples were selected. The eggs of three couples did not initiate embryonic development and became infeasible within three days after spawning. We found that these spawnings belonged to all-female couples. The sex of the individuals in same-sex couples was confirmed through subsequent couple formation and spawning of fertile eggs with known males. Eggs were deposited by one or both females in the same-sex couples. Double spawnings were larger and differentially colored. The motivations that lead individuals of the same sex to form couples in this species are still unknown.

scholarly journals Further observations on the potoroo (Potorous tridactylus) in captivity

1983 ◽  
Vol 17 (2) ◽  
pp. 133-137
Author(s):  
Suzanne L. Ullmann ◽  
Rena Brown
Keyword(s):  
Embryonic Development ◽  
Breeding Performance ◽  
In Captivity ◽  
Potorous Tridactylus

The maintenance and breeding performance of potoroos in captivity over a 7-year period is described. By removing offspring from the pouch and allowing 29 days for completion of embryonic development and birth to occur, pouch young of known ages can be obtained.

scholarly journals Embryonic development and larval growth of Brycon nattereri Günther, 1864 (Characidae) and its implications for captive rearing

Zygote ◽  
2017 ◽  
Vol 25 (6) ◽  
pp. 711-718 ◽  
Author(s):  
Alexandre N. Maria ◽  
Alexandre Ninhaus-Silveira ◽  
Laura H. Orfão ◽  
Ana T.M. Viveiros
Keyword(s):  
Growth Rate ◽  
Embryonic Development ◽  
Larval Growth ◽  
Mouth Opening ◽  
Yolk Sac ◽  
Larval Rearing ◽  
Initial Development ◽  
In Captivity ◽  
Artificial Fertilization ◽  
Lower Temperature

SummaryThe aim of this study was to describe, for the first time, the embryogenesis and larval growth of the Paraitinga Brycon nattereri Günther, 1864 reared in captivity. After artificial fertilization, eggs were incubated at constant temperature (~19°C) and collected every 15 min during the first 3 h and then every 3 h until hatching. Five larvae were collected daily over 15 days for evaluation of the length, yolk sac volume and specific growth rate. The following stages of embryonic development were identified: zygote, cleavage, gastrula, segmentation and larval. The hatching occurred after 50–54 h, with larvae poorly developed and fully depigmented, devoid of mouth and swimming capacity, presenting 6.32 mm total length and 3.64 mm3 yolk sac volume. The mouth opening was observed between days 3–4 after hatching. The yolk sac absorption was slow during the first 3 days, increasing sharply after this period, being completed on the day 11. During this period there was a decrease in the larval growth rate. After yolk sac absorption, an increase in the growth rate was observed that coincided with the start of exogenous feeding. Cannibalism was not observed during the 15 days of evaluation. The initial development of B. nattereri was slow and poorly developed larvae in relation to other Brycon species, certainly due to the lower temperature required for egg incubation and larval rearing. Other studies are needed in order to develop techniques to improve the methods of incubating eggs and feeding larvae.

Live Confocal Analysis of Fertilization and Early Development

2001 ◽  
Vol 7 (S2) ◽  
pp. 1012-1013
Author(s):  
Uyen Tram ◽  
William Sullivan
Keyword(s):  
Drosophila Melanogaster ◽  
Embryonic Development ◽  
Real Time ◽  
Early Development ◽  
Fluorescent Probes ◽  
Primary Defect ◽  
Fluorescent Analysis ◽  
Dynamic Event ◽  
Enormous Amount ◽  
Fluorescent Studies

Embryonic development is a dynamic event and is best studied in live animals in real time. Much of our knowledge of the early events of embryogenesis, however, comes from immunofluourescent analysis of fixed embryos. While these studies provide an enormous amount of information about the organization of different structures during development, they can give only a static glimpse of a very dynamic event. More recently real-time fluorescent studies of living embryos have become much more routine and have given new insights to how different structures and organelles (chromosomes, centrosomes, cytoskeleton, etc.) are coordinately regulated. This is in large part due to the development of commercially available fluorescent probes, GFP technology, and newly developed sensitive fluorescent microscopes. For example, live confocal fluorescent analysis proved essential in determining the primary defect in mutations that disrupt early nuclear divisions in Drosophila melanogaster. For organisms in which GPF transgenics is not available, fluorescent probes that label DNA, microtubules, and actin are available for microinjection.

Sign in / Sign up

Export Citation Format

Share Document