scholarly journals Isolation and identification of compounds with dioxin-induced AhR transformation inhibitory activity from the leaves of Mallotus japonicus (Thunb.) Muell. Arg.

2019 ◽  
Vol 13 (8) ◽  
pp. 167-172
Author(s):  
Hosokawa Keizo ◽  
Hishida Atsuyuki ◽  
Nishiumi Shin ◽  
Fukushi Eri ◽  
Kawabata Jun ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Isolation And Identification ◽  
Mallotus Japonicus

scholarly journals Mammalian Arginase Inhibitory Activity of Methanolic Extracts and Isolated Compounds from Cyperus Species

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1694
Author(s):  
Kamel Arraki ◽  
Perle Totoson ◽  
Alain Decendit ◽  
Andy Zedet ◽  
Justine Maroilley ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Methanolic Extract ◽  
Significant Inhibition ◽  
Isolation And Identification ◽  
Vasorelaxant Effect ◽  
Methanolic Extracts ◽  
Phytochemical Investigation ◽  
Ic50 Values ◽  
First Time

Polyphenolic enriched extracts from two species of Cyperus, Cyperus glomeratus and Cyperus thunbergii, possess mammalian arginase inhibitory capacities, with the percentage inhibition ranging from 80% to 95% at 100 µg/mL and 40% to 64% at 10 µg/mL. Phytochemical investigation of these species led to the isolation and identification of two new natural stilbene oligomers named thunbergin A-B (1–2), together with three other stilbenes, trans-resveratrol (3), trans-scirpusin A (4), trans-cyperusphenol A (6), and two flavonoids, aureusidin (5) and luteolin (7), which were isolated for the first time from C.thunbergii and C. glomeratus. Structures were established on the basis of the spectroscopic data from MS and NMR experiments. The arginase inhibitory activity of compounds 1–7 was evaluated through an in vitro arginase inhibitory assay using purified liver bovine arginase. As a result, five compounds (1, 4–7) showed significant inhibition of arginase, with IC50 values between 17.6 and 60.6 µM, in the range of those of the natural arginase inhibitor piceatannol (12.6 µM). In addition, methanolic extract from Cyperus thunbergii exhibited an endothelium and NO-dependent vasorelaxant effect on thoracic aortic rings from rats and improved endothelial dysfunction in an adjuvant-induced arthritis rat model.

Bioassay-Guided Isolation and Identification of a Acetylcholinesterase Inhibitors from Calophyllum polyanthum

2013 ◽  
Vol 634-638 ◽  
pp. 1225-1228 ◽  
Author(s):  
Fei Zhang ◽  
Hai Wei Ren ◽  
Yong Gang Wang ◽  
Rong Wang ◽  
Jie Sheng
Keyword(s):  
Inhibitory Activity ◽  
Cholinesterase Activity ◽  
Spectral Characteristics ◽  
Ethanolic Extract ◽  
Acetylcholinesterase Inhibitors ◽  
Microplate Assay ◽  
Acetyl Cholinesterase ◽  
Isolation And Identification ◽  
Active Compounds ◽  
Bioautographic Method

A significant acetylcholinesterase inhibitory activity was observed for the Ethanolic extract from the leaves of Calophyllum polyanthum by using TLC bioautographic method. Further bioassay-guided isolation of this extract using TLC bioautographic method resulted in obtaining a pyranochromanone, apetalic acid (1). The structure of 1 was identified by comparison of it’s spectral characteristics with previous reports. The concentration required for 50% inhibition of 1 was 0.95 mM, determined by a microplate assay. The anti-acetylcholinesterase aity of compound 1 was weak, but it was the first pyranochromanone which have anti-acetyl cholinesterase activity. As a new leading compound, it can be modified and transformed to obtain more potently active compounds.

scholarly journals Rotenoid Derivatives from Derris Trifoliata with Nitric Oxide Production Inhibitory Activity

2012 ◽  
Vol 7 (11) ◽  
pp. 1934578X1200701
Author(s):  
Chihiro Ito ◽  
Tomiyasu Murata ◽  
Hugh T.-W. Tan ◽  
Norio Kaneda ◽  
Hiroshi Furukawa ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Inhibitory Activity ◽  
Chemical Constituents ◽  
Interferon Γ ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Nitric Oxide Production ◽  
Murine Macrophage ◽  
Isolation And Identification ◽  
Oxide Production

Study of the chemical constituents of the stems of Derris trifoliata Lour. (Leguminosae) collected in Singapore led to the isolation and identification of three known and two new rotenoid derivatives. The new derivatives, named derrisfolin A (1) and B (2), inhibited nitric oxide production in murine macrophage-like RAW 264.7 cells stimulated with interferon-γ and lipopolysaccharide.

Isolation and identification of Rhizoctonia solani antagonist bacteria from the rhizosphere of pepper plants

2020 ◽  
Author(s):  
V.Zh. Nguyen ◽  
◽  
T.O. Dao ◽  
E. A. Kalashnikova ◽  
Th.H. Nguyen
Keyword(s):  
Rhizoctonia Solani ◽  
Inhibitory Activity ◽  
Soil Samples ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Phosphate Solubilizing ◽  
Solubilizing Activity ◽  
Pepper Plants

The purpose of this work is to isolate bacteria from the pepper rhizosphere that inhibit Rhizoctoniasolani and evaluate in vitro their phosphate solubilizing activity and production of siderophore. Of the different soil samples taken from the pepper fields of An Thanh, An Ninh, Quynh My, QuynhPhudistrict, ThaiBinh province, 48 bacterial strains were isolated. Of these, 5 strains (AT16, VK 4.7, VK 4.8, VK 4.12, VK 4.13) expressed as higher inhibitory Rhizoctonia solani activity were selected. Their inhibitory activity is from 11.11% to 62.22%.

scholarly journals Isolation and Identification of a Polygalacturonase Inhibiting Protein from Isatidis root

2015 ◽  
Vol 10 (2) ◽  
pp. 1934578X1501000
Author(s):  
Guanzhen Gao ◽  
Qian Wang ◽  
Jianwu Zhou ◽  
Huiqin Wang ◽  
Lijing Ke ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Inhibitory Activity ◽  
Specific Activity ◽  
Hydrophobic Interaction Chromatography ◽  
Terminal Sequence ◽  
Isolation And Identification ◽  
Polygalacturonase Inhibiting Protein ◽  
Terminal Amino ◽  
Inhibiting Protein ◽  
Cationic Exchange

Isatis indigotica Fort. (Chinese woad) has been widely used as a dye- and medicinal-plant in traditional Chinese medicine. Although I. indigotica has been cultivated in various regions across China in recent years, its innate immunity is little known. In this study, a protein with MW 37.5 kDa was purified from the extract of fresh Isatidis roots by SP-5PW cationic exchange and POROS HP2 hydrophobic interaction chromatography. The N-terminal amino acid sequence of the purified protein was subsequently determined as T-D-L-C-H-K-D-P-K-N-T-L-L by Edman degradation. The N-terminal sequence and PG inhibitory activity identified the purified substance as a polygalacturonase inhibiting protein. This purified Isatidis PGIP with a specific activity of 7.64×104 U/mg showed strong inhibitory activity against 160 U of Aspergillus niger polygalacturonase. Compared with PGIPs from guava and bean pods, Isatidis PGIP showed very poor pH and heat stabilities, which may represent the different need of plant innate immunity between plant underground and aboveground organs.

scholarly journals CHEMICAL CONSTITUENTS AND ACETYLCHOLINESTERASE ACTIVITY FROM Polyalthia sumatrana (Miq.) Kurz

2022 ◽  
Vol 52 (1) ◽  
pp. 73-76
Author(s):  
Wan Mohd Nuzul Hakimi Wan Salleh ◽  
Natasa Mohd Shakri ◽  
Mohd Azlan Nafiah ◽  
Shamsul Khamis
Keyword(s):  
Cinnamic Acid ◽  
Vanillic Acid ◽  
Inhibitory Activity ◽  
Spectroscopic Data ◽  
Chemical Constituents ◽  
Acetylcholinesterase Activity ◽  
Isolation And Identification ◽  
Acetylcholinesterase Inhibitory Activity ◽  
Reported Data

This study was carried out to investigate the phytochemicals from Polyalthia sumatrana and their acetylcholinesterase inhibitory activity. Fractionation and purification of the leaves of P. sumatrana led to the isolation and identification of five alkaloids; boldine (1), norboldine (2), liriodenine (3), predicentrine (4), laurotetanine (5) together with β-sitosterol (6), β-sitostenone (7), vanillin (8), vanillic acid (9) and cinnamic acid (10). The structures of these compounds were obtained by analysis of their spectroscopic data, as well as the comparison with that of reported data. Acetylcholinesterase inhibitory activity revealed that all isolated alkaloids were found to inhibit AChE with percentage inhibition values ranged from 45.0 to 80.6%.

Isolation of neolignan and phenolic glycosides from the branches of Viburnum macrocephalum f. keteleeri and their α-glucosidase inhibitory activity

Holzforschung ◽  
2018 ◽  
Vol 72 (12) ◽  
pp. 1017-1024 ◽  
Author(s):  
Chunchao Zhao ◽  
Jia Chen ◽  
Jianhua Shao ◽  
Jie Shen ◽  
Xiaoqing Xu ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Inhibitory Activity ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Phenolic Glycosides ◽  
Isolation And Identification ◽  
Bioassay Guided Fractionation ◽  
First Time ◽  
Deciduous Shrub

AbstractViburnum macrocephalumf.keteleeri, not well explored in the pharmaceutical field, is a tall deciduous shrub and commonly used as folk medicines for the treatment of type 2 diabetes (T2D). In this study, the dried branches ofV. macrocephalumf.keteleeriwere extracted with 95% ethanol and the extract was fractionated on the basis of polarity with ethyl acetate andn-butanol. Among the different fractions, the ethyl acetate fraction exhibited the highestα-glucosidase inhibitory activity, and its bioassay-guided fractionation led to the isolation and identification of six compounds including a new 8-O-4′-neolignan glycoside (1), categorized as a rare 7-deoxy skeleton, three previously undescribed phenolic glycosides (2–4), together with two known secondary metabolites (5,6), which were found for the first time inViburnum. Noticeably, compound1had almost the same intestinalα-glucosidase inhibitory activity as the positive control acarbose.

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