scholarly journals Effects of oligosaccharide elicitors from endophyitc Fusarium oxysporum Dzf17 on diosgenin accumulation in Dioscorea zingiberensis seedling cultures

2012 ◽  
Vol 6 (38) ◽  
pp. 5128-5134 ◽  
Author(s):  
Li Peiqin ◽  
Lou Jingfeng ◽  
Mou Yan ◽  
Sun Weibo ◽  
Shan Tijiang ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Dioscorea Zingiberensis ◽  
Oligosaccharide Elicitors

scholarly journals Enhancement of Diosgenin Production in Dioscorea zingiberensis Cell Culture by Oligosaccharide Elicitor from its Endophytic Fungus Fusarium oxysporum Dzf17

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401
Author(s):  
Ruifen Zhang ◽  
Peiqin Li ◽  
Lijian Xu ◽  
Yuanquan Chen ◽  
Peng Sui ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Fusarium Oxysporum ◽  
Cell Suspension Culture ◽  
Endophytic Fungus ◽  
Cultured Cells ◽  
Cell Suspension Cultures ◽  
Fungal Cell ◽  
Partial Acid Hydrolysis ◽  
Dioscorea Zingiberensis

Diosgenin accumulation in cell suspension cultures of Dioscorea zingiberensis C. H. Wright was enhanced by treatment with saccharide elicitors from its endophytic fungus Fusarium oxysporum isolate Dzf17. The crude oligosaccharide was prepared by partial acid hydrolysis of the isolated Dzf17 fungal cell wall fragments. Optimal elicitation of diosgenin production by the isolated Dzf17 oligosaccharide in cell suspension culture was achieved when the oligosaccharide was added to the medium at a concentration of 30 mg/L after 16-day's continuous cell suspension culture, and the cells were cultured for another 8 days before harvesting. By using these optimal conditions, the diosgenin yield of the cultured cells reached its maximum of 5.25 mg/L, which was over a three-fold increase.

scholarly journals Enhancement of Diosgenin Production in Dioscorea zingiberensis Cell Cultures by Oligosaccharides from Its Endophytic Fungus Fusarium oxysporum Dzf17

Molecules ◽  
2011 ◽  
Vol 16 (12) ◽  
pp. 10631-10644 ◽  
Author(s):  
Peiqin Li ◽  
Ziling Mao ◽  
Jingfeng Lou ◽  
Yan Li ◽  
Yan Mou ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Cell Cultures ◽  
Endophytic Fungus ◽  
Dioscorea Zingiberensis

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro)

2019 ◽  
Vol 9 (2) ◽  
pp. 91
Author(s):  
Ghea Dotulong ◽  
Stella Umboh ◽  
Johanis Pelealu
Keyword(s):  
Solanum Tuberosum ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Leaf Extract ◽  
Solanum Tuberosum L ◽  
In Vitro Toxicity ◽  
Potato Plants ◽  
Colony Diameter ◽  
In Vitro Toxicity Test

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro) Ghea Dotulong1*), Stella Umboh1), Johanis Pelealu1), 1) Program Studi Biologi, FMIPA Universitas Sam Ratulangi, Manado 95115*Email korespondensi: [email protected] Diterima 9 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kentang (Solanum tuberosum L.) adalah salah satu tanaman hortikultura yang sering mengalami penurunan dari segi produksi dan produktivitasnya, akibat adanya serangan penyakit layu yang salah satunya disebabkan oleh Fusarium oxysporum. Tujuan penelitian ini adalah mengidentifikasi toksisitas beberapa fungisida nabati dalam mengendalikan penyakit Layu Fusarium (F. oxysporum) pada tanaman kentang (Solanum tuberosum L.) secara In Vitro. Metode Penelitian yang digunakan yaitu metode umpan beracun. Data dianalisis dengan Rancangan Acak Lengkap (RAL) dengan Analisis Varian (ANAVA) yang dilanjutkan dengan menggunakan metode BNT (Beda Nyata Terkecil). Hasil Penelitian, diperoleh nilai toksisitas fungisida nabati tertinggi yaitu pada ekstrak daun sirsak dengan nilai HR (69,44%), kategori berpengaruh, ditandai dengan diameter koloni 2,75 cm (100ppm) dan yang terendah toksisitasnya yaitu pada ekstrak daun jeruk purut dengan nilai HR (49,81%), kategori cukup berpengaruh ditandai dengan diameter koloni 3,75 cm (25ppm). Semakin tinggi konsentrasi yang diujikan maka semakin tinggi toksisitas dari fungisida nabati yang diberikan.Kata Kunci: fungisida nabati, Fusarium oxysporum, tanaman kentang, In Vitro Abstract Potato plants (Solanum tuberosum L.) is one of the horticulture plants which often decreases in terms of production and productivity, due to the attack of wilt, one of which is caused by Fusarium oxysporum. The purpose of this study was to determine the toxicity of several biofungicides in controlling Fusarium wilt (F. oxysporum) in potato plants (Solanum tuberosum L.) in Vitro. The research method used was the In Vitro method with the poison bait method. Data were analyzed by Completely Randomized Design with Variant Analysis (ANAVA), followed by the BNT method. The results showed that the highest biofungicide toxicity value was soursop leaf extract with HR values (69.44%), influential categories, characterized by colony diameter 2.75 cm (100ppm) and the lowest toxicity, namely in kaffir lime leaf extract with a value of HR (49.81%), quite influential category was characterized by colony diameter of 3.75 cm (25ppm). The higher the concentration tested, the higher the toxicity of the biofungicide given.Keywords: biofungicides, Fusarium oxysporum, Potato Plants, In Vitro.

Inhabitation of Antagonistic Bacterium B96-II to Fusarium oxysporum f. sp. asparagi*

2010 ◽  
Vol 2009 (6) ◽  
pp. 861-865 ◽  
Author(s):  
Liping MA ◽  
Bianqing HAO ◽  
Jing WANG ◽  
Shu QIN ◽  
Xiongwu QIAO
Keyword(s):  
Fusarium Oxysporum ◽  
Antagonistic Bacterium

Effects of Fusarium oxysporum f. sp. cubense race 4 on microorganisms and enzyme activities in the rhizosphere soil of banana

2013 ◽  
Vol 38 (2) ◽  
pp. 173-176 ◽  
Author(s):  
Yong-hong HUANG ◽  
Shun LÜ ◽  
Chun-yu LI ◽  
Yue-rong WEI ◽  
Gan-jun YI
Keyword(s):  
Fusarium Oxysporum ◽  
Enzyme Activities ◽  
Rhizosphere Soil ◽  
Race 4

A Regeneration System and Ploidy Identification of Plantlets for Endosperm of Dioscorea zingiberensis

2013 ◽  
Vol 47 (6) ◽  
pp. 654-660
Author(s):  
Li Mingjun ◽  
Guo Jing ◽  
Li Xiang ◽  
Li Jiqiang ◽  
Wang Yipeng ◽  
...  
Keyword(s):  
Regeneration System ◽  
Dioscorea Zingiberensis ◽  
Ploidy Identification
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