scholarly journals Molecular diversity of arbuscular mycorrhizal fungi (AMF) in Lake Victoria Basin of Kenya

2014 ◽  
Vol 6 (4) ◽  
pp. 145-152 ◽  
Author(s):  
J. O Othira ◽  
O. Omolo J. ◽  
Kiruki S. ◽  
A. Onek L. ◽  
N. Wachira F.
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Molecular Diversity ◽  
Lake Victoria ◽  
Arbuscular Mycorrhizal ◽  
Lake Victoria Basin

scholarly journals Molecular Diversity of Arbuscular Mycorrhizal Fungi (AMF) Associated with Carissa edulis, an Endangered Plant Species along Lake Victoria Basin of Kenya

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Benard O. Ogoma ◽  
Stephen F. Omondi ◽  
Jane Ngaira ◽  
Josephine W. Kimani
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Lake Victoria ◽  
Herbal Medicines ◽  
Arbuscular Mycorrhizal ◽  
Small Subunit ◽  
Morphological Identification ◽  
Lake Victoria Basin ◽  
Cross Sectional ◽  
Significant Difference

Carissa edulis is a tropical plant belonging to the family Apocynaceae. The species is widely used in the preparation of various herbal medicines. Earlier works in Kenya show that an aqueous extract from the roots of C. edulis has remarkable anti-herpes simplex virus. Due to its medicinal value, the species has been overexploited in its natural range and requires conservation interventions. Studies show that the species has beneficial relationships with arbuscular mycorrhizal fungi (AMF) that can enhance restoration of its population; however, no study has been undertaken to document the diversity of these AMF species. This study evaluated the genetic diversity of AMF associated with the roots of C. edulis within Lake Victoria basin ecosystem of Kenya. A cross-sectional, laboratory-based prospective study was carried out from roots of C. edulis collected from six sites within the ecosystem. Root samples were collected from 6 points (replicates) per site. AMF was assessed through morphological characterization and sequencing of small subunit of ribosomal DNA. Morphological identification identified four genera of AMF (Gigaspora, Acaulospora, Scutellospora, and Glomus) with no significant difference among the sites. Molecular analysis also revealed presence of four genera, but only two (Glomus and Acaulospora) were common for both the analyses with Glomus as the most predominant genera. In all the sites, there were large numbers of spores both in soil and in the roots confirming the association between C. edulis and AMF.

Morphology and molecular diversity of arbuscular mycorrhizal fungi in wild and cultivated yew (Taxus baccata)

2003 ◽  
Vol 81 (3) ◽  
pp. 255-266 ◽  
Author(s):  
Tesfaye Wubet ◽  
Michael Weiß ◽  
Ingrid Kottke ◽  
Franz Oberwinkler
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Molecular Diversity ◽  
Arbuscular Mycorrhizal ◽  
Molecular Techniques ◽  
Internal Transcribed Spacers ◽  
Taxus Baccata ◽  
Data Set ◽  
Wide Range ◽  
Sequence Types

Taxus baccata L. roots collected from two sites in southern Germany were heavily colonized by arbuscular mycorrhizal fungi (AMF). The colonization pattern was of the Paris type. The diversity of the colonizing AMF species was investigated using polymerase chain reaction based molecular techniques. The internal transcribed spacer (ITS) region of the DNA from AMF within the roots was amplified using Glomeromycota-specific primers and then cloned and sequenced. Phylogenetic analysis using a data set of 5.8S rDNA sequences from a wide range of glomeralean taxa as well as data sets of partial ITS2 sequences from glomeralean subgroups indicated root colonization by four sequence types of Glomus and one sequence type of Archaeospora. These sequence types are distinct from any previously published sequences and differed between the two study sites.Key words: arbuscular mycorrhiza, Paris-type AM, molecular diversity, ribosomal internal transcribed spacers, Taxus baccata.

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