Evaluation of four serological tests to detect prevalence of bovine brucellosis in Khartoum State

2012 ◽  
Vol 6 (9) ◽  
Author(s):  
Adil M. A. Salman
Keyword(s):  
Bovine Brucellosis ◽  
Serological Tests

scholarly journals A quantitative comparison of the sensitivity of serological tests for bovine brucellosis to different antibody classes

1976 ◽  
Vol 76 (2) ◽  
pp. 287-298 ◽  
Author(s):  
G. S. Allan ◽  
R. J. Chappel ◽  
P. Williamson ◽  
D. J. McNaught
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Quantitative Comparison ◽  
Agglutination Test ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Plate Test ◽  
Specific Antibodies ◽  
The Rose ◽  
Serum Agglutination

SUMMARYBrucella-specific antibodies of different immunoglobulin classes were quantitatively evaluated with respect to their efficiency in serological tests for bovine brucellosis.IgM reacted more efficiently than IgG1and IgG2in both the Rose Bengal plate test and serum agglutination test. The complement fixation test was found to be slightly more sensitive to IgM than to IgG1and did not react to IgG2.IgM was, however, partly inactivated when heated at 60°C. in the presence of serum.

scholarly journals Bayesian evaluation of three serological tests for the diagnosis of bovine brucellosis in Bangladesh

2019 ◽  
Vol 147 ◽  
Author(s):  
A. K. M. A. Rahman ◽  
S. Smit ◽  
B. Devleesschauwer ◽  
P. Kostoulas ◽  
E. Abatih ◽  
...  
Keyword(s):  
Latent Class ◽  
Latent Class Model ◽  
Dairy Farm ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bovine Brucellosis ◽  
Class Model ◽  
Serological Tests ◽  
Predictive Values ◽  
Conditional Dependence

AbstractWe evaluated the performance of three serological tests – an immunoglobulin G indirect enzyme linked immunosorbent assay (iELISA), a Rose Bengal test and a slow agglutination test (SAT) – for the diagnosis of bovine brucellosis in Bangladesh. Cattle sera (n = 1360) sourced from Mymensingh district (MD) and a Government owned dairy farm (GF) were tested in parallel. We used a Bayesian latent class model that adjusted for the conditional dependence among the three tests and assumed constant diagnostic accuracy of the three tests in both populations. The sensitivity and specificity of the three tests varied from 84.6% to 93.7%, respectively. The true prevalences of bovine brucellosis in MD and the GF were 0.6% and 20.4%, respectively. Parallel interpretation of iELISA and SAT yielded the highest negative predictive values: 99.9% in MD and 99.6% in the GF; whereas serial interpretation of both iELISA and SAT produced the highest positive predictive value (PPV): 99.9% in the GF and also high PPV (98.9%) in MD. We recommend the use of both iELISA and SAT together and serial interpretation for culling and parallel interpretation for import decisions. Removal of brucellosis positive cattle will contribute to the control of brucellosis as a public health risk in Bangladesh.

Field trial of six serological tests for bovine brucellosis

2012 ◽  
Vol 191 (3) ◽  
pp. 364-370 ◽  
Author(s):  
D.A. Abernethy ◽  
F.D. Menzies ◽  
S.J. McCullough ◽  
S.W.J. McDowell ◽  
K.E. Burns ◽  
...  
Keyword(s):  
Field Trial ◽  
Bovine Brucellosis ◽  
Serological Tests

scholarly journals Efficacy of Several Serological Tests and Antigens for Diagnosis of Bovine Brucellosis in the Presence of False-Positive Serological Results Due to Yersinia enterocolitica O:9

2005 ◽  
Vol 12 (1) ◽  
pp. 141-151 ◽  
Author(s):  
P. M. Muñoz ◽  
C. M. Marín ◽  
D. Monreal ◽  
D. González ◽  
B. Garin-Bastuji ◽  
...  
Keyword(s):  
Yersinia Enterocolitica ◽  
False Positive ◽  
Lipid A ◽  
Protein Complexes ◽  
Serological Test ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Cytosolic Proteins ◽  
Membrane Protein Complexes ◽  
Sensitivity Specificity

ABSTRACT Yersinia enterocolitica O:9 bears a smooth lipopolysaccharide (S-LPS) of Brucella sp. O-chain A + C/Y epitopic structure and is a cause of false-positive serological reactions (FPSR) in standard tests for cattle brucellosis. Brucella S-LPS, cross-reacting S-LPSs representing several O-chain epitope combinations, Brucella core lipid A epitopes (rough LPS), Brucella abortus S-LPS-derived polysaccharide, native hapten polysaccharide, rough LPS group 3 outer membrane protein complexes, recombinant BP26, and cytosolic proteins were tested in enzyme-linked immunosorbent assays (ELISA) and precipitation tests to detect cattle brucellosis (sensitivity) and to differentiate it from FPSR (specificity). No single serological test and antigen combination showed 100% sensitivity and specificity simultaneously. Immunoprecipitation tests with native hapten polysaccharide, counterimmunoelectrophoresis with cytosolic proteins, and a chaotropic ELISA with Brucella S-LPS were 100% specific but less sensitive than the Rose Bengal test, complement fixation, and indirect ELISA with Brucella S-LPSs and native hapten or S-LPS-derived polysaccharides. A competitive ELISA with Brucella S-LPS and M84 C/Y-specific monoclonal antibody was not 100% specific and was less sensitive than other tests. ELISA with Brucella suis bv. 2 S-LPS (deficient in C epitopes), Escherichia hermannii S-LPSs [lacking the contiguous α-(1-2)-linked perosamine residues characteristic of Y. enterocolitica S-LPS], BP26 recombinant protein, and Brucella cytosolic fractions did not provide adequate sensitivity/specificity ratios. Although no serological test and antigen combination fully resolved the diagnosis of bovine brucellosis in the presence of FPSR, some are simple and practical alternatives to the brucellin skin test currently recommended for differential diagnosis.

scholarly journals Whole-blood lymphocyte stimulation assay for measurement of cell-mediated immune responses in bovine brucellosis

1978 ◽  
Vol 7 (6) ◽  
pp. 550-557
Author(s):  
J M Kaneene ◽  
R K Anderson ◽  
D W Johnson ◽  
C C Muscoplat ◽  
P Nicoletti ◽  
...  
Keyword(s):  
Immune Response ◽  
Whole Blood ◽  
Blood Lymphocyte ◽  
Lymphocyte Stimulation ◽  
Soluble Antigen ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Cell Mediated Immune Response ◽  
Stimulation Tests

A study was conducted to develop an in vitro whole-blood lymphocyte stimulation assay for measurement of cell-mediated immune response in bovine brucellosis. A soluble antigen (BASA) prepared from killed cells of Brucella abortus 1119-3 was used. Cattle infected with B. abortus field strains, B. abortus 19 calfhood- and adult-vaccinated cattle, and nonexposed cattle were tested. Blood was diluted 10-fold in RPMI-1640 medium (without added serum) and cultured with BASA (at a concentration of 2.2 microgram per culture) at varying times of incubation. Results were assayed for [3H]thymidine incorporation into deoxyribonucleic acid. A 6-day period was found to be optimal for incubating blood cultures to achieve maximum specific lymphocyte stimulation. Serological tests and bacteriological isolation attempts were conducted simultaneously with lymphocyte stimulation tests, and there was a significant correlation between cell-mediated immune response and bacteriological findings. There was a significant correlation between cell-mediated immune response and the level of serum antibodies on a group basis, but there was little correlation between the two systems on individual infected animals. Among vaccinated animals there was little or no correlation between cell-mediated immune and humoral responses. The whole-blood assay was found to be simple, fast, sensitive, and reproducible.

scholarly journals Seroprevalence of brucellosis and molecular characterization of Brucella spp. from slaughtered cattle in Rwanda

2021 ◽  
Author(s):  
Jean Bosco Ntivuguruzwa ◽  
Francis Babaman Kolo ◽  
Emil Ivan Mwikarago ◽  
Henriette vanHeerden
Keyword(s):  
Control Program ◽  
Culture Method ◽  
Cross Sectional Study ◽  
Selective Medium ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Cross Sectional ◽  
Standard Culture

AbstractBovine brucellosis is endemic in Rwanda, although, there is paucity of documented evidence about the disease in slaughtered cattle. A cross-sectional study was conducted in slaughtered cattle (n=300) to determine the seroprevalence of anti-Brucella antibodies using the Rose Bengal Test (RBT), and indirect enzyme-linked immunosorbent assay (i-ELISA). Corresponding tissues were cultured onto a modified Centro de Investigación y Tecnología Agroalimentaria (CITA) selective medium and analysed for Brucella spp. using the 16S-23S ribosomal interspacer region (ITS), AMOS, and Bruce-ladder PCR assays. The RBT seroprevalence was 20.7% (62/300), and 2.9% (8/300) with i-ELISA and 2.9% (8/300) using both tests in parallel. Brucella specific 16S-23S ribosomal DNA interspace region (ITS) PCR detected Brucella DNA in 5.6% (17/300; Brucella culture prevalence). AMOS-PCR assay identified mixed B. abortus and B. melitensis (n=3), B. abortus (n=3) and B. melitensis (n=5) while Bruce-ladder PCR also identified B. abortus (n=5) and B. melitensis (n=6). The gold standard culture method combined with PCR confirmation identified 5.6% Brucella cultures which is higher than the more sensitive seroprevalence of 2.9%. This emphasizes the need to validate the serological tests in Rwanda. The mixed infection caused by B. abortus and B. melitensis in slaughtered cattle indicates cross-infection and poses a risk of exposure potential to abattoir workers. It is essential to urgently strengthen the national bovine brucellosis control program through vaccination as well as test- and-slaughter.

scholarly journals The diagnostic efficiency of some serological tests for bovine brucellosis

1978 ◽  
Vol 80 (3) ◽  
pp. 373-384 ◽  
Author(s):  
R. J. Chappel ◽  
D. J. McNaught ◽  
J. A. Bourke ◽  
G. S. Allan
Keyword(s):  
False Positive ◽  
False Negative ◽  
Diagnostic System ◽  
Diagnostic Value ◽  
Diagnostic Efficiency ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Probable Infection ◽  
Serum Agglutination

SummaryResults obtained from 1887 sera using three serological tests for bovine brucellosis were compared with a serological classification of sera described as the ‘probable infection status’. Sera showing apparent false positive and apparent false negative reactions were identified, and were subjected to supplementary testing as appropriate.The serum agglutination test (SAT) gave 35% apparent false negative reactions and 5% apparent false positives. The complement fixation test (CFT) gave 12% apparent false negative reactions using warm fixation (CFTW) and at least 5% using cold fixation (CFTC). The routine diagnostic system used in Victoria, in which the CFTW is supplemented by the CFTC and the SAT, gave 9% apparent false negative reactions and 2% apparent false positive reactions. The radioimmunoassay gave 1% or 6% apparent false negative reactions, depending the minimum diagnostic value used.Atypical reactions in the CFT sometimes caused difficulties in diagnosis.

scholarly journals Examination of sensitivity and specificity of some serological tests in diagnostics of bovine brucellosis

2008 ◽  
Vol 58 (5-6) ◽  
pp. 467-476 ◽  
Author(s):  
Matovic Kazimir ◽  
Asanin Ruzica ◽  
Radojicic Sonja ◽  
Lako B. ◽  
Zarkovic A.
Keyword(s):  
Sensitivity And Specificity ◽  
Bovine Brucellosis ◽  
Serological Tests
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