scholarly journals Potential of cassava starch from TME 419 as suitable gelling agent in micropropagation of cassava (Manihot esculenta Crantz)

2017 ◽  
Vol 11 (12) ◽  
pp. 403-407
Author(s):  
K. NKERE Chukwuemeka ◽  
IKPEAMA Ahamefula ◽  
O. UZOECHI Amarachukwu ◽  
N. A. MBANASO Egbichi
2010 ◽  
Vol 30 (1) ◽  
pp. 213-217 ◽  
Author(s):  
Roberto do Nascimento Silva ◽  
Fábio Pereira Quintino ◽  
Valdirene Neves Monteiro ◽  
Eduardo Ramirez Asquieri

The high demands for sugars and the development of enzymatic technology have increased the production of sweeteners, especially for glucose and fructose syrups. This work describe a technology for glucose and fructose syrups from Brazilian cassava starch using enzymes produced by soil microrganisms isolated from the Brazilian Cerrado soil. Firstly, Aspergillus niger and Streptomyces sp. were isolated from the soil and used as glucoamylase (GA) and glucose isomerase (GI) producer sources. After characterization, GA and GI exhibited optimum pH 4.5 and 8.0, respectively. GA showed maximum activity at 60 ºC and GI at 85 ºC. GA and GI retained 65 and 80%, respectively, of initial activity after 180 minutes of incubation at 60 ºC. The kinetic parameters Km and Vmáx were 0.476 (mg.mL-1) and 8.58 (µmol/minute) for GA and 0.082 (M) and 48.20 (µmol/minute) for GI. The maximum glucose syrups production occurred after 24 hours of reaction with a 98% yield. The production of fructose syrups with 42% (w/v) was reached after 96 hours of reaction.


2007 ◽  
Vol 78 (4) ◽  
pp. 1212-1220 ◽  
Author(s):  
F. Martínez-Bustos ◽  
M. López-Soto ◽  
E. San Martín-Martínez ◽  
J.J. Zazueta-Morales ◽  
J.J. Velez-Medina

2020 ◽  
Vol 8 (10) ◽  
pp. 237-244
Author(s):  
Duru Maduabuchi ◽  
◽  
Mbata Ikechukwu ◽  
Osikwe Keziah ◽  
Ukaoma Adamma ◽  
...  

The study investigated an in vitro propagation of Manihot esculenta Crantz in a substituted substrate regime. The aim was to proffer and affordable alternative to the expensive high tech media formulations usually employed in tissue culture protocol. The experiment was conducted on laboratory bench, using standard tissue culture and micropropagation methods under aseptic conditions. The morphogenesis effect of the substrate was determined based on the integer number of explants’ callus and adventitious shoot regeneration. Results showed that MS + Agar, supported embryogenic callus formation with 38% viability, NH4NO3 + KH2PO4 + Agar, supported same with 29%. MS + 2, 4-D + BAP +Agar supported shoot establishment with 32%. While NH4NO3 + KH2PO4 + Zea mays extracts + Agar, did same with 43.26%. MS + Soil, supported callugenesis with 27% viability while NH4NO3 + KH2PO4 + Soil supported the callus establishment with 25%. MS + 2,4 - D + BAP + Soil, supported shoot establishment with 38.41% viability while NH4NO3 + KH2PO4 + Zea mays Extracts + Soil supported same with 36%. The application of crude Zea mays seedling extracts can serve as potent alternative to the synthetic 2, 4 – D and BAP, in in vitro somatic cell morphogenesis. NH4NO3 + KH2 + PO4 can substitute for the MS salt in the same protocol. Loamy top soil can be a good alternative to agar powder as gelling agent in cassava somatic cell embryogenesis and shoot regeneration. Keywords: Ammonium nitrate, Potassium biphosphate, MS salt, axillary meristem, morphogenesis.


2011 ◽  
Vol 63 (10) ◽  
pp. 595-603 ◽  
Author(s):  
Renata Baraldi de Pauli ◽  
Leda Battestin Quast ◽  
Ivo Mottin Demiate ◽  
Lyssa Setsuko Sakanaka

2018 ◽  
Vol 63 (1) ◽  
pp. 11-17
Author(s):  
Hargono Hargono ◽  
Andri Cahyo Kumoro ◽  
Bakti Jos

The kinetics and inhibitory effects of cyanide on the granular starch hydrolyzing enzyme (GSHE) activity during hydrolysis of cassava (Manihot esculenta Crantz) starch at low temperature were studied. The substrates included native cassava starch at various concentrations (100-400 g/L) and native cassava starches with added cyanide at various concentrations (50-150 mg/kg), while the concentration of enzyme was 1.5% (w/w). A decrease in reducing sugar concentration during hydrolysis of cassava starch indicated that the cyanide reduced the enzyme activity. Lineweaver-Burk plot of Michaelis-Menten equation was used to study the inhibition kinetics. The maximum velocity (Vmax) value was higher for native cassava starch than that of native cassava starch with added cyanides. The presence of cyanide was found to reduce the Vmax values. No significant different of the saturation constant (Km) value between native cassava starch and native cassava starch with added cyanides was observed. Based on the inhibition type analysis, the effect of cyanide in the cassava starch can be classified as a noncompetitive inhibition, with the Ki value of 0.33 mg/L.


2019 ◽  
Vol 19 (1) ◽  
pp. 78
Author(s):  
Ardiansyah Ardiansyah ◽  
Endang Sri Wahyuni

This study aims to determine the effect of heating on physicochemical properties of cassava flour by modifying the heating and organoleptic of cassava rice with cassava flour modified heating. The experimental design used in this study was a completely randomized design, consisting of one factor with six levels of treatment namely 0 minutes warm-up time, 15 minutes, 30 minutes, 45 minutes 60 minutes and 75 minutes. The results of the study data were analyzed by anova test and carried out by the Tukey HSD test. From the results of the study it was concluded that there was a warming effect on digestibility, cassava starch resistant starch modified heating. Cassava rice with cassava flour modified heating is not liked by panelists.


2018 ◽  
Vol 4 (3) ◽  
pp. 133
Author(s):  
T Ariani Widiastini ◽  
Elly Nurus Sakinah ◽  
Yudha Nurdian ◽  
Jauhar Firdaus

Type 2 Diabetes Mellitus (T2DM) is a condition in which both insulin secretion and insulin sentivity disturbed. One of therapeutic approach of T2DM is through diet modification using resistant starch (RS) that has proven controlling both postprandial and fasting blood glucose. Cassava starch has 28,57% amylose and 51,24% amylopectin that can be increased through autoclaving-cooling process. Therefore, it can resist the digestion proess by amylose enzyme. The aim was to describe the effect of type 3 resistant starch cassava (Manihot esculenta Crantz) on fasting blood glucose (FBG) in Wistar diabetic rat models. This study was quasy experimental post-test only control group design using 16 Wistar rats divided into 4 groups, KN (normal), K- (DM), P1 (DM + cassava starch diet), and P2 (DM + type 3 RS cassava diet). High fat diet (HFD) and low dose streptozotocin (STZ) used as diabetic agents. Rats fed cassava starch or type 3 RS cassava diet for 28 days. GOD-PAP method used to measure the FBG level. The data analyzed using One Way ANOVA test with p>0,05 as significant value. The result shows no significant differences within experimental groups. Keywords: cassava, autoclaving-cooling, resistant starch, FBG


Author(s):  
Víctor Ortiz Alcocer ◽  
Gaspar López Ocaña ◽  
Carlos Alberto Torres Balcazar ◽  
Liliana Pampillón González

La coagulación floculación se ha empleado en el tratamiento de aguas residuales utilizando sales metálicas y polímeros con el fin de remover sólidos en suspensión, entre otros contaminantes. La turbiedad y el color son indicadores de sólidos suspendidos en el agua residual, y como parámetros de control es esencial determinarlos debido a que representan pruebas rápidas para precisar la reducción de los sólidos (suspendidos y disueltos). En tal sentido, en este estudio se evaluó la eficiencia de remoción de dichos parámetros de un agua residual doméstica, en la cual se aplicó un tratamiento de coagulación-floculación agregando el polímero natural almidón de yuca (Manihot esculenta Crantz) y combinando coagulantes floculantes como Al2(SO4)3, FeCl3 y Ca(OH)2, de modo que se pudieran hallar las dosis óptimas de polímero y coagulantes para ofrecer la mejor remoción de turbiedad y color. En total, se analizaron 216 muestras de agua residual doméstica: 54 para determinar la dosis óptimas de los coagulantes y 162 de las mezclas. La combinación que presentó la mayor eficiencia fue la combinación de 250 mgL-1 de Al2(SO4)3 y 750 mgL-1 del almidón, con turbiedad inicial de 24 NTU y color inicial de 958 CU. Esta logró remover 75 % de la turbiedad y 78 % del color, y obtuvo una turbiedad final de 6 NTU y color final de 210 CU. La segunda mezcla fue 250 mgL-1 de Ca(OH)2 y 250 mgL-1 de almidón, con turbiedad inicial de 23.9 NTU y color inicial de 1430 CU. Esta combinación removió 34.3 % de turbiedad y 67.6 % del color, es decir, se redujo la turbiedad hasta 15.7 NTU y el color hasta 453 CU. El ahorro en el consumo de sulfato de aluminio para este tipo de agua residual fue de 16.7 %, ya que la dosis del reactivo fue de 300 mgL-1, mientras que para el hidróxido de calcio el ahorro en el reactivo fue de 37.5 %, debido a que la dosis óptima de este estuvo en 400 mgL-1.


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