In vivo anti-inflammatory and in vitro antioxidant properties of Hilleria latifolia and Laportea ovalifolia

2016 ◽  
Vol 10 (20) ◽  
pp. 442-450
Author(s):  
Oteng Dapaah Susana ◽  
Agyare Christian ◽  
Darko Obiri David ◽  
Duah Boakye Yaw ◽  
Osafo Newman
Keyword(s):  
Antioxidant Properties ◽  
Anti Inflammatory

scholarly journals In Vitro Evaluation of the Anti-Inflammatory Effect of KMUP-1 and in Vivo Analysis of Its Therapeutic Potential in Osteoarthritis

Biomedicines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 615
Author(s):  
Shang-En Huang ◽  
Erna Sulistyowati ◽  
Yu-Ying Chao ◽  
Bin-Nan Wu ◽  
Zen-Kong Dai ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Inflammatory Responses ◽  
Therapeutic Potential ◽  
Antioxidant Properties ◽  
Serum Levels ◽  
Gene Expressions ◽  
Tnf Α ◽  
Anti Inflammatory

Osteoarthritis is a degenerative arthropathy that is mainly characterized by dysregulation of inflammatory responses. KMUP-1, a derived chemical synthetic of xanthine, has been shown to have anti-inflammatory and antioxidant properties. Here, we aimed to investigate the in vitro anti-inflammatory and in vivo anti-osteoarthritis effects of KMUP-1. Protein and gene expressions of inflammation markers were determined by ELISA, Western blotting and microarray, respectively. RAW264.7 mouse macrophages were cultured and pretreated with KMUP-1 (1, 5, 10 μM). The productions of TNF-α, IL-6, MMP-2 and MMP- 9 were reduced by KMUP-1 pretreatment in LPS-induced inflammation of RAW264.7 cells. The expressions of iNOS, TNF-α, COX-2, MMP-2 and MMP-9 were also inhibited by KMUP-1 pretreatment. The gene expression levels of TNF and COX families were also downregulated. In addition, KMUP-1 suppressed the activations of ERK, JNK and p38 as well as phosphorylation of IκBα/NF-κB signaling pathways. Furthermore, SIRT1 inhibitor attenuated the inhibitory effect of KMUP-1 in LPS-induced NF-κB activation. In vivo study showed that KMUP-1 reduced mechanical hyperalgesia in monoiodoacetic acid (MIA)-induced rats OA. Additionally, KMUP-1 pretreatment reduced the serum levels of TNF-α and IL-6 in MIA-injected rats. Moreover, macroscopic and histological observation showed that KMUP-1 reduced articular cartilage erosion in rats. Our results demonstrated that KMUP-1 inhibited the inflammatory responses and restored SIRT1 in vitro, alleviated joint-related pain and cartilage destruction in vivo. Taken together, KMUP-1 has the potential to improve MIA-induced articular cartilage degradation by inhibiting the levels and expression of inflammatory mediators suggesting that KMUP-1 might be a potential therapeutic agent for OA.

scholarly journals Mitoprotective Effects of Centella asiatica (L.) Urb.: Anti-Inflammatory and Neuroprotective Opportunities in Neurodegenerative Disease

2021 ◽  
Vol 12 ◽  
Author(s):  
Jia Hui Wong ◽  
Anna M. Barron ◽  
Jafri Malin Abdullah
Keyword(s):  
Neurodegenerative Disease ◽  
Healthy Aging ◽  
Brain Aging ◽  
Antioxidant Properties ◽  
Centella Asiatica ◽  
Asiatic Acid ◽  
Wide Range ◽  
Anti Inflammatory

Natural products remain a crucial source of drug discovery for accessible and affordable solutions for healthy aging. Centella asiatica (L.) Urb. (CA) is an important medicinal plant with a wide range of ethnomedicinal uses. Past in vivo and in vitro studies have shown that the plant extract and its key components, such as asiatic acid, asiaticoside, madecassic acid and madecassoside, exhibit a range of anti-inflammatory, neuroprotective, and cognitive benefits mechanistically linked to mitoprotective and antioxidant properties of the plant. Mitochondrial dysfunction and oxidative stress are key drivers of aging and neurodegenerative disease, including Alzheimer’s disease and Parkinson’s disease. Here we appraise the growing body of evidence that the mitoprotective and antioxidative effects of CA may potentially be harnessed for the treatment of brain aging and neurodegenerative disease.

Anti-inflammatory, Antinociceptive, and Antioxidant Potentials of Algerian Zizyphus jujuba Mill. Fruits and Leaves In Vivo and In Vitro in Different Experimental Models

2019 ◽  
Author(s):  
N. Gadiri ◽  
C. Tigrine ◽  
Z. Lakache ◽  
A. Kameli
Keyword(s):  
Antioxidant Properties ◽  
Experimental Models ◽  
Protective Capacity ◽  
Aerial Parts ◽  
Antioxidant Agents ◽  
Zizyphus Jujuba ◽  
Anti Inflammatory ◽  
Dpph Scavenging

The ethanolic extracts of the aerial parts, leaves, and fruits, of Zizyphus jujuba Mill. were studied in vivo and in vitro for their anti-inflammatory, analgesic, and antioxidant properties, respectively. A preliminary phytochemical screening was carried out. It revealed the presence of several phytoconstituents such as flavonoids, phenols, and alkaloids. The acute toxicity study was conducted using a limit dose of 2,000mg/kg. The extracts studied showed no signs of toxicity or mortality. Anti-inflammatory activity, assessed by carrageenan-induced paw edema, and antinociceptive activity, assessed by pain-induced acetic acid in mice, were significantly reduced (P < 0.001). Moreover, both extracts showed considerable DPPH scavenging activity and β-carotene protective capacity. The present study suggests that leaf and fruit extracts from Zizyphus jujuba have a pharmacological interest probably related to the presence of some bioactive compounds and could be a potential source of antiinflammatory, antinociceptive, and antioxidant agents that should be further investigated.

scholarly journals Evaluation of Anti-Inflammatory Properties of Isoorientin Isolated from Tubers of Pueraria tuberosa

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Kotha Anilkumar ◽  
Gorla V. Reddy ◽  
Rajaram Azad ◽  
Nagendra Sastry Yarla ◽  
Gangappa Dharmapuri ◽  
...  
Keyword(s):  
Cell Line ◽  
Antioxidant Properties ◽  
Model Systems ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Pueraria Tuberosa ◽  
Cox 2 ◽  
Anti Inflammatory

Inflammation is the major causative factor of different diseases such as cardiovascular disease, diabetes, obesity, osteoporosis, rheumatoid arthritis, inflammatory bowel disease, and cancer. Anti-inflammatory drugs are often the first step of treatment in many of these diseases. The present study is aimed at evaluating the anti-inflammatory properties of isoorientin, a selective cyclooxygenase-2 (COX-2) inhibitor isolated from the tubers of Pueraria tuberosa, in vitro on mouse macrophage cell line (RAW 264.7) and in vivo on mouse paw edema and air pouch models of inflammation. Isoorientin reduced inflammation in RAW 264.7 cell line in vitro and carrageenan induced inflammatory animal model systems in vivo. Cellular infiltration into pouch tissue was reduced in isoorientin treated mice compared to carrageenan treated mice. Isoorientin treated RAW 264.7 cells and animals showed reduced expression of inflammatory proteins like COX-2, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), 5-lipoxygenase (5-LOX), and interleukin 1-β (IL-1-β) both in vitro and in vivo. The antioxidant enzyme levels of catalase and GST were markedly increased in isoorientin treated mice compared to carrageenan treated mice. These results suggest that isoorientin, a selective inhibitor of COX-2, not only exerts anti-inflammatory effects in LPS induced RAW cells and carrageenan induced inflammatory model systems but also exhibits potent antioxidant properties.

scholarly journals IN VITRO ANTIOXIDANT ACTIVITY, IN VIVO ANTIPYRETIC AND ANTI-INFLAMMATORY ACTIVITIES OF PARONYCHIA CAPITATA L.

2017 ◽  
Vol 10 (3) ◽  
pp. 177
Author(s):  
Soumia Bouzidi ◽  
Benkiki Naima ◽  
Allaoua Zina
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Butanol Extract ◽  
Antipyretic Activity ◽  
Male Wistar Rats ◽  
Anti Inflammatory ◽  
In Vitro Antioxidant Activity

ABSTRACTObjective: The aim of this study is to evaluate the in vitro antioxidant activity of Paronychia capitata various extracts, acute toxicity, the in vivoantipyretic and anti-inflammatory activities of the n-butanol extract.Methods: The antioxidant properties were tested using 2,2-diphenyl 1-picrylhydrazyl radical scavenging and β-carotene linoleic acid system, thetotal phenolic and flavonoid contents were determined. Furthermore, anti-inflammatory activity and antipyretic activity of the n-butanol extractwere assessed on male Wistar rats at the dose levels 250, 500 mg/kg body weight, using the egg albumin-induced edema, and brewer’s yeast-inducedpyrexia method, respectively.Results: The n-butanol extract of P. capitata had considerably the highest antiradical activity with inhibitory concentration 50 value of 8.667 μg/ml.Conclusion: The experimental data demonstrated that n-butanol extract of P. capitata possess significantly remarkable anti-inflammatory andantipyretic activities compared to the standard drugs.Keywords: Caryophyllaceae, Flavonoid, Inflammation, Fever, Paronychia capitata.

scholarly journals Fucoxanthin-Containing Cream Prevents Epidermal Hyperplasia and UVB-Induced Skin Erythema in Mice

Marine Drugs ◽  
2018 ◽  
Vol 16 (10) ◽  
pp. 378 ◽  
Author(s):  
Azahara Rodríguez-Luna ◽  
Javier Ávila-Román ◽  
María González-Rodríguez ◽  
María Cózar ◽  
Antonio Rabasco ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Antioxidant Properties ◽  
Epidermal Hyperplasia ◽  
Topical Formulation ◽  
Tnf Α ◽  
Skin Erythema ◽  
Cox 2 ◽  
Anti Inflammatory

Microalgae represent a source of bio-active compounds such as carotenoids with potent anti-inflammatory and antioxidant properties. We aimed to investigate the effects of fucoxanthin (FX) in both in vitro and in vivo skin models. Firstly, its anti-inflammatory activity was evaluated in LPS-stimulated THP-1 macrophages and TNF-α-stimulated HaCaT keratinocytes, and its antioxidant activity in UVB-irradiated HaCaT cells. Next, in vitro and ex vivo permeation studies were developed to determine the most suitable formulation for in vivo FX topical application. Then, we evaluated the effects of a FX-containing cream on TPA-induced epidermal hyperplasia in mice, as well as on UVB-induced acute erythema in hairless mice. Our results confirmed the in vitro reduction of TNF-α, IL-6, ROS and LDH production. Since the permeation results showed that cream was the most favourable vehicle, FX-cream was elaborated. This formulation effectively ameliorated TPA-induced hyperplasia, by reducing skin edema, epidermal thickness, MPO activity and COX-2 expression. Moreover, FX-cream reduced UVB-induced erythema through down-regulation of COX-2 and iNOS as well as up-regulation of HO-1 protein via Nrf-2 pathway. In conclusion, FX, administered in a topical formulation, could be a novel natural adjuvant for preventing exacerbations associated with skin inflammatory pathologies as well as protecting skin against UV radiation.

scholarly journals Evaluation of Antioxidant, Anti-inflammatory and Analgesic Activities of Leaves of Saba senegalensis (A.DC) Pichon (Apocynaceae)

2019 ◽  
pp. 1-12
Author(s):  
Mohamed Bonewendé Belemlilga ◽  
Tata Kady Traoré ◽  
Gilchrist A. L. Boly ◽  
Noufou Ouédraogo ◽  
Aristide Traoré ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Reducing Power ◽  
Ethyl Acetate Fraction ◽  
Health Science ◽  
Maximum Effect ◽  
Anti Inflammatory ◽  
Saba Senegalensis ◽  
Analgesic Activities

Aims: To evaluate antioxidant, anti-inflammatory and analgesic activities of extracts of Saba senegalensis leaves. Study Design: In vitro antioxidant assay and in vivo anti-inflammatory, analgesic assay of Saba senegalensis extracts. Place and Duration of Study: Saba senegalensis leaves, were collected in the Centre Region of Burkina Faso, in June–July 2015. The experiments were conducted at the department of Medicine and Traditional Pharmacopeia-Pharmacy (MEPHATRA-PH) of Institute of Research in Health Science (IRSS). Methodology: The anti-oedematous tests with carrageenan and the analgesic with acetic acid and investigate effect on isolated organ were carried out. The standards were acetylsalicylic acid and paracetamol. Results: After five hours of carrageenan-induced edema test, aqueous decoction (AD) presented better inhibition on all measure. In fact, at the different doses of 200 mg/kg, 400 mg/kg, and                600 mg/kg it presented percentages of inhibitions respectively of 30.81%, 62.27% and 72.71%. For the analgesic test, the hydroethanol macerate (HEM) showed a better pain reduction compared to the AD with a maximum effect of 77.28% at 400 mg/kg. Antioxidant activity with AD and his fractions shows that AD showed a better activity for the DPPH assay with an IC50 of 1.74 ±                0.10 μg/mL and a reducing power of 59.53 ± 2.16 mmol ET/g Sample. For HEM and his fractions, the ethyl acetate fraction (FHEM-AcOEt) showed a better IC50 of 0.18 ± 0.01 μg/mL for the DPPH test and dichloromethane fraction (FHEM-DCM) a reducing power agent of 88.88 ± 2.65 mmol ET/g Sample. All fractions were endowed with antioxidant properties by both methods. Conclusion: The study findings suggest that the presence of phenolic and terpenoid compounds could explain the antioxidant, anti-inflammatory and analgesic properties of these extracts.

scholarly journals Bioactive Bioflavonoids from Platonia insignis (Bacuri) Residues as Added Value Compounds

2021 ◽  
Author(s):  
Dayane Ribeiro ◽  
Helena Russo ◽  
Karina Fraige ◽  
Maria Zeraik ◽  
Cláudio Nogueira ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Solid Phase ◽  
Biological Activities ◽  
Drug Carrier ◽  
Antioxidant Properties ◽  
Added Value ◽  
Main Compound ◽  
Anti Inflammatory

Platonia insignis fruit, popularly known as bacuri, is traditionally used in folk medicine for its anti-inflammatory and antioxidant properties. Therefore, this study determined the chemical composition and biological activities of the bacuri’s shell and seeds extracts, considered residues from its consumption and industrial uses. Four biflavonoids (GB-2a, GB-1a, morelloflavone, and volkensiflavone) were identified in the extracts by high-performance liquid chromatography-diode array detection (HPLC-DAD), liquid chromatography tandem mass spectrometry (LC-MS/MS), and liquid chromatography-solid phase extraction-nuclear magnetic resonance (LC-SPE-NMR) techniques. Morelloflavone was identified as the main compound in the shell ethyl acetate extract, being responsible for the high in vitro antioxidant (50% effective concentration (EC50) ranging from 8.0-10.5 µg mL−1 in different protocols), anti-glycant (80%), and moderate inhibition of nitric oxide (1.56 µg mL−1 for > 90% cell viability) activities. This extract showed promising in vivo anti-inflammatory activity evaluated through the paw edema protocol after its incorporation into a liquid-crystalline drug carrier system, reducing the edema by up to 31%. The results demonstrated the potential of the fruit for the development of drugs of natural origin and corroborated to add economic value to these discarded residues.

scholarly journals Quercetin Liposomal Nanoformulation for Ischemia and Reperfusion Injury Treatment

Pharmaceutics ◽  
2022 ◽  
Vol 14 (1) ◽  
pp. 104
Author(s):  
Margarida Ferreira-Silva ◽  
Catarina Faria-Silva ◽  
Manuela C. Carvalheiro ◽  
Sandra Simões ◽  
Helena Susana Marinho ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Enhanced Recovery ◽  
Antioxidant Properties ◽  
Therapeutic Index ◽  
Drug Carriers ◽  
Ischemia And Reperfusion ◽  
Ischemia And Reperfusion Injury ◽  
Anti Inflammatory

Ischemia and reperfusion injury (IRI) is a common complication caused by inflammation and oxidative stress resulting from liver surgery. Current therapeutic strategies do not present the desirable efficacy, and severe side effects can occur. To overcome these drawbacks, new therapeutic alternatives are necessary. Drug delivery nanosystems have been explored due to their capacity to improve the therapeutic index of conventional drugs. Within nanocarriers, liposomes are one of the most successful, with several formulations currently in the market. As improved therapeutic outcomes have been demonstrated by using liposomes as drug carriers, this nanosystem was used to deliver quercetin, a flavonoid with anti-inflammatory and antioxidant properties, in hepatic IRI treatment. In the present work, a stable quercetin liposomal formulation was developed and characterized. Additionally, an in vitro model of ischemia and reperfusion was developed with a hypoxia chamber, where the anti-inflammatory potential of liposomal quercetin was evaluated, revealing the downregulation of pro-inflammatory markers. The anti-inflammatory effect of quercetin liposomes was also assessed in vivo in a rat model of hepatic IRI, in which a decrease in inflammation markers and enhanced recovery were observed. These results demonstrate that quercetin liposomes may provide a significant tool for addressing the current bottlenecks in hepatic IRI treatment.

scholarly journals Recent Advances on the Anti-Inflammatory and Antioxidant Properties of Red Grape Polyphenols: In Vitro and In Vivo Studies

Antioxidants ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 35 ◽  
Author(s):  
Thea Magrone ◽  
Manrico Magrone ◽  
Matteo Antonio Russo ◽  
Emilio Jirillo
Keyword(s):  
T Regulatory Cells ◽  
Antioxidant Properties ◽  
In Vivo Studies ◽  
Low Grade ◽  
Regulatory Cells ◽  
Grape Polyphenols ◽  
Anti Inflammatory ◽  
Red Grape

In this review, special emphasis will be placed on red grape polyphenols for their antioxidant and anti-inflammatory activities. Therefore, their capacity to inhibit major pathways responsible for activation of oxidative systems and expression and release of proinflammatory cytokines and chemokines will be discussed. Furthermore, regulation of immune cells by polyphenols will be illustrated with special reference to the activation of T regulatory cells which support a tolerogenic pathway at intestinal level. Additionally, the effects of red grape polyphenols will be analyzed in obesity, as a low-grade systemic inflammation. Also, possible modifications of inflammatory bowel disease biomarkers and clinical course have been studied upon polyphenol administration, either in animal models or in clinical trials. Moreover, the ability of polyphenols to cross the blood–brain barrier has been exploited to investigate their neuroprotective properties. In cancer, polyphenols seem to exert several beneficial effects, even if conflicting data are reported about their influence on T regulatory cells. Finally, the effects of polyphenols have been evaluated in experimental models of allergy and autoimmune diseases. Conclusively, red grape polyphenols are endowed with a great antioxidant and anti-inflammatory potential but some issues, such as polyphenol bioavailability, activity of metabolites, and interaction with microbiota, deserve deeper studies.

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