scholarly journals In vivo antimalarial activity of crude aqueous leaf extract of Pyrenacantha staudtii against Plasmodium berghei (NK65) in infected mice

2014 ◽  
Vol 8 (12) ◽  
pp. 342-345 ◽  
Author(s):  
O. F Olorunniyi, ◽  
A. Morenikeji O.
Keyword(s):  
Plasmodium Berghei ◽  
Leaf Extract ◽  
Antimalarial Activity ◽  
Aqueous Leaf Extract

scholarly journals Antimalarial Activity of Meriandra dianthera Leaf Extracts in Plasmodium berghei-Infected Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-8 ◽  
Author(s):  
Kalay Hagazy ◽  
Gereziher G. Sibhat ◽  
Aman Karim ◽  
Gebretsadkan H. Tekulu ◽  
Gomathi Periasamy ◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Leaf Extract ◽  
Antimalarial Activity ◽  
Negative Control ◽  
Mice Model ◽  
Oral Toxicity ◽  
Leaf Extracts ◽  
Traditional Use ◽  
Crude Leaf Extract

Objective. To evaluate the antimalarial effect of aqueous methanolic extract and solvent fractions of Meriandra dianthera leaves against Plasmodium berghei in mice model. Method. M. dianthera leaves were extracted with 80% methanol and dried. The dried crude extract was then defatted and further fractionated with chloroform, ethyl acetate, and butanol. Acute oral toxicity test was performed as per the Organization for Economic Cooperation and Development guideline 425. Peter’s 4-day suppressive test was used to determine the in vivo antimalarial activity of the extract and fractions. Result. The crude leaf extract of Meriandra dianthera showed parasite inhibition of 42.28% and 45.52% at doses of 400 and 600 mg/kg, respectively, as compared to the negative control. Moreover, the mice which received chloroform and aqueous fractions at the dose of 400 mg/kg/day showed significant (P<0.001) chemosuppression compared to the negative control. Both the extract and fractions were able to prevent P. berghei induced body weight loss and body temperature reduction and also increased the survival time of the mice as compared to the negative control. The aqueous methanolic leaf extract of M. dianthera showed no gross signs of toxicity or mortality in mice until a single oral dose of 2000 mg/kg. Conclusion. The extracts of M. dianthera leaves showed promising antimalarial activity, with no sign of toxicity and therefore may support its traditional use for the treatment of malaria.

scholarly journals In VivoAntimalarial Activity ofAnnona muricataLeaf Extract in Mice Infected withPlasmodium berghei

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Voravuth Somsak ◽  
Natsuda Polwiang ◽  
Sukanya Chachiyo
Keyword(s):  
Survival Time ◽  
Leaf Extract ◽  
Antimalarial Activity ◽  
Positive Control ◽  
Annona Muricata ◽  
The World ◽  
Aqueous Leaf Extract ◽  
New Compounds ◽  
Dose Dependent

Malaria is one of the most important infectious diseases in the world. The choice for the treatment is highly limited due to drug resistance. Hence, finding the new compounds to treat malaria is urgently needed. The present study was attempted to evaluate the antimalarial activity of theAnnona muricataaqueous leaf extract inPlasmodium bergheiinfected mice. Aqueous leaf extract ofA. muricatawas prepared and tested for acute toxicity in mice. For efficacy testin vivo, standard 4-day suppressive test was carried out. ICR mice were inoculated with 107parasitized erythrocytes ofP. bergheiANKA by intraperitoneal injection. The extracts (100, 500, and 1000 mg/kg) were then given orally by gavage once a day for 4 consecutive days. Parasitemia, percentage of inhibition, and packed cell volume were subsequently calculated. Chloroquine (10 mg/kg) was given to infected mice as positive control while untreated control was given only distilled water. It was found thatA. muricataaqueous leaf extract at doses of 100, 500, and 1000 mg/kg resulted in dose dependent parasitemia inhibition of 38.03%, 75.25%, and 85.61%, respectively. Survival time was prolonged in infected mice treated with the extract. Moreover, no mortality to mice was observed with this extract up to a dose of 4000 mg/kg. In conclusion, theA. muricataaqueous leaf extract exerted significant antimalarial activity with no toxicity and prolonged survival time. Therefore, this extract might contain potential lead molecule for the development of a new drug for malaria treatment.

scholarly journals Antimalarial Effect of the Total Glycosides of the Medicinal Plant, Ranunculus japonicus

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 532
Author(s):  
Hae-Soo Yun ◽  
Sylvatrie-Danne Dinzouna-Boutamba ◽  
Sanghyun Lee ◽  
Zin Moon ◽  
Dongmi Kwak ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Plasmodium Berghei ◽  
Antimalarial Activity ◽  
Hematological Parameters ◽  
Significant Inhibition ◽  
Ic50 Values ◽  
The Republic

In traditional Chinese medicine, Ranunculus japonicus has been used to treat various diseases, including malaria, and the young stem of R. japonicus is consumed as a food in the Republic of Korea. However, experimental evidence of the antimalarial effect of R. japonicus has not been evaluated. Therefore, the antimalarial activity of the extract of the young stem of R. japonicus was evaluated in vitro using both chloroquine-sensitive (3D7) and chloroquine-resistant (Dd2) strains; in vivo activity was evaluated in Plasmodium berghei-infected mice via oral administration followed by a four-day suppressive test focused on biochemical and hematological parameters. Exposure to extracts of R. japonicus resulted in significant inhibition of both chloroquine-sensitive (3D7) and resistant (Dd2) strains of P. falciparum, with IC50 values of 6.29 ± 2.78 and 5.36 ± 4.93 μg/mL, respectively. Administration of R. japonicus also resulted in potent antimalarial activity against P. berghei in infected mice with no associated toxicity; treatment also resulted in improved hepatic, renal, and hematologic parameters. These results demonstrate the antimalarial effects of R. japonicus both in vitro and in vivo with no apparent toxicity.

scholarly journals Sequestration of erythrocytes infected with Plasmodium berghei ANKA in BALB/c mice treated with goat bile

2020 ◽  
Vol 4 (2) ◽  
pp. 179
Author(s):  
Kartika Arum Wardani ◽  
Kholida Nur Aini ◽  
Heny Arwati ◽  
Willy Sandhika
Keyword(s):  
Plasmodium Berghei ◽  
Antimalarial Activity ◽  
Negative Control ◽  
Control Group ◽  
Dependent Manner ◽  
Plasmodium Berghei Anka ◽  
Concentration Dependent Manner ◽  
Control Group Design ◽  
Bile Concentration

Abstract Sequestration of Plasmodium berghei ANKA-infected erythrocytes occurs in BALB/c mice as characteristic of  Plasmodium falciparum infection in humans. Animals’ bile has been widely used for centuries in Traditional Chinese Medicine. Goat bile has been used in healing infectious and non-infectious diseases; however, no report on the use of goat bile against malaria infection and sequestration. The purpose of this study was to analyze the correlation between parasitemia and sequestration in the liver of P.berghei ANKA-infected BALB/c mice treated with goat bile. This research was an in vivo experimental study using the post-test control group design. The male BALB/c mice aged ± 6 weeks, body weight 20-25 g were used. The mice were divided into five groups where Group 1-3 were mice treated with goat bile 25%, 50%, and 100%, respectively. Group 4-5 were negative (sterile water) and positive controls (DHP). Parasitemia was observed daily from each mouse and the number of sequestered infected erythrocytes on the endothelium of sinusoids. The data were analyzed using t independent test. Antimalarial activity of goat bile was shown by the lower parasitemia in goat bile-treated mice compared with the negative control. The average number of sequestration was goat bile concentration-dependent manner. The higher the concentration, the lower the number of sequestration. Sequestration was correlated with parasitemia (p=0,0001). Sequestration of P.berghei ANKA-infected erythrocytes correlated with parasitemia, and was goat bile concentration-dependent manner. Keywords: Malaria, parasitemia, sequestration, goat bileCorrespondence: [email protected]

scholarly journals A Pilot Study on Antimalarial Effects of Moringa oleifera Leaf Extract in Plasmodium berghei Infection in Mice

2017 ◽  
Vol 15 (2) ◽  
pp. 151-156
Author(s):  
Somrudee NAKINCHAT ◽  
Voravuth SOMSAK
Keyword(s):  
Plasma Glucose ◽  
Crude Extract ◽  
Plasmodium Berghei ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Dependent Manner ◽  
Glucose Levels ◽  
New Compounds ◽  
Dose Dependent

The emergence and spread of antimalarial drug resistance of Plasmodium parasites, as well as hypoglycemia, during malaria infection, and subsequent death, are critical problems in malaria-endemic areas. Hence, finding new compounds, especially plant extracts having antimalarial and anti-hypoglycemic activities, are urgently needed. The present study aimed to investigate the antimalarial and anti-hypoglycemic effects of Moringa oleifera leaf extract in Plasmodium berghei infection in mice. Aqueous crude extract of M. oleifera leaves was freshly prepared and used for an efficacy test in vivo. Groups of ICR mice (5 mice in each) were infected with 1´107 infected red blood cells of P. berghei ANKA by intraperitoneal injection and given the extract orally with doses of 100, 500, and 1000 mg/kg for 4 consecutive days. Parasitemia and plasma glucose levels were subsequently measured. The results showed that M. oleifera leaf extract presented significant (p < 0.001) inhibition of parasitemia in a dose-dependent manner. Moreover, this extract exerted anti-hypoglycemia effects in infected mice in a dose-dependent manner. The highest degrees of activity were found at a dose of 1000 mg/kg of the extract. Additionally, no effect on plasma glucose was found in normal mice treated with this extract. It can be concluded that aqueous crude extract of M. oleifera leaves exerted antimalarial and anti-hypoglycemic effects in P. berghei infection in mice.

scholarly journals In Vivo Antiplasmodial Activity of Hydromethanolic Leaf Extract and Solvent Fractions of Maytenus gracilipes (Celastraceae) against Plasmodium berghei in Mice

Heliyon ◽  
2021 ◽  
pp. e08457
Author(s):  
Dejen Nureye ◽  
Muktar Sano Kedir ◽  
Rekik Ashebir Muluye ◽  
Workineh Woldeselassie Hammeso ◽  
Eyob Tekalign
Keyword(s):  
Plasmodium Berghei ◽  
Leaf Extract ◽  
Antiplasmodial Activity

scholarly journals Antimalaria and Hematological Properties of Ethanol Leaf Extract of Pennisetum purpureum on Plasmodium berghei Infected Mice

2019 ◽  
pp. 1-8
Author(s):  
E. N. Ekene ◽  
O. M. Odigie
Keyword(s):  
Body Weight ◽  
Plasmodium Berghei ◽  
Leaf Extract ◽  
Antimalarial Activity ◽  
Pennisetum Purpureum ◽  
High Dose ◽  
Control Groups ◽  
Parasite Count ◽  
Genus Plasmodium ◽  
Group I

Through bite from a female Anopheles mosquito, Malaria is transmitted by infection with single-celled parasites of the genus Plasmodium. Studies have shown it to be characterized by periodic bouts of severe chills, accompanied with high fever. It has been suggested that Pennisetum purpureum possess antiplasmodial effects, however, no scientific record(s) yet exist(s) to validate this claim. This study was therefore undertaken to determine the anti-malaria and haematological properties of ethanol leaf extract of P. purpureum in Plasmodium berghei -infected mice. Thirty-Five (35) albino mice (20g) were procured, acclimatized (for two weeks) and assigned to five groups of 7 mice each. With group I receiving standard rat feed ad-libitum (control), Groups II through V were respectively infected with Plasmodium berghei (malaria infected, untreated), Plasmodium berghei infected + treated with 5mg/kg body weight of Artesunate (malaria infected, Artesunate treated), infected with Plasmodium berghei + treated with 200mg/kg body weight of Pennisetum  purpureum (malaria infected, low dose extract treated), and infected with Plasmodium berghei + treated with 400mg/kg body weight of Pennisetum  purpureum (malaria infected, high dose extract treated). After 21 days of administration, mice were sacrificed, blood samples collected, centrifuged for 10 minutes at 300g, and resulting supernatant biochemically analysed for hematologic changes. Result showed a significant increase in initial parasite count across groups except control. Administration of Artesunate also caused a significant (p < .05) reduction in parasite counts upon comparison with control. More so, administration of low and high dose extract caused a significant (p < .05) reduction in parasite count following comparison with control. Administration of 200mg/kg caused the highest parasitemia suppression than high dose. We recommend for further evaluation of the plant in other to identify active ingredients responsible for the observed antimalarial activity.

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