scholarly journals Determination of mecA expression and other resistance mechanisms in methicillin-resistant Staphylococcus aureus isolated from Oreochromis niloticus (Nile tilapia)

2016 ◽  
Vol 10 (14) ◽  
pp. 481-485
Author(s):  
K Soliman Magdy ◽  
Abozahra Rania ◽  
Y Gaafar Alkhateib ◽  
M Younes Abdelgayed ◽  
A Affr Nawal
Keyword(s):  
Staphylococcus Aureus ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Resistance Mechanisms ◽  
Methicillin Resistant

scholarly journals Phenotypic and Molecular Typing of Nosocomial Methicillin-Resistant Staphylococcus aureus Strains Susceptible to Gentamicin Isolated in France from 1995 to 1997

2000 ◽  
Vol 38 (1) ◽  
pp. 185-190
Author(s):  
Jacques-Olivier Galdbart ◽  
Anne Morvan ◽  
Nevine El Solh
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Typing ◽  
Insertion Sequence ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Phage Typing ◽  
Methicillin Resistant ◽  
Before And After ◽  
Resistant Strains ◽  
Mrsa Strains

ABSTRACT Methicillin-resistant strains susceptible to gentamicin (Gm s MRSA) have emerged since 1993 in several French hospitals. To study whether particular clones have spread in various French cities and whether some clones are related to gentamicin-resistant (Gm r ) MRSA strains, various methods (antibiotyping, phage typing, determination of Sma I macrorestriction patterns before and after hybridization with IS 256 transposase and aacA-aphD probes) were used to compare 62 Gm s MRSA strains isolated from 1995 to 1997 in nine cities and 15 Gm r MRSA strains. Eighteen major Sma I genotypes were identified, of which 11 included only Gm s MRSA strains and 5 included only Gm r MRSA strains. Each of the Gm r MRSA strains contained 6 to 13 Sma I fragments hybridizing with the insertion sequence IS 256 , of which a single band also hybridized with the aacA-aphD gene. No such hybridizing sequences were detected in 60 of the 62 Gm s MRSA strains. Thus, the divergence between Gm r and Gm s MRSA strains is revealed, not only by their distributions in distinct Sma I genotypes but also by the differences in hybridization patterns. Two of the 62 Gm s MRSA strains had the uncommon feature of carrying several Sma I bands hybridizing with IS 256 , suggesting that they are possibly related to the Gm r MRSA strains grouped in the same Sma I genotype. Five of the 11 Sma I genotypes including only Gm s MRSA strains contained strains from diverse cities, isolated during different years and with different antibiograms, suggesting that some clones have spread beyond their cities of origin and persisted.

scholarly journals Allyl isothiocyanate from crucifers potentiates β-lactam activity against methicillin-resistant Staphylococcus aureus

2014 ◽  
Vol 2 (4) ◽  
pp. 189-193
Author(s):  
Sultan Beevi S ◽  
Vikram B ◽  
Lakshmi Narasu M
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Allyl Isothiocyanate ◽  
Resistance Mechanisms ◽  
Clinical Isolates ◽  
Health Concern ◽  
Multi Drug Resistance ◽  
Methicillin Resistant ◽  
Potentiation Effect ◽  
Broth Microdilution Method

Introduction: Increasing prevalence of methicillin-resistant Staphylococcus aureus (MRSA) worldwide with limited therapeutic options is a growing public health concern. Isothiocyanates (ITCs) from crucifers have been shown to possess antibacterial actions against MRSA by antagonizing its resistance mechanisms. Allyl isothiocyanates (AITC) is the predominant isothiocyanates (ITC) of commonly consumed cruciferous vegetables such as brussels, mustard, cabbage, cauliflower and kale. Objectives: The aim of this study is to evaluate the potentiation effect of AITC on ampicillin and cefixime against clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) using broth microdilution method and checkerboard methods. Results: AITC showed a promising antibacterial activity against a panel of clinical isolates of MRSA when used in alone. However, β-lactam antibiotics like ampicillin and cefixime had little or no activity against MRSA confirming their multi-drug resistance. When AITC combined with ampicillin and cefixime, MIC was reduced by ≥ 4 fold as compared to their monotherapy, evidencing a synergistic effect of AITC, as defined by a FICI of ≤0.5. Conclusions: AITC showed promising synergistic and potentiation effect on ampicillin and cefixime at sub-MIC level against multi-drug resistant MRSA. This modulatory effect of AITC on β-lactam antibiotics could be useful as a synergistic therapeutic pair in combating MRSA infection in a hospital or community settings.

scholarly journals Determination of Vancomycin and Daptomycin MICs by Different Testing Methods for Methicillin-Resistant Staphylococcus aureus

2011 ◽  
Vol 49 (6) ◽  
pp. 2272-2273 ◽  
Author(s):  
Mark C. Kruzel ◽  
Cole T. Lewis ◽  
Kerry J. Welsh ◽  
Evan M. Lewis ◽  
Nicola E. Dundas ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Testing Methods ◽  
Methicillin Resistant
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