scholarly journals Characteristics of 32 Acinetobacter baumanni isolates co-harboring OXA-Carbapenemases and 16S rRNA methylase gene

2017 ◽  
Vol 11 (4) ◽  
pp. 110-116
Author(s):  
John Roberts Padde ◽  
Suleiman Hassan Jama ◽  
Li Xiaohan ◽  
Li Mingcheng
Keyword(s):  
16S Rrna ◽  
Methylase Gene ◽  
16S Rrna Methylase

Emergence of 16S rRNA methylase gene armA and cocarriage of blaIMP-1 in Pseudomonas aeruginosa isolates from South Korea

2010 ◽  
Vol 68 (4) ◽  
pp. 468-470 ◽  
Author(s):  
Mamata Gurung ◽  
Dong Chan Moon ◽  
Migma Dorji Tamang ◽  
Jungmin Kim ◽  
Yoo Chul Lee ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
16S Rrna ◽  
South Korea ◽  
Methylase Gene ◽  
16S Rrna Methylase

scholarly journals Draft Genome Sequence of a Sequence Type 11 Klebsiella pneumoniae Clinical Strain Carrying a blaKPC-2 Carbapenemase Gene and an rmtB 16S rRNA Methylase Gene

2017 ◽  
Vol 5 (6) ◽  
Author(s):  
Zhihong Yao ◽  
Yu Feng ◽  
Li Wei ◽  
Zhiyong Zong
Keyword(s):  
16S Rrna ◽  
Klebsiella Pneumoniae ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Clinical Strain ◽  
Trna Genes ◽  
Carbapenemase Gene ◽  
Methylase Gene ◽  
16S Rrna Methylase

ABSTRACT Klebsiella pneumoniae strain WCHKP649, recovered from a human wound, carried the carbapenemase gene bla KPC-2 and 16S rRNA methylase gene rmtB. Here, we report its 5.6-Mb draft genome sequence, comprising 171 contigs with an average 57.34% G+C content. The genome contained 5,284 coding sequences and 84 tRNA genes.

Acquisition of 16S rRNA methylase gene in Pseudomonas aeruginosa

The Lancet ◽  
2003 ◽  
Vol 362 (9399) ◽  
pp. 1888-1893 ◽  
Author(s):  
Keiko Yokoyama ◽  
Yohei Doi ◽  
Kunikazu Yamane ◽  
Hiroshi Kurokawa ◽  
Naohiro Shibata ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
16S Rrna ◽  
Methylase Gene ◽  
16S Rrna Methylase

scholarly journals Emergence of 16S rRNA Methylase Gene rmtB in Salmonella Enterica Serovar London and Evolution of RmtB-Producing Plasmid Mediated by IS26

2021 ◽  
Vol 11 ◽  
Author(s):  
Jing Wang ◽  
Zhen-Yu Wang ◽  
Yan Wang ◽  
Fan Sun ◽  
Wei Li ◽  
...  
Keyword(s):  
16S Rrna ◽  
Single Molecule ◽  
Resistance Genes ◽  
Variable Region ◽  
Mobile Elements ◽  
Plasmid Evolution ◽  
Complete Sequences ◽  
Methylase Gene ◽  
16S Rrna Methylase

This study aimed to characterize 16S rRNA methylase genes among Salmonella and to elucidate the structure and evolution of rmtB-carrying plasmids. One hundred fifty-eight Salmonella isolates from one pig slaughterhouse were detected as containing 16S rRNA methylase genes; two (1.27%) Salmonella London isolates from slaughtered pigs were identified to carry rmtB. They were resistant to gentamicin, amikacin, streptomycin, ampicillin, tetracycline, florfenicol, ciprofloxacin, and sulfamethoxazole/trimethoprim. The complete sequences of RmtB-producing isolates were obtained by PacBio single-molecule real-time sequencing. The isolate HA1-SP5 harbored plasmids pYUHAP5-1 and pYUHAP5-2. pYUHAP5-1 belonged to the IncFIBK plasmid and showed high similarity to multiple IncFIBK plasmids from Salmonella London in China. The rmtB-carrying plasmid pYUHAP5-2 contained a typical IncN-type backbone; the variable region comprising several resistance genes and an IncX1 plasmid segment was inserted in the resolvase gene resP and bounded by IS26. The sole plasmid in HA3-IN1 designated as pYUHAP1 was a cointegrate of plasmids from pYUHAP5-1-like and pYUHAP5-2-like, possibly mediated by IS26 via homologous recombination or conservative transposition. The structure differences between pYUHAP1 and its corresponding part of pYUHAP5-1 and pYUHAP5-2 may result from insertion, deletion, or recombination events mediated by mobile elements (IS26, ISCR1, and ISKpn43). This is the first report of rmtB in Salmonella London. IncN plasmids are efficient vectors for rmtB distribution and are capable of evolving by reorganization and cointegration. Our results further highlight the important role of mobile elements, particularly IS26, in the dissemination of resistance genes and plasmid evolution.

scholarly journals Emergence of Various NDM-Type-Metallo-β-Lactamase-Producing Escherichia coli Clinical Isolates in Nepal

2017 ◽  
Vol 61 (12) ◽  
Author(s):  
Basudha Shrestha ◽  
Tatsuya Tada ◽  
Kayo Shimada ◽  
Shovita Shrestha ◽  
Hiroshi Ohara ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
16S Rrna ◽  
Clinical Isolates ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Methylase Gene ◽  
16S Rrna Methylase

ABSTRACT Of 250 clinical isolates of Escherichia coli obtained in Nepal, 38 were carbapenem resistant, with MICs of imipenem or meropenem of ≥4 μg/ml. All 38 isolates harbored the following bla NDMs: bla NDM-1, bla NDM-3, bla NDM-4, bla NDM-5, bla NDM-7, bla NDM-12, and bla NDM-13. Most of these isolates also harbored the 16S rRNA methylase gene(s) armA, rmtB, and/or rmtC.

scholarly journals Genetic Environment of 16S rRNA Methylase Gene rmtD

2008 ◽  
Vol 52 (6) ◽  
pp. 2270-2272 ◽  
Author(s):  
Yohei Doi ◽  
Jennifer M. Adams-Haduch ◽  
David L. Paterson
Keyword(s):  
Pseudomonas Aeruginosa ◽  
16S Rrna ◽  
Klebsiella Pneumoniae ◽  
Class I ◽  
Genetic Environment ◽  
Methylase Gene ◽  
16S Rrna Methylase

ABSTRACT The genetic environment of the 16S rRNA methylase gene rmtD was investigated. rmtD was flanked by a novel ISCR motif located downstream of class I integron In163 in the original Pseudomonas aeruginosa strain. rmtD found in Klebsiella pneumoniae appeared to have been mobilized from P. aeruginosa by an IS26-mediated event.

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