scholarly journals Isolation and characterization of antibiotic producing Bacillus species in Lake Bogoria, Kenya

2015 ◽  
Vol 9 (14) ◽  
pp. 1037-1043 ◽  
Author(s):  
Kintet Torome Tom ◽  
Gomezgani Matasyoh Lexa ◽  
Orinda George ◽  
Gakuya Francis
Keyword(s):  
Bacillus Species ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of Bacillus endophytic strains producers for non ribosomal lipopeptides NRLPs from tomato

2018 ◽  
Vol 9 (1) ◽  
pp. 128
Author(s):  
Walaa Hussein ◽  
Ramadan WA ◽  
Sameh Fahim
Keyword(s):  
High Performance ◽  
Plant Defence ◽  
Plant Diseases ◽  
Bacillus Species ◽  
Vegetable Crops ◽  
Defence Mechanisms ◽  
Isolation And Characterization ◽  
Genes Encoding ◽  
Plant Defence Mechanisms

Tomato (Solanum lycopersicum) are consid­ered one of the most important vegetable crops and infected by numbers of different diseases. Studying the use of biological alternatives, instead of chemical substances against plant diseases became necessary for the treatment by beneficial microorganisms endophytes, which can excrete natural products benefits to plant in reducing disease severity, promoting growth and inducing plant defence mechanisms. In this work, three endophytes strains were isolated from tomato stems and their 16srDNA have been found to belong to Bacillus species. The first strain was named BMG100, the second BMG101 and the third BMG102. Two Bacillus strains BMG100 and BMG101 have been found to harbour synthetases genes from three lipopeptides families; surfactin, plipastatin, and iturin (mycosubtilin) which can be detected by degenerated primers designed to detect the presence of synthetases genes encoding lipopeptides. The lipopeptides production was proved by their quantification using High Performance Liquid Chromatography (HPLC), whereas BMG100 produced 105, 178 and 293 mg/L of plipastatin, mycosubtilin and surfactin, respectively, BMG101 produced 385 mg/L of surfactin and 236 mg/L of mycosubtilin, while BMG102 showed no lipopeptides production. Keywords: Tomato; Endophytic bacteria; Lipopeptides; Bacillus species

scholarly journals Isolation and Characterization of Bacillus Species Possessing Antifungal Activity against Ginseng Root Rot Pathogens

2012 ◽  
Vol 16 (4) ◽  
pp. 357-363 ◽  
Author(s):  
Byung-Yong Kim ◽  
Jae-Hyung Ahn ◽  
Hang-Yeon Weon ◽  
Jaekyeong Song ◽  
Sung-Il Kim ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Root Rot ◽  
Ginseng Root ◽  
Bacillus Species ◽  
Isolation And Characterization

scholarly journals ISOLATION AND CHARACTERIZATION OF ACTIVE PROBIOTIC ORGANISMS IN FERMENTED ZEA MAYS, PENTADETHRA MACROPHYLLA BENTH,AND RICINUS COMMUNIS

2021 ◽  
Vol 9 (4) ◽  
pp. 512-516
Author(s):  
Sunday S. Agbem ◽  
Keyword(s):  
Ricinus Communis ◽  
Food Samples ◽  
Primary Objective ◽  
Fermented Food ◽  
Bacillus Species ◽  
Fermented Foods ◽  
Isolation And Characterization ◽  
Micro Organisms ◽  
Protein Rich

Active probiotic organisms are good bacteria considered to be live micro-organisms that are obtained from fermented foods. Evidence has shown that probiotics are essential in human health. This studys primary objective is to isolate and characterize the active probiotic organisms present in certain fermented food samples. Maize, African oil bean, and castor oil were subjected to analysis. The result found the presence of active probiotic organisms such as Pedioccocus, Lactobacillus, Micrococcus, and Bacillus species. The study concluded that these organisms are responsible for the fermentation of carbohydrates and protein-rich seeds.

Isolation and Characterization of Protease from Bacillus spp and Serratia spp

2013 ◽  
Vol 2 (6) ◽  
pp. 252-255
Author(s):  
Sri Latha Atmakuri ◽  
Priya R Iyer
Keyword(s):  
Bacillus Species ◽  
Feather Degradation ◽  
Leather Industry ◽  
Isolation And Characterization ◽  
Protease Enzyme ◽  
Gelatin Layer ◽  
Stain Removal ◽  
Bacillus Spp ◽  
Bioremediation Processes

Proteases are the largest group of enzymes that have a wide variety of industrialapplications in detergent, leather industry, pharmaceutical industry and bioremediation processes. In the current study, processed water from Pallavaramleather industry (Chennai & Tamilnadu) was used for isolation of organisms producing protease. Bacillus spp and Serratia spp were isolated on 0.5% casein minimal media. The presence of protease was confirmed by paper chromatography and estimated by ninhydrin method. The protease activity of Bacillus species was high at the temperature of 60oC, pH 9 and the concentration was found to be 280mg/100ml, whereas the activity of protease for Serratia species was high at the temperature of 40oC, pH 5 and at concentration of 240mg/100ml. The highest concentration at which the activity of protease was maximum was 2 % for both the organisms. The protease enzyme used for the blood stain removal and degradation of outer gelatin layer of X‐ray films was good however chicken feather degradation was less.

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