scholarly journals Evaluation of rice landraces against rice root-knot nematode, Meloidogyne graminicola

2015 ◽  
Vol 9 (16) ◽  
pp. 1128-1131 ◽  
Author(s):  
Ravindra H ◽  
Sehgal Mukesh ◽  
B Narasimhamurthy H ◽  
S Imran Khan H ◽  
A Shruthi S
Keyword(s):  
Rice Root ◽  
Root Knot Nematode ◽  
Meloidogyne Graminicola ◽  
Rice Landraces

scholarly journals Evaluation of Organic Amendments against Rice Root-KnotNematode at Seedling Stage of Rice

1970 ◽  
Vol 9 ◽  
pp. 21-27 ◽  
Author(s):  
Nabin Kumar Dangal ◽  
D. Sharma Poudyal ◽  
S. M. Shrestha ◽  
C. Adhikari ◽  
J. M. Duxbury ◽  
...  
Keyword(s):  
Block Design ◽  
Organic Amendments ◽  
Severity Index ◽  
Rice Root ◽  
Chicken Manure ◽  
Root Weight ◽  
Root Knot Nematode ◽  
Meloidogyne Graminicola ◽  
Shoot Weight ◽  
Lesion Severity

Pot experiment was conducted during July-September 2006 to evaluate some organic amendments such as sesame (Sesamum indicum) biomass, buckwheat (Fagopyrum esculentum) biomass, neem (Azadirachta indica) leaves, chinaberry (Melia azedarch) leaves and chicken manure @ 1, 2 and 3 t ha-1 each against the rice root-knot nematode (Meloidogyne graminicola Golden & Birchfield) in direct seeded rice. The treatments were replicated five times in a randomized complete block design. The number of second stage juveniles (J2) of M. graminicola was significantly low in chicken manure @ 3 t ha-1. The root knot severity index was significantly low in sesame @ 3 t ha-1, chinaberry @ 3, 2 or 1 t ha-1, neem @ 3 t ha-1 and chicken manure @ 2 or 3 t ha-1 amended soil but root lesion severity index was lower only in chicken manure @ 2 t ha-1 treated plots. The fresh shoot weight and length were significantly high in chicken manure amendment @ 2 or 3 t ha-1 at 45th day after seeding. However, the fresh root weight, length, number of leaves and number of J2 recovered from the roots were non-significant. Key words: biomass; juveniles; Meloidogyne graminicola; root-knot severity index; root lesion severity index DOI: 10.3126/njst.v9i0.3160 Nepal Journal of Science and Technology 9 (2008) 21-27

scholarly journals Laporan Pertama di Sulawesi Selatan: Karakter Morfologi dan Molekuler Nematoda Puru Akar yang Berasosiasi dengan Akar Padi di Kabupaten Wajo, Sulawesi Selatan

2018 ◽  
Vol 22 (1) ◽  
pp. 58
Author(s):  
Hishar Mirsam ◽  
Fitrianingrum Kurniawati
Keyword(s):  
Polymerase Chain Reaction ◽  
Nematode Species ◽  
Morphological Characters ◽  
Rice Root ◽  
Nucleotide Sequence Analysis ◽  
Morphological Identification ◽  
Root Knot Nematode ◽  
Chain Reaction ◽  
Meloidogyne Graminicola ◽  
Polymerase Chain

Root Knot Nematode (RKN) is one of the most important cosmopolite parasitic nematode species. Reports on RKN associated with rice root in Indonesia are still limited in West Java and Yogyakarta. This study aimed to identify the RKN associated with rice root in Sub-district of Bola, District of Wajo, South Sulawesi, based on morphological and molecular characters. Sampling was carried out by purposive method based on specific criteria of sample, i.e. root knot. Identification of root knot nematode (RKN) infestation in field was done by observing the primary and secondary symptoms. Morphological identification was carried out based on characters of juvenile 2 and the female perineal pattern. Molecular identification was based on amplification of r-DNA by polymerase chain reaction technique using primers rDNA2 and rDNA 1.58s. RKN were detected associated with the incidence of root knot in rice plant. RKN was identified as Meloidogyne graminicola based on morphological characters of juvenile 2 and the female perineal pattern. PCR using primer rDNA 2 / rDNA 1.58s successfully amplified a DNA band of RKN of ± 500 bp. Nucleotide sequence analysis showed that RKN isolated from Wajo was closely related to M. graminicola isolated from Nepal, China, India, Madagascar, and USA with homology of 98.1–100.00%. IntisariNematoda puru akar (NPA) merupakan salah satu jenis nematoda parasit penting yang bersifat kosmopolit. Laporan NPA yang berasosiasi dengan akar tanaman padi di Indonesia masih terbatas di Jawa Barat dan Yogyakarta. Penelitian ini bertujuan mengidentifikasi NPA yang berasosiasi dengan akar tanaman padi di Kabupaten Wajo, Sulawesi Selatan berdasarkan karakter morfologi dan molekuler. Pengambilan sampel dilakukan secara purposif dengan memilih sampel berdasarkan pada kriteria gejala spesifik penyakit puru akar. Identifikasi serangan dilakukan dengan mengamati gejala primer dan gejala sekunder. Identifikasi morfologi dilakukan dengan pengamatan karakter morfologi juvenil 2 dan pola perineal NPA betina.Identifikasi molekuler dilakukan dengan teknik polymerase chain reaction (PCR) untuk mengamplifikasi wilayah internal transcribed spacer (ITS) ribosomal DNA (rDNA) menggunakan pasangan primer rDNA2 dan rDNA1.58s. NPA ditemukan berasosiasi dengan akar tanaman padi yang memperlihatkan gejala puru akar. NPA diidentifikasi sebagai Meloidogyne graminicola berdasarkan karakter morfologi juvenil 2 dan pola perineal NPA betina. PCR menggunakan primer rDNA2/ rDNA1.58s berhasil mengamplifikasi pita DNA NPA dengan ukuran sekitar 500 bp. Analisis runutan nukleotida menunjukkan isolat NPA asal Wajo-Indonesia memiliki tingkat kekerabatan yang sangat dekat dengan isolat M. graminicola asal Nepal, Cina, India, Madagaskar, dan Amerika Serikat dengan nilai homologi berkisar 98,1–100,0%.

scholarly journals Genome structure and content of the rice root‐knot nematode ( Meloidogyne graminicola )

2020 ◽  
Vol 10 (20) ◽  
pp. 11006-11021 ◽  
Author(s):  
Ngan Thi Phan ◽  
Julie Orjuela ◽  
Etienne G. J. Danchin ◽  
Christophe Klopp ◽  
Laetitia Perfus‐Barbeoch ◽  
...  
Keyword(s):  
Genome Structure ◽  
Rice Root ◽  
Root Knot Nematode ◽  
Meloidogyne Graminicola

Development of real-time PCR primers for the quantification of Meloidogyne graminicola, Hirschmanniella oryzae and Heterodera cajani, pests of the major crops in Myanmar

Nematology ◽  
2016 ◽  
Vol 18 (3) ◽  
pp. 257-263 ◽  
Author(s):  
Akane Katsuta ◽  
Koki Toyota ◽  
Yu Yu Min ◽  
The Thiri Maung
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Central Area ◽  
Pcr Primers ◽  
Pigeon Pea ◽  
Rice Root ◽  
Root Knot Nematode ◽  
Meloidogyne Graminicola ◽  
Reliable Quantification ◽  
Heterodera Cajani

The rice root-knot nematode,Meloidogyne graminicola, and the rice root nematode,Hirschmanniella oryzae, are soil-borne pests causing damage to rice, and the pigeon pea cyst nematode,Heterodera cajani, is a pest to beans and sesame. Real-time PCR primers were designed for quantification. Relationships between the threshold cycle (Ct:y) values and number (no.) of nematodes inoculated () were:M. graminicola: ;H. oryzae: ; andH. cajani: .Meloidogyne graminicolaandH. oryzaewere detected in 25 and 38 out of 50 soils, collected from different fields in the lowland and central area of Myanmar, and their densities ranged from 1.0 to 4779 and from 0.4 to 787 (20 g soil)−1, respectively, whileH. cajaniwas detected only in two fields (3 and 268 (20 g soil)−1). The DNA-based method enables rapid and reliable quantification of the nematodes in soil.

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