scholarly journals Phylotype analysis of Ralstonia solanacearum strains causing potato bacterial wilt in Karnataka in India

2014 ◽  
Vol 8 (12) ◽  
pp. 1277-1281 ◽  
Author(s):  
Sagar Vinay ◽  
Singh Gurjar Malkhan ◽  
Arjunan Jeevalatha ◽  
R. Bakade Rahul ◽  
K. Chakrabarti S. ◽  
...  
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum

scholarly journals Ralstonia solanacearum Needs Motility for Invasive Virulence on Tomato

2001 ◽  
Vol 183 (12) ◽  
pp. 3597-3605 ◽  
Author(s):  
Julie Tans-Kersten ◽  
Huayu Huang ◽  
Caitilyn Allen
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Soft Agar ◽  
Cell Protein ◽  
In Planta ◽  
Tomato Plants ◽  
Bacterial Wilt Disease ◽  
Virulence Assay ◽  
Flagellar Filament

ABSTRACT Ralstonia solanacearum, a widely distributed and economically important plant pathogen, invades the roots of diverse plant hosts from the soil and aggressively colonizes the xylem vessels, causing a lethal wilting known as bacterial wilt disease. By examining bacteria from the xylem vessels of infected plants, we found thatR. solanacearum is essentially nonmotile in planta, although it can be highly motile in culture. To determine the role of pathogen motility in this disease, we cloned, characterized, and mutated two genes in the R. solanacearum flagellar biosynthetic pathway. The genes for flagellin, the subunit of the flagellar filament (fliC), and for the flagellar motor switch protein (fliM) were isolated based on their resemblance to these proteins in other bacteria. As is typical for flagellins, the predicted FliC protein had well-conserved N- and C-terminal regions, separated by a divergent central domain. The predicted R. solanacearum FliM closely resembled motor switch proteins from other proteobacteria. Chromosomal mutants lackingfliC or fliM were created by replacing the genes with marked interrupted constructs. Since fliM is embedded in the fliLMNOPQR operon, the aphAcassette was used to make a nonpolar fliM mutation. Both mutants were completely nonmotile on soft agar plates, in minimal broth, and in tomato plants. The fliC mutant lacked flagella altogether; moreover, sheared-cell protein preparations from the fliC mutant lacked a 30-kDa band corresponding to flagellin. The fliM mutant was usually aflagellate, but about 10% of cells had abnormal truncated flagella. In a biologically representative soil-soak inoculation virulence assay, both nonmotile mutants were significantly reduced in the ability to cause disease on tomato plants. However, the fliC mutant had wild-type virulence when it was inoculated directly onto cut tomato petioles, an inoculation method that did not require bacteria to enter the intact host from the soil. These results suggest that swimming motility makes its most important contribution to bacterial wilt virulence in the early stages of host plant invasion and colonization.

Effectiveness of Egyptian propolis on control of tomato bacterial wilt caused by Ralstonia solanacearum

2017 ◽  
Vol 124 (5) ◽  
pp. 467-472 ◽  
Author(s):  
Kamal A. M. Abo-Elyousr ◽  
Mohamed E. A. Seleim ◽  
Rafeek M. El-Sharkawy ◽  
Hadel M. M. Khalil Bagy
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Tomato Bacterial Wilt

scholarly journals Evaluation of biological efficacy of Trichoderma asperellum against tomato bacterial wilt caused by Ralstonia solanacearum

2018 ◽  
Vol 28 (1) ◽  
Author(s):  
Narasimhamurthy Konappa ◽  
Soumya Krishnamurthy ◽  
Chandra Nayaka Siddaiah ◽  
Niranjana Siddapura Ramachandrappa ◽  
Srinivas Chowdappa
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Trichoderma Asperellum ◽  
Biological Efficacy ◽  
Tomato Bacterial Wilt

scholarly journals First Report of Bacterial Wilt of Amaranth (Amaranthus cruentus) Caused by Ralstonia solanacearum in Benin

Plant Disease ◽  
2019 ◽  
Vol 103 (3) ◽  
pp. 578-578 ◽  
Author(s):  
R. Sikirou ◽  
M.-E. E. A. Dossoumou ◽  
B. Zocli ◽  
V. Afari-Sefa ◽  
J. Honfoga ◽  
...  
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Amaranthus Cruentus ◽  
First Report

scholarly journals Proteomic Approaches of Trichoderma hamatum to Control Ralstonia solanacearum Causing Pepper Bacterial Wilt

2015 ◽  
Vol 17 (06) ◽  
pp. 1101-1109 ◽  
Author(s):  
Peng Cheng ◽  
Wei Song ◽  
Xiao Gong ◽  
Yisong Liu ◽  
Weiguo Xie ◽  
...  
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Trichoderma Hamatum

scholarly journals Metabolomic Evaluation of Ralstonia solanacearum Cold Shock Protein Peptide (csp22)-Induced Responses in Solanum lycopersicum

2022 ◽  
Vol 12 ◽  
Author(s):  
Dylan R. Zeiss ◽  
Paul A. Steenkamp ◽  
Lizelle A. Piater ◽  
Ian A. Dubery
Keyword(s):  
Solanum Lycopersicum ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Plant Pathogens ◽  
Cold Shock ◽  
Phenylpropanoid Pathway ◽  
Infection Process ◽  
Cold Shock Protein ◽  
Pre Treatment ◽  
Molecular Patterns

Ralstonia solanacearum, the causal agent of bacterial wilt, is one of the most destructive bacterial plant pathogens. This is linked to its evolutionary adaptation to evade host surveillance during the infection process since many of the pathogen’s associated molecular patterns escape recognition. However, a 22-amino acid sequence of R. solanacearum-derived cold shock protein (csp22) was discovered to elicit an immune response in the Solanaceae. Using untargeted metabolomics, the effects of csp22-elicitation on the metabolome of Solanum lycopersicum leaves were investigated. Additionally, the study set out to discover trends that may suggest that csp22 inoculation bestows enhanced resistance on tomato against bacterial wilt. Results revealed the redirection of metabolism toward the phenylpropanoid pathway and sub-branches thereof. Compared to the host response with live bacteria, csp22 induced a subset of the discriminant metabolites, but also metabolites not induced in response to R. solanacearum. Here, a spectrum of hydroxycinnamic acids (especially ferulic acid), their conjugates and derivatives predominated as signatory biomarkers. From a metabolomics perspective, the results support claims that csp22 pre-treatment of tomato plants elicits increased resistance to R. solanacearum infection and contribute to knowledge on plant immune systems operation at an integrative level. The functional significance of these specialized compounds may thus support a heightened state of defense that can be applied to ward off attacking pathogens or toward priming of defense against future infections.

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