scholarly journals A new bioassay using Chlorella vulgaris cell density for detecting mycotoxins

2013 ◽  
Vol 7 (50) ◽  
pp. 5709-5712
Author(s):  
B Aboul Nasr M ◽  
A Zohri Abdel Naser ◽  
Mahmoud Amer Enas
2018 ◽  
Vol 39 (5(SI)) ◽  
pp. 747-753 ◽  
Author(s):  
T. Katayama ◽  
◽  
N. Nagao ◽  
M. Goto ◽  
F. Md. Yusoff ◽  
...  

2021 ◽  
Author(s):  
Imroatun Nafi'ah ◽  
Sitoresmi Prabaningtyas ◽  
Agung Witjoro ◽  
Yanis Kurnia Basitoh ◽  
Achmad Rodiansyah ◽  
...  

Abstract The consortium of various types of bacteria from lakes in East Java has the potency to stimulate microalgae Chlorella vulgaris growth. Increased microalgae density from co-culture has an excellent potency for sources of biomass that can be developed for renewable energy. Several stages conducted of this research started from an exploration of IAA producing bacteria and amylolytic bacteria from several East Java Lakes; then, the highest bacterial isolates were identified with morphological and genotypical characteristics. The well-characterized bacterial isolates were used for the microbial consortium in co-culture with C. vulgaris. The treatment used in this study as follows: I) C. vulgaris without bacteria culture as a control, II) amylolytic bacteria + C. vulgaris, III) IAA-producing bacteria + C. vulgaris, IV) potential amylolytic bacteria and IAA-producing bacteria + C. vulgaris. The exploration result of potential bacteria from Ranu Pani, Ranu Regulo, Telaga Ngebel, and Ranu Grati lakes was found 53 amylolytic bacterial isolates, and 90 isolates IAA-producing bacteria. The highest amylolytic bacteria (isolate L) is related to Bacillus amyloliquefaciens, while the most elevated IAA-producing bacteria (isolate C) is related to Bacillus paramycoides. The highest cell density was produced in treatment III, reaching 2.7 x 106 cells/mL on day 50th. The treatments with supplement bacteria showed a significant effect for accelerating the growth of microalgae compared to control.


2012 ◽  
Vol 161 (3) ◽  
pp. 242-249 ◽  
Author(s):  
Weiqi Fu ◽  
Olafur Gudmundsson ◽  
Adam M. Feist ◽  
Gisli Herjolfsson ◽  
Sigurdur Brynjolfsson ◽  
...  

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11217
Author(s):  
Chin Sze Yee ◽  
Victor Tosin Okomoda ◽  
Fakriah Hashim ◽  
Khor Waiho ◽  
Siti Rozaimah Sheikh Abdullah ◽  
...  

This study investigated the effect of co-culturing microalgae with a floc-forming bacterium. Of the six microalgae isolated from a biofloc sample, only Thalassiosira weissflogii, Chlamydomonas sp. and Chlorella vulgaris were propagated successfully in Conway medium. Hence, these species were selected for the experiment comparing microalgae axenic culture and co-culture with the floc-forming bacterium, Bacillus infantis. Results obtained showed that the co-culture had higher microalgae biomass compared to the axenic culture. A similar trend was also observed concerning the lipid content of the microalgae-bacterium co-cultures. The cell number of B. infantis co-cultured with T. weissflogii increased during the exponential stage until the sixth day, but the other microalgae species experienced a significant early reduction in cell density of the bacteria at the exponential stage. This study represents the first attempt at co-culturing microalgae with B. infantis, a floc-forming bacterium, and observed increased biomass growth and lipid accumulation compared to the axenic culture.


2014 ◽  
Vol 61 ◽  
pp. 2012-2015 ◽  
Author(s):  
Qitao Gong ◽  
Yuanzheng Feng ◽  
Ligai Kang ◽  
Mengyuan Luo ◽  
Junhong Yang

2021 ◽  
Author(s):  
Tahereh Ebrahini Yazdanabdad ◽  
Ali Forghaniha ◽  
Mozhgan Emtyazjoo ◽  
Majid Ramezani

Abstract This study investigated the effects of Fe3O4 nanoparticles released from synthesized Thiourea catalyst to Chlorella vulgaris as an essential primary producer in aquatic systems. A range of Fe3O4 concentrations (0, 10, 100, 250, 500, 750, and 1000 mg L-1) was applied for the exposure test. Biological parameters of C. vulgaris, including cell density, cell viability, and pigment content were assessed. Bioconcentration factor and bioaccumulation were evaluated for contaminated microalgae. Non-carcinogenic risks were then assessed using target hazard quotient (THQ) for potential human consumptions. Findings showed that C. vulgaris cell numbers increased from 0 to 500 mg L-1 of Fe3O4. Chlorophyll a represented a time-dependent response, and greatest values were detected in 250 and 500 mg L-1 Fe3O4 at 4.2 and 4 mg/g, respectively. Chlorophyll b content showed a time-related manner in exposure to Fe3O4 with the highest values recorded at 250 mg L-1 after 96 h. Moreover, bioaccumulation displayed a dose-dependent response as bioaccumulated iron was in the largest amount at 15000 µg/g dw in 1000 mg L-1, whereas the lowest one was in the control group at 1700 µg/g dw. The bioconcentration factor showed a concentration-relevant decrease in all iron treatments and 10 mg L-1 of Fe3O4 represented the greatest BCF at 327.3611. Non-carcinogenic risks illustrated negligible hazard (THQ < 1) in a dose-response pattern and the largest EDI and THQ were calculated in 1000 mg L-1 at 7.4332E-07 (mg kg-1 day-1) and 1.06189E-09, respectively. In essence, iron is an essential trace element for biological aspects in aquatic systems, but in exceeding concentrations could impose toxicity effects in C. vulgaris populations.


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