scholarly journals Ultrasound assisted extraction for analysis of total phenolic content and antioxidant activity of medicinal plants

2013 ◽  
Vol 6 (40) ◽  
pp. 6847-6852 ◽  
Author(s):  
Wang Xin Hong
2020 ◽  
Vol 16 (2) ◽  
pp. 124-132 ◽  
Author(s):  
Mahsa Souri ◽  
Alireza Shakeri

Background: Dittrichia graveolens (L.) is a strongly aromatic plant distributed in Mediterranean regions. This research concerns the optimization of the Total Phenolic Content (TPC) and the Total Tannin Content (TTC) of Dittrichia graveolens (L.) extracts using Response Surface Methodology for Ultrasound Assisted Extraction (UAE) and Microwave Assisted Extraction (MAE). Central Composite Design (CCD) was used to evaluate the effect of the solvent concentration and the extraction time, in different methods on TTC and TPC. The antioxidant activities and antibacterial activities were evaluated. Methods: The plant extracts were prepared using maceration, microwave and ultrasound assisted extraction. TPC and TTC were measured using Folin-Ciocalteu method. The antioxidant activities were studied using DPPH reagent and disc diffusion method was used to study the antibacterial activities. Results: This study showed the optimum condition for UAE was 49.96% methanol concentration and 11.2 min sonication, while for MAE was 55.44% methanol concentration and 2.26 min microwave extraction. It also indicated that MAE was the most effective method in comparison to UAE and maceration. The antioxidant activities of MAE extract (IC50=7.7mg/mL) were more than UAE extract (IC50=21.5mg/mL) and maceration (IC50=32.3mg/mL). Conclusion: As a conclusion, it was indicated that MAE was the most effective method. The higher total phenolic content caused higher antioxidant activities as MAE extract had the highest antioxidant activities. The antibacterial test showed the great potential of this plant as an antibacterial compound resource against different bacteria.


Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 10
Author(s):  
Irene Gómez-Cruz ◽  
Inmaculada Romero ◽  
María del Mar Contreras ◽  
Carmen Padilla-Rascón ◽  
Florbela Carvalheiro ◽  
...  

The exhausted olive pomace (EOP) is the main residue generated year-round after the extraction of the residual oil contained in the olive pomace. Due to its chemical composition, EOP is an interesting bioresource for the production of bioenergy and high added-value products. Therefore, the purpose of this study was to evaluate the extraction of antioxidants from EOP using sequential ultrasound-assisted extraction (UAE) and maceration with constant stirring. Water and 70% acetone were used as solvents. The total phenolic content was determined by the Folin–Ciocalteu method, the antioxidant capacity was investigated and the phenolic extracts were also characterized.


Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 248 ◽  
Author(s):  
Ivana Savic Gajic ◽  
Ivan Savic ◽  
Ivana Boskov ◽  
Stanko Žerajić ◽  
Ivana Markovic ◽  
...  

The aim of this study was to optimize the ultrasound-assisted extraction of phenolic compounds from black locust (Robiniae pseudoacaciae) flowers using central composite design. The ethanol concentration (33–67%), extraction temperature (33–67 °C), and extraction time (17–33 min) were analyzed as the factors that impact the total phenolic content. The liquid-to-solid ratio of 10 cm3 g−1 was the same during extractions. The optimal conditions were found to be 59 °C, 60% (v/v) ethanol, and extraction time of 30 min. The total phenolic content (TPC = 3.12 gGAE 100 g−1 dry plant material) and antioxidant activity (IC50 = 120.5 µg cm−3) of the extract obtained by ultrasound-assisted extraction were compared with those obtained by maceration (TPC = 2.54 gGAE 100 g−1 dry plant material, IC50 = 150.6 µg cm−3) and Soxhlet extraction (TPC = 3.22 gGAE 100 g−1 dry plant material, IC50 = 204.2 µg cm−3). The ultrasound-assisted extraction gave higher total phenolic content and better antioxidant activity for shorter extraction time so that it represents the technique of choice for the extraction of phenolic compounds. The obtained extract, as the source of antioxidants, can be applied in the pharmaceutical and food industries.


Processes ◽  
2020 ◽  
Vol 8 (12) ◽  
pp. 1530
Author(s):  
Renata Iara Cavalaro ◽  
Luis Felipe de Freitas Fabricio ◽  
Thais Maria Ferreira de Souza Vieira

Baccharis dracunculifolia or rosemary-of-field is the principal botanical source used by Africanized bees Apis mellifera L. to produce green propolis in Southeastern Brazil. The phenolic compounds present in the plant and green propolis have been reported to be responsible for biological activities such as antioxidant capacity. This study aimed to optimize the ultrasound-assisted extraction of antioxidants compounds from rosemary-of-field using a central composite rotatable design (CCRD), and compare results to green propolis extract. An experimental design was performed to obtain responses of total phenolic content and antioxidant capacity. The results allowed observing that the optimum condition for both Baccharis dracunculifolia floral bud and raw green propolis antioxidant extraction was obtained with 99% ethanol solution. In this condition, Total Phenolic Content (TPC), Ferric Reducing Antioxidant Power (FRAP), and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) values were 612.14 mg GAE. g−1 sample, 534.39 µM ferrous sulfate g−1 sample, and 72.37 µmol TEAC. g−1 sample, respectively, for floral buds. These results have confirmed that optimization is a key step for effective and sustainable extraction processes to be feasible on an industrial scale. The proposed process can be easily adopted on a larger scale, as it uses very few inputs and presents straightforward steps, with the advantage of high efficiency in the extraction of phenolic compounds from the studied matrices compared to the results from the literature. The high concentration of antioxidants indicates that the products obtained can be considered as a sustainable bioactive source for food and cosmetic purposes.


2020 ◽  
Vol 31 (1) ◽  
pp. 14-17 ◽  
Author(s):  
Serap Ayaz Seyhan ◽  
Zümrüt Yilmaz ◽  
Deniz Çikla Yilmaz

AbstractThe objective of this work was to compare the extraction of phenolic compounds from Tilia argentea flowers and bracts by using conventional (solvent extraction) and novel (ultrasound assisted) extraction methods. Ethanol (70 %) extracts were analyzed for their antioxidant activities. Total phenolic content was determined using Folin-Ciocalteu method and the antioxidant potential was determined by DPPH radical scavenging and Ferric Reducing Antioxidant Power (FRAP) assays. To determine the effect of ultrasound treatment on the extraction, same extraction parameters were applied in both methods. The results showed that extracts obtained by ultrasound assisted extraction have higher total phenolic content and antioxidant activity.


2020 ◽  
Vol 16 (1-2) ◽  
Author(s):  
Cassiano Brown da Rocha ◽  
Caciano Pelayo Zapata Noreña

AbstractThe grape pomace is a by-product from the industrial processing of grape juice, which can be used as a source of bioactive compounds. The aim of this study was to separate the phenolic compounds from grape pomace using an acidic aqueous solution with 2 % citric acid as a solvent, using both ultrasound-assisted extraction, with powers of 250, 350 and 450 W and times of 5, 10 and 15 min, and microwave-assisted extraction using powers of 600, 800 and 1,000 W and times of 5, 7 and 10 min. The results showed that for both methods of extraction, the contents of total phenolic compounds and antioxidant activity by ABTS and DPPH increased with time, and microwave at 1,000 W for 10 min corresponded to the best extraction condition. However, the contents of phenolic compounds and antioxidant activity were lower than exhaustive extraction using acidified methanol solution.


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