Isolation and characterization of lytic phage against Lactococcus lactis RP359, kem-buk-nud starter culture

2012 ◽  
Vol 6 (37) ◽  
Author(s):  
Parichat Phumkhachorn
Keyword(s):  
Lactococcus Lactis ◽  
Starter Culture ◽  
Lytic Phage ◽  
Isolation And Characterization

The first sequenced Sphaerotilus natans bacteriophage– characterization and potential to control its filamentous bacterium host

2021 ◽  
Vol 97 (4) ◽  
Author(s):  
Rute Ferreira ◽  
Rui Amado ◽  
Jorge Padrão ◽  
Vânia Ferreira ◽  
Nicolina M Dias ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Bacterial Communities ◽  
Potential Application ◽  
Deleterious Effect ◽  
Wastewater Treatment Plants ◽  
Lytic Phage ◽  
Filamentous Bacterium ◽  
Sphaerotilus Natans ◽  
Isolation And Characterization

ABSTRACT Bacteriophages (phages) are ubiquitous entities present in every conceivable habitat as a result of their bacterial parasitism. Their prevalence and impact in the ecology of bacterial communities and their ability to control pathogens make their characterization essential, particularly of new phages, improving knowledge and potential application. The isolation and characterization of a new lytic phage against Sphaerotilus natans strain DSM 6575, named vB_SnaP-R1 (SnaR1), is here described. Besides being the first sequenced genome of a Sphaerotilus natans infecting phage, 99% of its 41507 bp genome lacks homology with any other sequenced phage, revealing its uniqueness and previous lack of knowledge. Moreover, SnaR1 is the first Podoviridae phage described infecting this bacterium. Sphaerotilus natans is an important filamentous bacterium due to its deleterious effect on wastewater treatment plants (WWTP) and thus, phages may play a role as novel biotechnological tools against filamentous overgrowth in WWTP. The lytic spectrum of SnaR1 was restricted to its host strain, infecting only one out of three S. natans strains and infection assays revealed its ability to reduce bacterial loads. Results suggest SnaR1 as the prototype of a new phage genus and demonstrates its potential as a non-chemical alternative to reduce S. natans DSM 6575 cells.

scholarly journals Isolation and characterization of Lactobacillus plantarum BLS29 as a potential probiotic starter culture for pork sausage production

2015 ◽  
Vol 9 (32) ◽  
pp. 1887-1895 ◽  
Author(s):  
Senter Luciana ◽  
Queiroz Pereira Jamile ◽  
Alano Vieira Manoela ◽  
Maria Zandonai Michielin Eliane ◽  
Cesar Tondo Eduardo
Keyword(s):  
Lactobacillus Plantarum ◽  
Starter Culture ◽  
Pork Sausage ◽  
Isolation And Characterization

scholarly journals Characterization of the Two-Component Abortive Phage Infection Mechanism AbiT from Lactococcus lactis

2002 ◽  
Vol 184 (22) ◽  
pp. 6325-6332 ◽  
Author(s):  
Julie D. Bouchard ◽  
Eric Dion ◽  
Frédéric Bissonnette ◽  
Sylvain Moineau
Keyword(s):  
Lactococcus Lactis ◽  
Burst Size ◽  
Lytic Cycle ◽  
Phage Infection ◽  
Site Directed Mutagenesis ◽  
Hydrophobic Region ◽  
Plasmid Copy ◽  
Isolation And Characterization ◽  
Fold Reduction

ABSTRACT During the production of fermented dairy products, virulent bacteriophages infecting Lactococcus lactis can delay or stop the milk acidification process. A solution to this biological problem consists of introducing natural phage barriers into the strains used by the dairy industry. One such hurdle is called abortive infection (Abi) and causes premature cell death with no or little phage progeny. Here, we describe the isolation and characterization of a novel Abi mechanism encoded by plasmid pED1 from L. lactis. The system is composed of two constitutively cotranscribed genes encoding putative proteins of 127 and 213 amino acids, named AbiTi and AbiTii, respectively. Site-directed mutagenesis indicated that a hydrophobic region at the C-terminal extremity of AbiTi is essential to the antiphage phenotype. The AbiT system is effective against phages of the 936 and P335 species (efficiency of plaquing between 10−5 and 10−7) and causes a 20-fold reduction in the efficiency to form centers of infection as well as a 10- to 12-fold reduction in the burst size. Its efficacy could be improved by raising the plasmid copy number, but changing the intrinsic ratio of AbiTi and AbiTii did not greatly affect the antiphage activity. The monitoring of the intracellular phage infection process by DNA replication, gene expression, and electron microscopy as well as the study of phage mutants by genome mapping indicated that AbiT is likely to act at a later stage of the phage lytic cycle.

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