Prevalence assessment of Staphylococcus aureus and Streptococcus agalactiae by multiplex polymerase chain reaction (M-PCR) in bovine sub-clinical mastitis and their effect on somatic cell count (SCC) in Iranian dairy cows

2012 ◽  
Vol 6 (12) ◽  
Author(s):  
Mohammad Goli
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Dairy Cows ◽  
Cell Count ◽  
Streptococcus Agalactiae ◽  
Multiplex Polymerase Chain Reaction ◽  
Somatic Cell Count ◽  
Clinical Mastitis ◽  
Chain Reaction ◽  
Polymerase Chain

Development of a Multiplex Polymerase Chain Reaction Assay for Detection and Differentiation of Staphylococcus aureus in Dairy Products†

2001 ◽  
Vol 64 (5) ◽  
pp. 664-668 ◽  
Author(s):  
SUDHIR TAMARAPU ◽  
JOHN L. McKILLIP ◽  
MARYANNE DRAKE
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Multiplex Pcr ◽  
Dairy Products ◽  
Multiplex Polymerase Chain Reaction ◽  
Skim Milk ◽  
Cheddar Cheese ◽  
Polymorphism Analysis ◽  
Chain Reaction ◽  
Polymerase Chain

A multiplex polymerase chain reaction (PCR) assay was developed for the detection and differentiation of enterotoxigenic Staphylococcus aureus in dairy products. A solvent extraction procedure was successfully modified for extraction of S. aureus DNA from 10 ml of artificially contaminated skim milk or 20 g cheddar cheese. Primers targeting the enterotoxin C gene (entC) and thermostable nuclease gene (nuc) were used in the multiplex PCR. PCR products were confirmed using restriction fragment length polymorphism analysis. DNA was consistently quantified and amplified by uniplex PCR from 10 CFU/ml of S. aureus in skim milk or 10 CFU/20 g cheddar cheese. The sensitivity of the multiplex PCR was 100 CFU/ml of skim milk or 100 CFU/20 g cheddar cheese. The developed methodology allows presumptive identification and differentiation of enterotoxigenic S. aureus in less than 6 h.

Sign in / Sign up

Export Citation Format

Share Document